Antithrombotic effect and mechanism of Rubus spp. Blackberry

Abstract

The compounds of Rubus spp. Blackberry (RSB) were isolated and identified by a bioassay-guided method, and their antithrombotic effects and mechanism were investigated with the acute blood stasis rat model. The RSB extract was evaluated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) assays in vitro. Results indicated that RSB extract exhibited anticoagulant activity. In addition to compounds 1 and 6, the other compounds also exhibited anticoagulant activity in vitro. Therefore, the in vivo antithrombosis effects of RSB extract were investigated by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of thromboxane B2 (TXB), 6-keto prostaglandin F1α (6-keto-PGF1α), endothelial nitric oxide synthase (eNOS) and ET-1 (endothelin-1) were measured. Results suggested that RSB extract had inhibitory effects on thrombus formation, and its antithrombotic effects were associated with the regulation of vascular endothelium active substance, activation of blood flow and an anticoagulation effect.

Phenolic contents-based assessment of therapeutic potential of Syzygium cumini leaves extract

“…Antidepressant drugs used as pain reliever in the cure of various chronic diseases have been traditionally obtained from plant based compounds [13]. There is a crucial need for active antithrombotic medicines to combat thrombotic diseases especially heart disease and cerebrovascular thrombosis [14]. Therefore, plant extracts can be used as alternative therapeutic agents for various medical issues due to the presence of diverse range of phytochemicals in them.…”

Isolation, purification, structural analysis and coagulatory activity of water-soluble polysaccharides from Ligustrum lucidum Ait flowers

“…These results showed that LLp-1a could benefit hindering fibrin formation. LLp-1b could significantly shorten APTT and increased FIB content, which indicated that its effects were mediated mainly through the intrinsic coagulation pathway and increasing the content of FIB [ 15 ].…”

“…Thrombosis involves local blood clotting of the vascular system that often leads to serious health-related diseases such as heart attacks and strokes. The risk factors for thrombosis are abnormal hyperlipid, hyperglycemia, elevated plasma fibrinogen, high blood pressure and cancer, these thrombotic diseases, have become the primary causes of death and their incidence has been increasing annually [ 14 , 15 ]. Therefore, effective antithrombotic drugs are urgently needed.…”

Antithrombotic Effect of Artemisia princeps Pampanini Extracts in Vitro and in FeCl3‐Induced Thrombosis Rats

“…Thrombosis is the formation of thrombus in the vascular system as a result of adhesion of activated platelets and blood coagulation (Xie, Cui, Shan, & Kang, ). The triggering of cardiovascular disease by thrombosis because of the activation of hemostasis and hypercoagulability is a major cause of deaths annually (Xie, Zhang, Wang, Wei, & Kang, ). The activation of coagulation leads to the formation and progression of arterial thrombus (Foley et al., ).…”