Antistress and anti-aging activities of Caenorhabditis elegans were enhanced by Momordica saponin extract

Lin, Chunxiu; Lin, Yizi; Wang, Xuebei; Hu, Lanyun; Cao, Yong; Chen, Yunjiao

doi:10.1007/s00394-020-02338-6

Cited by 33 publications

(29 citation statements)

References 57 publications

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“…A recent study showed that HSP16.2 could suppress Aβ toxicity by blocking its oligomerization, subsequently preventing Aβ-induced paralysis in transgenic C. elegans [ 52 ]. The activation of hsp16.1 and hsp16.2 genes by saponin derived from Mormodica exhibited antistress and antiaging properties [ 53 ]. To decrease α-synuclein aggregation, FA may activate the HSF-1 transcription factor, thereby activating it downstream, such as the hsp16.1 and hsp16 .2 genes we detected in the present study.…”

Section: Discussionmentioning

confidence: 99%

Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease

et al. 2021

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Parkinson’s disease (PD) is a currently incurable neurodegenerative disorder characterized by the loss of dopaminergic (DAergic) neurons in the substantia nigra pars compacta and α-synuclein aggregation. Accumulated evidence indicates that the saponins, especially from ginseng, have neuroprotective effects against neurodegenerative disorders. Interestingly, saponin can also be found in marine organisms such as the sea cucumber, but little is known about its effect in neurodegenerative disease, including PD. In this study, we investigated the anti-Parkinson effects of frondoside A (FA) from Cucumaria frondosa and ginsenoside Rg3 (Rg3) from Panax notoginseng in C. elegans PD model. Both saponins were tested for toxicity and optimal concentration by food clearance assay and used to treat 6-OHDA-induced BZ555 and transgenic α-synuclein NL5901 strains in C. elegans. Treatment with FA and Rg3 significantly attenuated DAergic neurodegeneration induced by 6-OHDA in BZ555 strain, improved basal slowing rate, and prolonged lifespan in the 6-OHDA-induced wild-type strain with downregulation of the apoptosis mediators, egl-1 and ced-3, and upregulation of sod-3 and cat-2. Interestingly, only FA reduced α-synuclein aggregation, rescued lifespan in NL5901, and upregulated the protein degradation regulators, including ubh-4, hsf-1, hsp-16.1 and hsp-16.2. This study indicates that both FA and Rg3 possess beneficial effects in rescuing DAergic neurodegeneration in the 6-OHDA-induced C. elegans model through suppressing apoptosis mediators and stimulating antioxidant enzymes. In addition, FA could attenuate α-synuclein aggregation through the protein degradation process.

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Section: Discussionmentioning

confidence: 99%

Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease

et al. 2021

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“…We observed that NHD improved the activities of SOD and GSH‐Px antioxidant enzymes under stress in nematodes. As shown by Momordica Saponin extract [36] . NHD might eliminate ROS directly or indirectly by activating the antioxidant system, thereby improving the healthspan and extending the lifespan of C. elegans .…”

Section: Discussionmentioning

confidence: 98%

Phytochemical Composition and Anti‐Aging Activity of Butanol Extract of Hedyotis diffusa in Caenorhabditis elegans

Liu

Dan-qing

et al. 2022

Chemistry & Biodiversity

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Hedyotis diffusa Willd. (H. diffusa), a kind of traditional Chinese medicine, has been evaluated to potential display antioxidant and anti‐aging effects in vitro experiments. In this work, we investigated the effects on lifespan and stress resistance of the butanol extract from H. diffusa (NHD) in vivo using a Caenorhabditis elegans (C. elegans) model. The phytochemicals of NHD were identified by UPLC‐ESI‐qTOF‐MS/MS method. NHD‐treated wild‐type N2 worms showed an increase in survival time under both normal and stress conditions. Meanwhile, NHD promoted the healthspan of nematodes by stimulating growth and development, reducing the deposition of age pigment, increasing the activities of superoxide dismutase (SOD) and glutathione peroxidase dismutase (GSH‐Px), and decreasing the level of ROS without impairing fertility. Moreover, the upregulating of the expression of daf‐16, gst‐4, sod‐3, hsp12.6 genes and the downregulating of the expression of daf‐2 were involved in the NHD‐mediated lifespan extension. Finally, the increasing of the expression of GST‐4::GFP in CL2166 transgenic nematodes and the life‐span‐extending activity of NHD was completely abolished in daf‐2 and daf‐16 mutants further revealed that the potential roles for these genes in NHD‐induced longevity in C. elegans. Collectively, our findings suggest that NHD may have an active effect in healthy aging and age‐related diseases.

