Antiproliferative and apoptosis inducing activity of Markhamia tomentosa leaf extract on HeLa cells

Ibrahim, Bolanle; Sowemimo, Abimbola; Spies, Luanne; Koekomoer, Trevor; Venter, Maryna van de; Odukoya, Onatolu

doi:10.1016/j.jep.2013.07.040

Cited by 37 publications

(30 citation statements)

References 15 publications

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“…Under normal conditions, the balance between proliferation and programmed cell death, which usually occurs via apoptosis, is maintained by regulating both processes to ensure the integrity of tissues and organs. However, uncontrolled cell division can induce tissue proliferation and even cancer [14]. Therefore, the inhibition of cell proliferation is thought to be an effective method for tumor therapy.…”

Section: Resultsmentioning

confidence: 99%

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

Pan

Zhao

et al. 2016

Marine Drugs

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In this study, the hexapeptide Phe-Ile-Met-Gly-Pro-Tyr (FIMGPY), which has a molecular weight of 726.9 Da, was separated from skate (Raja porosa) cartilage protein hydrolysate using ultrafiltration and chromatographic methods, and its anticancer activity was evaluated in HeLa cells. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay indicated that FIMGPY exhibited high, dose-dependent anti-proliferation activities in HeLa cells with an IC50 of 4.81 mg/mL. Acridine orange/ethidium bromide (AO/EB) fluorescence staining and flow cytometry methods confirmed that FIMGPY could inhibit HeLa cell proliferation by inducing apoptosis. Western blot assay revealed that the Bax/Bcl-2 ratio and relative intensity of caspase-3 in HeLa cells treated with 7-mg/mL FIMGPY were 2.63 and 1.83, respectively, significantly higher than those of the blank control (p < 0.01). Thus, FIMGPY could induce apoptosis by upregulating the Bax/Bcl-2 ratio and caspase-3 activation. Using a DNA ladder method further confirmed that the anti-proliferation activity of FIMGPY was attributable to its role in inducing apoptosis. These results suggest that FIMGPY from skate cartilage protein hydrolysate may have applications as functional foods and nutraceuticals for the treatment and prevention of cancer.

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Section: Resultsmentioning

confidence: 99%

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

Pan

Zhao

et al. 2016

Marine Drugs

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“…(33585/HNC)/EuphorbiaceaeInfertility, menstrual pain, dysentery, typhoid fever [12]Leaves, whole plantProstratins A, B and C, rugosins A, D, E and G, quercetin 3- O -β-sambubioside, euphorbins G and H, tellimagradin I and II, kaempferol, cosmosiin, rhamnetin-3-galactoside, quercetin, β-amyrine acetate, β-sitosterol, campesterol, stigmasterol and cholesterol [26, 27]Antibacterial, antifungal, bleeding haemorrhoids [12, 28, 29] Markhamia tomentosa K. Schum. (1974/SRF-Cam)/BignoniaceaeEdema, cancer, gout, scrotal elephantiasis, pulmonary troubles, general body pain [30]Leavesβ-sitosterol, β-sitosterol-3- O -β- d -glucopyranoside, dehydroiso-α-lapachone, 2-acetylnaphtho[2,3-b]furan-4,9-dione, pomolic acid, 2-acetyl-6-methoxynaphtho[2,3-b]furan-4,9-dione, β-lapachone, tormentic acid, oleanolic acid, palustrine [31, 32]Antiprotozoal, antihelmintic, antibacterial, antifungal, antiviral, analgesic, anti-inflammatory antioxidant, anti-tumor [31–33] Viscum album L. (2974/HNC)/LoranthaceaeAtherosclerosis, hypertension, cancer, headache, dizziness, palpitation [34, 35]LeavesConiferin, syringin, eleutheroside E, syringaresinol- O -glucoside, ligalbumosides A–E, alangilignoside C, kalopanaxin D, β-amyrin acetate, β-amyrin, lupeol, lupeol acetate oleanolic acid, betulinic acid, stigmasterol, β-sitosterol, trans -α-bergamotene, trans -β-farnesene, loliolide, vomifoliol, 5,7-dimethoxy-flavanone-4′- O -glucoside, 2′-hydroxy-4′,6′-dimethoxychalcone-4- O -glucoside, 5,7-dimethoxyflavanone-4′- O -[2′′- O -(5′′′- O - trans -cinnamoyl)-apiosyl]-glucoside), 2′-hydroxy-4′,6′-dimethoxychalcone-4- O -[2′′- O -(5′′′- O - trans -cinnamoyl)-apiosyl]-glucoside, 2′-hydroxy-3,4′,6′-trimethoxychalcone-4- O -glucoside, 2′-hydroxy-4′,6′-dimethoxychalcone-4- O -[apiosyl(1 → 2)]-glucoside, 5,7-dimethoxyflavanone-4′- O -[apiosyl-(1 → 2)]-glucoside, (2 S )-3′,5,7-trimethoxyflavanone-4′- O -glucoside, 4′- O -[β-apiosyl(1 → 2)]-β-glucosyl]-5-hydroxy-7- O -sinapylflavanone, 3-(4-acetoxy-3,5-dimethoxy)-phenyl-2 E -propenyl-β-glucoside, 3-(4-hydroxy-3,5-dimethoxy)-phenyl-2 E -propenyl-β-glucoside, 4′,5-dimethoxy-7-hydroxy flavanone, 5,7-dimethoxy-4′-hydroxy flavanone [36]Antihypertensive, cytotoxicity, vascular effects, antioxidant, antibacterial [34, 35, 37, 38] Rauwolfia macrophylla Ruiz & Pav. (1697/SRFK)/ApocynaceaeMeasles or itching rash, fever, swellings, rheumatism, hepatitis, pneumonia, abdominal pain, cough and toothache, headache, insomnia and palpitation of the heart, abscess, roundworm, tapeworm, hypertension, epilepsy, eye diseases, venereal diseases [39]Leaves, bark, roots…”

