Antiproliferative Evaluation of Isofuranodiene on Breast and Prostate Cancer Cell Lines

Abstract

The anticancer activity of isofuranodiene, extracted from Smyrnium olusatrum, was evaluated in human breast adenocarcinomas MDA-MB 231 and BT 474, and Caucasian prostate adenocarcinoma PC 3 cell lines by MTS assay. MTS assay showed a dose-dependent growth inhibition in the tumor cell lines after isofuranodiene treatment. The best antiproliferative activity of the isofuranodiene was found on PC 3 cells with an IC50 value of 29 μM, which was slightly less than the inhibition against the two breast adenocarcinoma cell lines with IC50 values of 59 and 55 μM on MDA-MB 231 and BT 474, respectively. Hoechst 33258 assay was performed in order to study the growth inhibition mechanism in prostate cancer cell line; the results indicate that isofuranodiene induces apoptosis. Overall, the understudy compound has a good anticancer activity especially towards the PC 3. On the contrary, it is less active on Chinese hamster ovary cells (CHO) and human embryonic kidney (HEK 293) appearing as a good candidate as a potential natural anticancer drug with low side effects.

A halogen bond-mediated highly active artificial chloride channel with high anticancer activity

“…For comparison, highly effective anticancer agent cisplatin has an IC 50 value of 37 μM against the same BT-474 cells. 13 Assuming all A10 molecules associate to form channels with each channel comprising six or more such molecules, the IC 50 value in terms of effective channel concentration is lower than 3.3 μM.…”

Blue honeysuckle fruit (Lonicera caerulea L.) from eastern Russia: phenolic composition, nutritional value and biological activities of its polar extracts

“…Human fibroblasts cells were cultured in Minimum Essential Medium containing 15% fetal bovine serum (FBS), 2mM Lglutamine, 100 U mL -1 penicillin and 100 µg mL -1 streptomycin and incubated at 5% CO 2 at 37° C. Blue honeysuckle fruit aqueous extract was dissolved in water at a concentration of 10 mg mL . 20 After the addiction of MTS, in combination with the electron coupling agent phenazine methosulfate, the cells were allowed to incubate for 1 h and absorbance was measured at 492 nm in a microplate reader, GeniosPro. Cell viability was calculated as a percentage using the formula: (mean OD of treated cells/mean OD of control cells) x 100.…”

Mechanistic Pathways and Molecular Targets of Plant-Derived Anticancer ent-Kaurane Diterpenes

“…In addition, a large number of cancer patients have forms of the disease that are resistant to the mainstay anticancer drugs. Currently used anticancer drugs are mainly obtained from natural sources (plants, microorganisms, and marine organisms) or from synthetic reactions [3]. Unfortunately, most of these drugs also kill normal cells generating severe side effects [4,5].…”