Antiproliferative effects and molecular mechanisms of troglitazone in human cervical cancer in vitro

Abstract

We investigated the effects of troglitazone on human cervical cancer SiHa cells and its mechanisms of action. SiHa cells were incubated with different concentrations of troglitazone (100, 200, or 400 μg/mL) for 24, 48, and 72 hours. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay; cell cycle and apoptosis were detected by flow cytometry; and morphology of SiHa cells was observed under an inverted microscope. pcDNA3.1 and pcDNA3.1-Skp2 plasmids were constructed and then transfected into SiHa cells. Protein expression was analyzed by Western blotting. Troglitazone inhibited the proliferation of SiHa cells in a time- and concentration-dependent manner. Troglitazone caused G0/1 phase arrest but failed to reduce apoptosis in SiHa cells. Troglitazone significantly increased expression of p27 but decreased Skp2 expression. Skp2 overexpression inhibited the role of troglitazone in increasing expression of p27, and the cell cycle inhibitory effect of troglitazone. Troglitazone can inhibit SiHa cell viability by affecting cell cycle distribution but not apoptosis, and Skp2 and p27 may play a critical role.

Risk model in stage IB1-IIB cervical cancer with positive node after radical hysterectomy

“…Cervical carcinoma is one of the most common causes of cancer death in women across the world. 1 , 2 Experts estimated that there were 527,600 new patients and 265,700 deaths across the world in 2012. 3 In the USA, >12,000 women are diagnosed every year with invasive cervical carcinoma and >220,000 women are living with the disease.…”

Vitamin K3 induces antiproliferative effect in cervical epithelial cells transformed by HPV 16 (SiHa cells) through the increase in reactive oxygen species production

“…It is known that biological behavior of malignant tumor cells includes disorderly proliferation and distant metastasis [20]. Vitamin K3 induced cytotoxic effects in SiHa cells, inhibiting cell proliferation.…”

The effect of albumin and hemoglobin levels on the prognosis of early-stage cervical cancer: a prospective, single-center–based cohort study

“…Nutritional status is closely related to tumor inflammatory response [ 7 ]. Cancer-associated inflammatory mediators promote tumor growth, invasion, metastasis, and angiogenesis [ 8 ]. Anemia and hypoalbuminemia affect the immune tolerance environment of tumor tissues and can activate proinflammatory-related pathways, thereby affecting the occurrence and development of tumors and patients’ prognosis [ 9 ].…”