Antiproliferative activity of daidzein and genistein may be related to ERα/c-erbB-2 expression in human breast cancer cells

Choi, Eun Jeong; Kim, Gun-Hee

doi:10.3892/mmr.2013.1283

Cited by 76 publications

(52 citation statements)

References 26 publications

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“…1), which is expected to interact effectively with estrogen receptors therefore inhibiting estrogen synthesis 17,57,58 and leading to a growth inhibition of MCF-7 cancer cells. In turn, for the MDA-MB-231 cells lacking estrogen receptors, daidzein is suggested to affect cell proliferation and viability by interference with different metabolic pathways, namely through cell cycle arrest (changes in regulatory proteins) and induction of apoptosis (via activation of caspases).…”

Section: Mcf-7mentioning

confidence: 99%

Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells

et al. 2016

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Breast cancer is the second most common type of cancer worldwide and the most frequent among women, being the fifth cause of death from neoplastic disease. Since this is an oxidative-stress related neoplasia, it is largely preventable. A dietary isoflavone abundant in soybean -daidzein -was currently investigated as to its chemopreventive and/or chemotherapeutic properties towards the human MDA-MB-231 (metastatic, estrogen-unresponsive) and MCF-7 (estrogen-responsive) breast cancer cell lines. Biological assays for evaluation of antitumour and anti-invasive activities were combined with state-of-theart vibrational microspectroscopy techniques. At 50 and 100 µM concentrations and 48 h incubation time, daidzein was found to induce a marked decrease in cell viability (ca. 50%) for MDA-MB-231 and MCF-7 cells (respectively ca. 50% and 42%) and 40% inhibition of cell migration 48 h. MicroRaman analysis of fixed cells upon exposure to this isoflavone unveiled its metabolic impact on both cell lines. Multivariate data analysis (unsupervised PCA) led to a clear discrimination between control and DAID-exposed cells, with distinctive effects on their biochemical profile, particularly regarding DNA, lipids and protein components, in a cell-dependent way. This is the first reported study on the impact of dietary antioxidants on cancer cells by microRaman techniques. A IntroductionCancer is one of the major causes of morbidity and mortality worldwide, closely following cardiovascular disorders. Breast carcinoma, in particular, is an important public health problem since it is the second most lethal cancer among women worldwide: according to the World Health Organisation (WHO), it affects 28% of the women in Europe 1 , being the second most lethal cancer type in Portugal (16%). 2 Epidemiological evidence has demonstrated a clear difference in breast cancer incidence between eastern and western countries, asian women being the least affected, which can be closely related to dietary habits.Phytochemicals, secondary plant metabolites widespread in fruits, vegetables, cereals and beverages such as tea, coffee and wine, and very abundant in a mediterranean diet, are an abundant source of dietary antioxidants known to significantly decrease the harmful effect of oxidative species, thus being promising chemopreventive agents towards carcinogenesis. [3][4][5][6] Actually, several studies have established a direct relationship between the intake of polyphenols and the prevention of oxidative stress-induced diseases, namely neurodegenerative and cardiovascular disorders, and some types of cancer. 7,8 The isoflavone daidzein (DAID), in particular, is the second most abundant component of soybean (following genistein), a growingly consumed food product, which renders this isoflavone a very suitable target of study regarding its potential health beneficial effects. In addition, it exhibits structural and functional similarities to the endogenous hormone estrogen 9 (Fig. 1). Since some breast cancers are estrogen-dependent (ER + ), DAID may...

