Antiproliferative effect of Tualang honey on oral squamous cell carcinoma and osteosarcoma cell lines

Abstract

BackgroundThe treatment of oral squamous cell carcinomas (OSCC) and human osteosarcoma (HOS) includes surgery and/or radiotherapy which often lead to reduced quality of life. This study was aimed to study the antiproliferative activity of local honey (Tualang) on OSCC and HOS cell lines.MethodsSeveral concentrations of Tualang honey (1% - 20%) were applied on OSCC and HOS cell lines for 3, 6, 12, 24, 48 and 72 hours. Morphological characteristics were observed under light and fluorescent microscope. Cell viability was assessed using MTT assay and the optical density for absorbance values in each experiment was measured at 570 nm by an ELISA reader. Detection of cellular apoptosis was done using the Annexin V-FITC Apoptosis Detection Kit.ResultsMorphological appearance showed apoptotic cellular changes like becoming rounded, reduction in cell number, blebbed membrane and apoptotic nuclear changes like nuclear shrinkage, chromatin condensation and fragmented nucleus on OSCC and HOS cell lines. Cell viability assay showed a time and dose-dependent inhibitory effect of honey on both cell lines. The 50% inhibitory concentration (IC50) for OSCC and HOS cell lines was found to be 4% and 3.5% respectively. The maximum inhibition of cell growth of ≥80% was obtained at 15% for both cell lines. Early apoptosis was evident by flow cytometry where percentage of early apoptotic cells increased in dose and time dependent manner.ConclusionTualang honey showed antiproliferative effect on OSCC and HOS cell lines by inducing early apoptosis.

Impact of Fortification With Honey on Some Properties of Bio-Yoghurt

“…Besides its sugar composition, honey consists of a number of bioactive compounds such as phenolic compounds, flavonoids, carotenoid-like derivatives, organic acids, Maillard reaction products, catalase, ascorbic acid, and other compounds which function as antioxidants (Bogdanov et al, 2008). Several therapeutic and medicinal effects such as antibacterial, antimutagenic, antiproliferative, hepatoprotective, hypoglycemic, and antioxidant effects have been ascribed to honey through last years (Erejuwa et al, 2010 andGhashm et al, 2010). Poorani et al (2012) stated that honey, which is naturally available good product with high nutritive and medicinal value can be used preparing a bifidiogenic milk product by assessing the content of bifidus growth factor and further incorporation will give a valuable product.…”

Tualang Honey: Composition, Physiochemical Properties and Clinical Importance

“…Also it showed antiproliferative effect on oral squamous cell carcinomas and human osteo-sarcoma cell lines by inducing early apoptosis 14 . Tualang honey has a significant anticancer activity against breast cancer cells comparable to the effect of tamoxifen (TAM).…”

Improved spatial learning and memory performance following Tualang honey treatment during cerebral hypoperfusion-induced neurodegeneration

“…These antioxidant and radical scavenging effects are seen in all types of honeys. Nevertheless, these effects are in different proportions in different honey depending on the geographical areas, different floral source as well as climate [8]. Tualang honey is used commonly as a medicinal product as well as food in Malaysia.…”