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“…7,33 In our previous studies, we also found the benefits of MSs and CPPs in alleviating fat deposition and oxidative stress in C. elegans. [13][14][15][16] Therefore, the reason why MC could be used as a promising innovative functional beverage for antioxidant and anti-obesity could be due to MSs and CPPs, the main health-promoting active ingredients in M. charantia and C. paliurus leaves respectively. 6,10 We speculated that MC, a nontoxic and low toxicity innovative functional beverage, could promote the nuclear localization of DAF-16 and the translation of SOD-3 to activate the antioxidant system, and then to achieve the beneficial property of a reduction in oxidative stress (decreasing the level of ROS, MDA, and NEFAs).…”

Section: Wileyonlinelibrarycom/jsfamentioning

confidence: 99%

“…11 Interestingly, we also noted the potential of CPPs and MSs in relieving fat deposition and improving resistance in C. elegans. [13][14][15][16] Therefore, MSs may be a dietary supplement to treat obesity and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

“…According to our research, the taste of CPPs coordinates well with the bitterness of MSs. With the promising results in the antioxidant activity and the antiobesity activity of CPPs and MSs, [13][14][15][16] an innovative beverage based on these two extracts was developed (patent pending). First, to assess the antioxidant properties of the MSs-and CPPscontaining beverage (MC), the accumulation of oxidation byproducts and damage was investigated in C. elegans.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Momordica saponin‑ and Cyclocarya paliurus polysaccharide‐enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans

Lin

Xiao

et al. 2020

J Sci Food Agric

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BACKGROUND: As an edible and medicinal herb in Chinese folk medicine, Cyclocarya paliurus (Batal.) Iljinskaja leaves are traditionally widely used in the treatment of metabolic disorders. The vegetable Momordica charantia L. has been consumed worldwide for thousands of years as a traditional drug due to its activities against obesity and diabetes. In view of the therapeutic value of Momordica saponins (MSs) and C. paliurus polysaccharides (CPPs), an independently developed MSs-and CPPs-containing beverage (MC) was evaluated for its efficacy in controlling oxidative stress and obesity in Caenorhabditis elegans.RESULTS: First, we found that MC could promote the nuclear localization of DAF-16 and the translation of SOD-3. Further exploring its antioxidant properties, the oxidative stress by-products reactive oxygen species, malondialdehyde, and nonesterified fatty acids were significantly inhibited in C. elegans. Moreover, damage due to diseases related to oxidative stress (age pigments and neurodegenerative diseases) was alleviated. Furthermore, fat accumulation was significantly reduced in normal and high-fat models. Finally, the lipid-lowering effects of MC might involve reductions in the size and number of lipid droplets without impairing basic physiological functions in C. elegans.CONCLUSION: These results provide promising data indicating MC as an innovative health beverage for the pharmacological management of oxidative stress and obesity.

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Antistress and anti-aging activities of Caenorhabditis elegans were enhanced by Momordica saponin extract

Cited by 33 publications

References 57 publications

Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease

Neurorescue Effects of Frondoside A and Ginsenoside Rg3 in C. elegans Model of Parkinson’s Disease

Phytochemical Composition and Anti‐Aging Activity of Butanol Extract of Hedyotis diffusa in Caenorhabditis elegans

Effect of Momordica saponin‑ and Cyclocarya paliurus polysaccharide‐enriched beverages on oxidative stress and fat accumulation in Caenorhabditis elegans

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