Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface

Cucurbitacin B (CuB), a triterpenoid compound isolated from Cucurbitaceae plants, has been reported as a promising anti-cancer agent, yet its action mechanism is still controversial. In this study, we explored the potential mechanism of CuB in murine B16F10 melanoma cells. Anti-proliferation and anti-invasion effects were assessed in cultured cells, and in vivo anti-tumor activity was evaluated in a murine subcutaneous melanoma model. Flow cytometry was adopted to analyze cell cycle distribution and reactive oxygen species (ROS) levels. Actin levels were determined by western blot analysis, and the profiles of differential expressed proteins were identified by a quantitative proteomic approach. The results showed that CuB exerted inhibitory effects on cell proliferation, colony formation, as well as migration and invasion potential of the melanoma cells. The growth of subcutaneous melanoma was significantly inhibited in mice treated with CuB when compared with control group. Furthermore, CuB treatment caused rapid cell membrane blebbing and deformation, and induced G 2 /M-phase arrest and formation of multiploid cells. Notably, the G-actin pool was rapidly depleted and actin aggregates were formed quickly after CuB treatment. A number of cytoskeleton-regulatory proteins were differentially regulated. Blockage of ROS production significantly reduced the G-actin depletion ability and the anti-tumor activity of CuB. These findings indicate that CuB induces rapid depletion of the G-actin pool through ROS-dependent actin aggregation in melanoma cells, which may at least partly account for its anti-tumor activity.

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“…, where 'A' represent the largest dimension of the tumor and 'B' indicates the smallest dimension [21].…”

Section: Measurement Of Cell Proliferation and Viabilitymentioning

confidence: 99%

Cucurbitacin B induces rapid depletion of the G-actin pool through reactive oxygen species-dependent actin aggregation in melanoma cells

Zhang

Ouyang

et al. 2011

ABBS

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“…In addition, they suppressed tumor metastasis. 12 Marker Ki67 is a nuclear protein found in the G1-phase of cell cycle and its expression is strictly associated with cell proliferation. The Ki67 antigen is a useful non-histonic protein, used to identify prolific cells that, not having phase specificity.…”

mentioning

confidence: 99%

CD105/Ki67 Coexpression Correlates With Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

Liu

Sun

et al. 2012

International Journal of Gynecological Cancer

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“…Ren et al[116] found that displaying the appropriate peptide on the T4 capsid could stimulate an immune response. They, previously had shown that the separate respective genetic modification of SOC and HOC with the polyprotein and proteinase of foot-and-mouth disease virus (FMDV) produced 100% immunogenicity compared to the wild type of T4[117].…”

Section: Bacteriophages As Nanocarriersmentioning

confidence: 99%

Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos

Karimi

Mirshekari

Basri

et al. 2016

Advanced Drug Delivery Reviews

154

108

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The main goal of drug delivery systems is to target therapeutic cargoes to desired cells and to ensure their efficient uptake. Recently a number of studies have focused on designing bio-inspired nanocarriers, such as bacteriophages, and synthetic carriers based on the bacteriophage structure Bacteriophages are viruses that specifically recognize their bacterial hosts. They can replicate only inside their host cell and can act as natural gene carriers. Each type of phage has a particular shape, a different capacity for loading cargo, a specific production time, and their own mechanisms of supramolecular assembly, that have enabled them to act as tunable carriers. New phage-based technologies have led to the construction of different peptide libraries, and recognition abilities provided by novel targeting ligands. Phage hybridization with non-organic compounds introduces new properties to phages and could be a suitable strategy for construction of bio-inorganic carriers. In this review we try to cover the major phage species that have been used in drug and gene delivery systems, and the biological application of phages as novel targeting ligands and targeted therapeutics.

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Antitumor activity of endogenous mFlt4 displayed on a T4 phage nanoparticle surface

Cited by 23 publications

References 29 publications

Cucurbitacin B induces rapid depletion of the G-actin pool through reactive oxygen species-dependent actin aggregation in melanoma cells

Cucurbitacin B induces rapid depletion of the G-actin pool through reactive oxygen species-dependent actin aggregation in melanoma cells

CD105/Ki67 Coexpression Correlates With Tumor Progression and Poor Prognosis in Epithelial Ovarian Cancer

Bacteriophages and phage-inspired nanocarriers for targeted delivery of therapeutic cargos

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