Antipsoriatic activity and cytotoxicity of ethanolic extract of Nigella sativa seeds

Abstract

Background:Nigella sativa Linn (Ranunculaceae) is popularly known as black cumin with a wide spectrum of pharmacological activities including anti-inflammatory, antibacterial, antifungal and antihelmenthic. The seeds are externally applied for eruptions of skin. The seeds are used traditionally for psoriasis tropicus with general pain and eruption of patches.Objective:The ethanolic extract of Nigella sativa seeds were evaluated for antipsoriatic activity.Materials and Methods:The screening of antipsoriatic activity of 95% of ethanolic extract of Nigella sativa seeds by using mouse tail model for psoriasis and in vitro antipsoriatic activity was carried out by SRB Assay using HaCaT human keratinocyte cell lines.Results:The ethanolic extract of Nigella sativa seeds extract produced a significant epidermal differentiation, from its degree of orthokeratosis (71.36±2.64) when compared to the negative control (17.30±4.09%). This was equivalent to the effect of the standard positive control, tazarotene (0.1%) gel, which showed a (90.03±2.00%) degree of orthokeratosis. The 95% ethanolic extract of Nigella sativa shown IC50 239 μg/ml, with good antiproliferant activity compared to Asiaticoside as positive control which showed potent activity with IC50 value of 20.13 μg/ml.Conclusion:The ethanolic extract of Nigella sativa seeds also showed increase in relative epidermal thickness when compared to control group by confirming its traditional use in psoriasis treatment.

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“…sativa widely use evidence showed its anti-inflammatory effect is comparable effect of of 100 mg/kg aspirin by its inhibitory effects on Carrageenan induced paw edema [28]. Also, evidences show N. sativa anti-inflammatory therapeutic effects on psoriasis (by produced the ethanolic extract) [29], Acne vulgaris (as an oil lotion 10% significantly reduced mean lesion count of and pustules after 2 months of therapy) [30, 31], Vitiligo (by Lyophilized seed extract of N. sativa and its active ingredient) [32, 33], Hypersensitivity reactions (by using carbonyl fraction of N. sativa and its active components, thymoquinone and nigellone) [34, 35] and Skin cancers (by enhancement of the natural killer (NK) cell in anti-cancer program) [36, 37].…”

Kedi Baş ve Boyun Dermatitisi İçin Topikal Curcuma longa ve Nigella sativa Kombinasyonunun Etkinliği: Açık Pilot Çalışma

“…Turmeric attenuates the inflammatory response by down regulating the activities of nitric oxide synthase, lipoxygenase, and cyclooxygenase-2 [8] . On the other hand, black cumin might possess immune-stimulatory, anti-inflammatory [9] , and antiallergic [10] effects. Turmeric and black cumin caused complete recovery and totally decreased pruritus scores in this study.…”

Can Essential Oils/Botanical Agents Smart-Nanoformulations Be the Winning Cards against Psoriasis?

“…The study showed that orthokeratosis was five times higher after topical application of the ethanolic extract when compared with a placebo control, which was nearly equivalent to the positive control tazarotene (0.1%) gel. The antiproliferant activity of the ethanolic extract of Nigella sativa was accepted and comparable to that of the positive control, asiaticoside [ 66 ].…”