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Section: Mcf-7mentioning

confidence: 99%

Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells

et al. 2016

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“…The abovementioned study reported that women consuming isoflavonecontaining supplements had a reduced risk for breast cancer in Ontario, Canada [11]. Clinical studies are also supported by in vitro studies indicating that isoflavones, mainly genistein, exhibited antiestrogenic activities at higher concentrations (>20 µM) and thus inhibited the proliferation of breast cancer cells [12,13].…”

Section: Introductionmentioning

confidence: 80%

Estrogenic and Antiestrogenic Activities of Commercial Dietary Supplements Containing Herbal Ingredients and Isoflavones

Basu

Sunny

Maier

2016

Int J Pharm Pharm Sci

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Objective: This study aimed to assess the estrogenic and antiestrogenic activities of five dietary supplements, commercially available for treatment of menopausal symptoms, before and after gastrointestinal digestion by employing a yeast steroid-regulated transcription system. Methods: Supplements (S) were extracted with either 80% methanol or water. Water extracts were subjected to simulated gastrointestinal (GI) digestion. Estrogenic and antiestrogenic activities were assessed by a steroid-regulated transcription system in Saccharomyces cerevisiae expressing the human estrogen receptor alpha. Results:The highest estrogenic activities were detected in both S1 methanol (2342.5±20.83 MU) and water (1225.6±20.6 MU) extracts (400 estradiol equivalents). Extracts showed antiestrogenic properties by reducing the transcriptional activity induced by estradiol in transgenic yeast. The highest antiestrogenic activity was detected in S2 methanol extract and S3 water extract, which inhibited estradiol activity by 76% and 64%, respectively. After GI digestion, S1, S2 and S3 extracts showed significantly higher estrogenic and antiestrogenic activities as 'serum-available' than 'colon-available' samples and S4 and S5 extracts showed significantly higher activities as 'colon-available' than 'serum-available' samples. Conclusion:All dietary supplements revealed estrogenic and antiestrogenic activities. The GI digestion demonstrated the availability of phytoestrogens for absorption in the blood stream. Supplements containing soy isoflavones and alfalfa ingredients had the highest estrogenic activities and could be more effective than supplements with complex plant formulation in alleviating menopausal symptoms and treating osteoporosis. The transgenic yeast assays proved to be a powerful tool for assessing the in vitro estrogenic and antiestrogenic activities of dietary supplements.

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“…Daidzein reported to possess strong anti-proliferative, anti-inflammatory and anti-fibrinolytic activity (Choi et al, 2013;Danyelle et al, 2008). Thus, in this study results of different evaluation such as visual score, histological analysis and hydroxyproline content suggested the potential effect of daidzein against the intra articular adhesion in post operative case.…”

Section: Discussionmentioning

confidence: 99%

Reduction of Intra-Articular Adhesion by Topical Application of Daidzein Following Knee Surgery in Rabbits

Liu

Jia

Hong-sheng

2017

Afr J Tradit Complement Altern Med

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Background: Intra-articular adhesion is the commonest complication that is faced by orthopedic surgeons after knee surgery. Thus, the present investigation evaluates the effect of daidzein on intra-articular adhesion in rabbits. Material and methods: All the rabbits were separated in to four different groups each group carries ten rabbits. Cancellous bone was exposed in each rabbit by removing cortical bone from both side of the femoral condyle. Following daidzein (2.5, 5 and 10 mg/ml) was topically applied for the duration of 10 min to the decorticated areas. Thereafter for the period of 4 week surgical limb was fixed. Effect of daidzein on intra articular adhesion was estimated by visual score through macroscopic examination, histopathology study, hydroxyproline content, fibroblast and collage density. Results: Data obtained in the study suggest that topical application of daidzein (5 and 10 mg/ml) loose the collagen and significantly decreases the adhesion at the decorticated areas. Moreover there were significant reduction in the fibroblast density, hydroxyproline content and optical density of collagen tissue in daidzein (5 and 10 mg/ml) treated group than control. Conclusion: Thus present study concludes that topical application of daidzein reduces intra-articular adhesion around the knee.

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Antiproliferative activity of daidzein and genistein may be related to ERα/c-erbB-2 expression in human breast cancer cells

Cited by 76 publications

References 26 publications

Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells

Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells

Estrogenic and Antiestrogenic Activities of Commercial Dietary Supplements Containing Herbal Ingredients and Isoflavones

Reduction of Intra-Articular Adhesion by Topical Application of Daidzein Following Knee Surgery in Rabbits

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