Antiproliferation Assay of Essential Oil of Curcuma Rhizoma (Curcuma xanthorrhiza Roxb.) Against P388 Leukemia Cell

Abstract

Leukemia or blood cancer is a disease which marked by abnormal increasing of blood producer`s cells. Chemotherapies which used as anticancer have a many adverse effect and toxicity. The volatile oil of turmeric rhizome (Curcuma xanthorriza) contains sesquiterpene which has an pharmacological activity. The aimed of this research to assay the antiproliferation activity of volatile oil from curcuma rhizome to leukemia P388 cells using MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl tetrazolium bromide) method. The methods were contain of identification of volatile oil (produced from distillation water-steam) using organoleptic test and TLC, and activity test was using seven various concentrations, which were 0.1; 0.3; 1; 3; 10; 30; 100 µg/mL. The result showed that the sample can inhibit leukemia P388 cells with the value of IC50 was 15.5 µg/mL. The volatile oil of Curcuma rhizome has an antiproliferative activity to leukemia P388 cell.Keywords: Curcuma rhizome, MTT assay, leukemia cell P388, volatile oil

Short Communication: Analysis of rhizome color and phytochemical content of 10 accessions of Curcuma zanthorrhiza Roxb. in Jambi, Indonesia

“…Most C. zanthorrhiza rhizomes contain various sesquiterpene derivatives, including alpha and beta turmerone derived from the essential oil. Various studies have shown that the essential oil content of the rhizomes is in the range of 7.3-29.5% on a dry weight basis (Musfiroh et al 2020). In addition to terpenoids and curcuminoids, 150 other compounds that are also known to be abundant in C. zanthorrhiza rhizomes are the sesquiterpene phenol xanthorrhizol compounds (32%); beta-curcumin (17%), zingiberene (13%) and beta bisabolol (3.5%).…”

“…In addition to terpenoids and curcuminoids, 150 other compounds that are also known to be abundant in C. zanthorrhiza rhizomes are the sesquiterpene phenol xanthorrhizol compounds (32%); beta-curcumin (17%), zingiberene (13%) and beta bisabolol (3.5%). Xanthorrhizol is the dominant compound from the sesquiterpene group of C. zanthorrhiza that has pharmacological activity against cancer cell metastatic activity and for inhibition of tumor cells (Musfiroh et al 2020).…”

The Effects of Javanese Turmeric (Curcuma xanthorriza Roxb) on Fibroblasts, Granulation, Blood Vessel Density, and Contraction in Wound Healing of STZ-Induced Diabetic Rats

“…Javanese turmeric (Curcuma xanthorrhiza Roxb) contains major metabolites such as curcuminoid, terpenoid, and xanthorrhizol essential oil that affect human organs, such as the gallbladder, liver, pancreas, rheumatism, stomach disease, and skin inflammation (Musfiroh et al, 2013). Xanthorrhizol from Curcuma xanthorrhiza Roxb has been reported as having antimicrobrial activity for S.aureus resistance (Kesumayadi et al, 2021).…”