Section: Methodsmentioning

confidence: 99%

Multidrug resistant bacteria are sensitive to Euphorbia prostrata and six others Cameroonian medicinal plants extracts

2017

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BackgroundMultidrug resistant (MDR) bacteria are responsible for therapeutic failure and there is an urgent need for novels compounds efficient on them.MethodsEleven methanol extracts from seven Cameroonian medicinal plants were tested for their antibacterial activity using broth micro-dilution method against 36 MDR bacterial strains including Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aeruginosa and Staphylococcus aureus.Results Euphorbia prostrata extract was found active against all the 36 tested bacteria including Gram-negative phenotypes over-expressing efflux pumps such as P. aeruginosa PA124, E. aerogenes CM64 and E. coli AG102. E. prostrata had minimal inhibitory concentrations values between 128 and 256 µg/mL on 55.55% of the studied microorganisms. Other plants extract displayed selective antibacterial activity.ConclusionsResults obtained in this study highlight the antibacterial potential of the tested plants and the possible use of E. prostrata to combat bacterial infections including MDR phenotypes.

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“…K.Schumex.Engl. (Ibrahim et al, 2013), of Compositae, Echinops giganteus var. lelyi (C. D. Adams) A.…”

Section: Central Eastern and Western Africa Plants And Derived Molecmentioning

confidence: 99%

Potential of Central, Eastern and Western Africa Medicinal Plants for Cancer Therapy: Spotlight on Resistant Cells and Molecular Targets

Mbaveng

Kuete

Efferth³

2017

Front. Pharmacol.

113

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Cancer remains a major health hurdle worldwide and has moved from the third leading cause of death in the year 1990 to second place after cardiovascular disease since 2013. Chemotherapy is one of the most widely used treatment modes; however, its efficiency is limited due to the resistance of cancer cells to cytotoxic agents. The present overview deals with the potential of the flora of Central, Eastern and Western African (CEWA) regions as resource for anticancer drug discovery. It also reviews the molecular targets of phytochemicals of these plants such as ABC transporters, namely P-glycoprotein (P-gp), multi drug-resistance-related proteins (MRPs), breast cancer resistance protein (BCRP, ABCG2) as well as the epidermal growth factor receptor (EGFR/ErbB-1/HER1), human tumor suppressor protein p53, caspases, mitochondria, angiogenesis, and components of MAP kinase signaling pathways. Plants with the ability to preferentially kills resistant cancer cells were also reported. Data compiled in the present document were retrieved from scientific websites such as PubMed, Scopus, Sciencedirect, Web-of-Science, and Scholar Google. In summary, plant extracts from CEWA and isolated compounds thereof exert cytotoxic effects by several modes of action including caspases activation, alteration of mitochondrial membrane potential (MMP), induction of reactive oxygen species (ROS) in cancer cells and inhibition of angiogenesis. Ten strongest cytotoxic plants from CEWA recorded following in vitro screening assays are: Beilschmiedia acuta Kosterm, Echinops giganteus var. lelyi (C. D. Adams) A. Rich., Erythrina sigmoidea Hua (Fabaceae), Imperata cylindrical Beauv. var. koenigii Durand et Schinz, Nauclea pobeguinii (Pobég. ex Pellegr.) Merr. ex E.M.A., Piper capense L.f., Polyscias fulva (Hiern) Harms., Uapaca togoensis Pax., Vepris soyauxii Engl. and Xylopia aethiopica (Dunal) A. Rich. Prominent antiproliferative compounds include: isoquinoline alkaloid isotetrandrine (51), two benzophenones: guttiferone E (26) and isoxanthochymol (30), the isoflavonoid 6α-hydroxyphaseollidin (9), the naphthyl butenone guieranone A (25), two naphthoquinones: 2-acetylfuro-1,4-naphthoquinone (4) and plumbagin (37) and xanthone V1 (46). However, only few research activities in the African continent focus on cytotoxic drug discovery from botanicals. The present review is expected to stimulate further scientific efforts to better valorize the African flora.

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Antiproliferative and apoptosis inducing activity of Markhamia tomentosa leaf extract on HeLa cells

Cited by 37 publications

References 15 publications

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

Anticancer Activity of a Hexapeptide from Skate (Raja porosa) Cartilage Protein Hydrolysate in HeLa Cells

Multidrug resistant bacteria are sensitive to Euphorbia prostrata and six others Cameroonian medicinal plants extracts

Potential of Central, Eastern and Western Africa Medicinal Plants for Cancer Therapy: Spotlight on Resistant Cells and Molecular Targets

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