Antiproliferative, Antioxidative and Compounds Identification from Methanolic Extract of Passiflora Foetida and Its Fractions

Sul’ain, Mohd Dasuki; Sisin, Noor Nabilah Talik; Abdullah, Huda

doi:10.15406/japlr.2017.05.00166

Cited by 3 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, a Varioskan Flash microplate reader (Thermo Fisher) was used to read the fluorescence from the cells, with the 535 nm excitation and 615 nm of emission wavelengths to identify the viable cells [40][41][42]. Although the cell densities seeded earlier varied, the cell viabilities were compared to the respective control cells [43,44], according to Eq. 1.…”

Section: Synthesis Of Fe Solid Complexesmentioning

confidence: 99%

Analysis of Novel Schiff Base-Fe Complexes Against Breast Cancer Cells’ Viability

Sisin,

Algethami,

Assran

et al. 2023

KEM

View full text Add to dashboard Cite

Two potential novels Schiff base iron (Fe) complexes Fe-L2 and Fe-L3 (where L2= N, N'-bis (o-hydroxyacetophenone) ethylenediamine and L3= N, N'-bis (o-hydroxybenzaldehyde) phenylenediamine) were synthesized from interaction a hot methanolic solution of each ligand L2 or L3 (0.01mole) with the appropriate amount of Fe (NO3)3.9H2O metal salt (0.01mole). This study investigated the cytotoxicity induced by both complexes (0.1 to 100 µg/ml) in MCF-7 and MDA-MB 231 cell lines. After 24 hours of treatment, the cell viabilities of both MCF-7 and MDA-MB-231 cells were linearly proportional to the Fe-L2 concentrations. A higher concentration of Fe-L2 would cause higher cell killings. On the other hand, most of the Fe-L3 concentrations caused total cell deaths for both cell lines, except for the lowest concentration (0.1 µg/ml). Fe-L2 and Fe-L3 also caused lower cell viability of MDA-MB-231 cells compared to MCF-7 cells. Overall, the obtained Fe-L3 is more toxic than Fe-L2 in breast cancer cells. It is suggested that the Fe-L3 is an excellent agent against breast cancer cells; meanwhile, the Fe-L2 is biocompatible and a good support in medical applications, especially as a radiosensitizer in radiotherapy.

show abstract

Section: Synthesis Of Fe Solid Complexesmentioning

confidence: 99%

Analysis of Novel Schiff Base-Fe Complexes Against Breast Cancer Cells’ Viability

Sisin,

Algethami,

Assran

et al. 2023

KEM

View full text Add to dashboard Cite

show abstract

“…However, investigations of the antioxidant activity of the column chromatography fraction of P. foetida are still scarce. Research by Sisin et al 23 reported the antioxidant activity of the P. foetida whole-plant fraction, which used isocratic elution: petroleum ether and ethyl acetate in this study. Research has yet to be carried out to reveal the antioxidant activity of P. foetida leaves column chromatography fractions with gradient elution.…”

Section: Introductionmentioning

confidence: 99%

Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves

Triadisti¹,

Zamzani²

2023

Borneo J Pharm

View full text Add to dashboard Cite

Available synthetic antioxidants have been reported to have mutagenic and toxic effects. On the other hand, natural antioxidants show their superiority as they are not or less toxic. Passiflora foetida has the potential as an antioxidant, but the investigation of the antioxidant activity of the P. foetida chromatography column fraction has not been reported. This studied aims to investigate the antioxidant activity of the column chromatographic fractions of P. foetida leaves. An antioxidant assay using the DPPH and FRAP methods. The extraction was carried out by graded maceration, then fractionation using column chromatography. The antioxidant activity test was carried out using the DPPH and FRAP methods. Thin Layer Chromatography analysis was performed to determine the chromatogram pattern. The EC50 using DPPH method from n-hexane extract: 129.035 µg/mL, ethyl acetate extract: 206.398 µg/mL, methanol extract: 97.453 µg/mL, while the EC50 using FRAP method from n-hexane extract: 67.851 µg/mL, ethyl acetate extract : 68.981 µg/mL, and methanol extract: 58.787 µg/mL. Column chromatography fractions have antioxidant activity, with FMetPF6 as the fraction with the best activity, with percent inhibition 41.85±1.96 at concentration 25 µg/mL (DPPH), and with percent antioxidant activity 26.03±0.84 at concentration 9 µg/mL (FRAP). Passiflora foetida leaves have great potential as an antioxidant; both the extract and its fractions have antioxidant activity. The FMetPF6 has the best activity compare to other extracts and fractions. Further analysis to determine the various compounds in FMetPF6 using LC-MS/MS will facilitate the active compound's isolation.

show abstract

Hydrodistillation Optimization for Borago officinalis L. Essential Oil and Its Chemical Composition Analysis

Slama,

Slougui,

Ounnas

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

The essential oil extracted from Borago officinalis L. aerial parts using hydrodistillation contains several compounds, although it yields low amounts. For the first time, this study investigated the effects of hydrodistillation parameters and optimized conditions to maximize the yield of B. officinalis essential oil while characterizing its chemical composition using gas chromatography‐mass spectrometry. A single‐factor evaluation of three extraction parameters—extraction time, liquid‐to‐solid ratio, and soaking time—revealed optimal conditions of 4 h, 10:1 mL/g, and 2 h, respectively. Subsequently, the Box‐Behnken response surface method produced a significant polynomial model, determining optimal conditions of an extraction time of 5 h, a liquid‐to‐solid ratio of 11.06:1 mL/g, and a soaking time of 2 h, 17 min, and 34 s, resulting in an essential oil yield of 0.42%. 21 compounds were identified in B. officinalis essential oil, predominantly nonacosane (29.15%) and phytol (27.92%).

show abstract

Antiproliferative, Antioxidative and Compounds Identification from Methanolic Extract of Passiflora Foetida and Its Fractions

Cited by 3 publications

References 28 publications

Analysis of Novel Schiff Base-Fe Complexes Against Breast Cancer Cells’ Viability

Analysis of Novel Schiff Base-Fe Complexes Against Breast Cancer Cells’ Viability

Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves

Hydrodistillation Optimization for Borago officinalis L. Essential Oil and Its Chemical Composition Analysis

Contact Info

Product

Resources

About