Antiquorum Sensing and Antibiofilm Potential of Capparis spinosa

Packiavathy, Issac Abraham Sybiya Vasantha; Agilandeswari, Palani; Ramaswamy, Babu Rajendran; Shunmugiah, Karutha Pandian; Ravi, Arumugam Veera

doi:10.1016/j.arcmed.2011.12.002

Cited by 163 publications

(103 citation statements)

References 40 publications

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“…Quantification of precipitated EPS revealed 30.64 % reduction in the EPS production in K. pneumoniae treated with 50 μg/ml of cyanidin compared to control (P < 0.05). Our results are in accordance with Abraham et al (2011) reported that Capparis spinosa reduced EPS production up to 67 % in P. mirabilis.…”

Section: Reduction In Eps Productionsupporting

confidence: 83%

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Gopu

Shetty

2016

J Food Sci Technol

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Quorum sensing (QS) is the process of population dependent cell to cell communication used by bacteria to regulate their phenotypic characteristics. Key virulence factors that determine the bacterial pathogenicity and food spoilage were found to be regulated by QS mechanism. Hence, disrupting the QS signaling pathway could be an attractive strategy to manage food borne pathogens. In the current study, QS inhibitory activity of a naturally occurring anthocyanincyanidin and its anti-biofilm property were assessed against an opportunistic pathogen Klebsiella pneumoniae, using a biosensor strain. Further, QS inhibitory property of a naturally occurring anthocyanin cyanidin was further confirmed using in-silico techniques like molecular docking and molecular dynamics simulation studies. Cyanidin at sub-lethal dose significantly inhibited QS-dependent phenotypes like violacein production (73.96 %), biofilm formation (72.43 %), and exopolysaccharide production (68.65) in a concentrationdependent manner. Cyanidin enhanced the sensitivity of test pathogen to conventional antibiotics in a synergistic manner. Molecular docking analysis revealed that cyanidin binds more rigidly with LasR receptor protein than the signaling compound with a docking score of −9.13 Kcal/mol. Molecular dynamics simulation predicted that QS inhibitory activity occurs through the conformational changes between the receptor and cyanidin complex. Our results indicate that cyanidin, can be a potential QS based antibiofilm and antibacterial agent for food borne pathogens.

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Section: Reduction In Eps Productionsupporting

confidence: 83%

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Gopu

Shetty

2016

J Food Sci Technol

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“…Some studies have shown that plant phenolic compounds can interfere with bacterial quorum sensing (Borges et al, 2014;Issac Abraham et al, 2011). Phenolic compounds are secondary metabolites found in plants and plant derived foods such as fruits, teas and red wine (Haminiuk et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

et al. 2016

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The aim of this study was to assess the anti-quorum sensing activity of phenolic extracts from grumixama (Eugenia brasiliensis), also known as Brazilian cherry, in concentrations that did not interfere with bacterial growth. The pulp phenolic compounds were extracted by using solid phage extraction in a mini-collumn C18 and quantified by spectrophotometry. The anti-quorum sensing activity was evaluated by testing the inhibition of violacein production in Chromobacterium violaceum and by evaluating the swarming motility in Aeromonas hydrophila and Serratia marcescens, both phenotypes regulated by quorum sensing. The phenolic extract strongly inhibited the production of violacein in C. violaceum, reducing its production in comparison with a control with no extract. No inhibition of growth was observed at the concentrations tested for quorum sensing inhibition. Confirming the quorum sensing inhibition phenotype, the extract was also able to inhibit swarming motility in S. marcescens and in A. hydrophila, although in the later the effect was marginal. Dverall, these results indicate that phenolic extract from E. brasiliensis presents quorum sensing inhibitory activity most likely due to the presence of fruit phenolics which have been implicated as quorum sensing inhibitors in Gram negative bacteria.Keywords: quorum sensing; anti-quorum sensing; phenolic extract; Brazilian cherry; Eugenia brasiliensis. Practical Application:The phenolic extract in this study presents potential applications in the food and pharmaceutical industries due to inhibition of phenotypes regulated by quorum sensing, which include production of virulence factors and spoilage enzymes. By inhibiting phenotypes associated with quorum sensing, the extract presents potential application as a natural preservative for food conservation and as an alternative or an adjuvant with antibiotics.

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“…Similar results were reported by Issac Abraham et al concerning Capparis spinosa (caper bush) extract. At a concentration of 2 mg/mL, an inhibition of E. coli bio�lm formation by 73% was observed [72]. For the pathogens Serratia marcescens, P. aeruginosa, and P. mirabilis, bio�lm biomass was reduced by 79%, 75%, and 70%, respectively.…”

Section: Plant Extractsmentioning

confidence: 79%

“…Moreover, the mature bio�lm structure was disrupted for all of the studied pathogens. Furthermore, the addition of C. spinosa extract (100 g/mL) to a bacterial culture resulted in swimming and swarming inhibition [72]. For Lagerstroemia speciosa (giant crape myrtle) extract, 83% bio�lm inhibition was achieved at a concentration of 10 mg/ mL [73].…”

Section: Plant Extractsmentioning

confidence: 93%

Antimicrobial, Antimycobacterial and Antibiofi lm Properties of Couroupita guianensis Aubl. Fruit Extract

Sayen¹

2014

Biofilm Control and Antimicrobial Agents

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We review the recent literature concerning the efficiency of antimicrobial photodynamic inactivation toward various microbial species in planktonic and bio�lm cultures. e review is mainly focused on bio�lm-growing microrganisms because this form of growth poses a threat to chronically infected or immunocompromised patients and is difficult to eradicate from medical devices. We discuss the bio�lm formation process and mechanisms of its increased resistance to various antimicrobials. We present, based on data in the literature, strategies for overcoming the problem of bio�lm resistance. �actors that have potential for use in increasing the efficiency of the killing of bio�lm-forming bacteria include plant extracts, enzymes that disturb the bio�lm structure, and other nonenzymatic molecules. We propose combining antimicrobial photodynamic therapy with various antimicrobial and antibio�lm approaches to obtain a synergistic effect to permit efficient microbial growth control at low photosensitizer doses.

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Antiquorum Sensing and Antibiofilm Potential of Capparis spinosa

Cited by 163 publications

References 40 publications

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Cyanidin inhibits quorum signalling pathway of a food borne opportunistic pathogen

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

Antimicrobial, Antimycobacterial and Antibiofi lm Properties of Couroupita guianensis Aubl. Fruit Extract

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