Antitumor activity of L-asparaginase from Yersinia pseudotuberculosis

Abakumova, O. Yu.; Podobed, O. V.; Borisova, Anna A.; Sidoruk, K. V.; Alexandrova, S.; Omelyanuk, N. M.; Pokrovskaya, M. V.; Kondakova, L. I.; Соколов, Н. Н.

doi:10.1134/s1990750809020115

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…The enzyme inhibited the growth of HL-60 cell line with IC 50 value of 0.14±0.03 U/ml. These results concur with those reported by Abakumova et al (2009) who evaluated the cytotoxic activity of recombinant L-ASNase from Yersinia pseudotuberculosis using MTT assay and recorded IC 50 value of 0.2 U/ml. By comparing effectiveness of recombinant L-ASNases from various sources one can observe that L-ASNase from E. coli MG27 exhibits higher activity in suppression of human myeloid leukemia cell line (HL-60) than the enzyme from Helicobacter pylori CCUG 17874 reported by Cappelletti et al (2008).…”

Section: Discussionsupporting

confidence: 91%

Expression, Purification and Characterization of Recombinant Histidine-tagged L-asparaginase II

2016

Egypt. J. Bot.

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The amino acid sequences of both are rather dissimilar except for a few regions of significant homology. The sequence corresponding to the mature (ansB) was subcloned into pQE-30 expression vector and expressed in E. coli M15. The recombinant histidine-tagged (ansB) was purified to homogeneity by Ni-NTA affinity chromatography and displayed a single 36.0 kDa band on SDS-PAGE. Results revealed that the recombinant His-tagged L-ASNase II was expressed in an active form and its specific activity was estimated to be 286 U/mg. The optimum temperature was attained at 40°C. The enzyme was maximum at pH 7-8. Also the enzyme was stable at 40-50°C. The purified functional enzyme exhibited a specific activity at 286 U/mg and inhibited the growth of human myeloid leukemia cell line (HL-60) with IC 50 value of 0.14±0.03 U/ml.

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Section: Discussionsupporting

confidence: 91%

Expression, Purification and Characterization of Recombinant Histidine-tagged L-asparaginase II

2016

Egypt. J. Bot.

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“…which is resemble to that of the enzymes from E. coil enzyme (pI =5.0) .different for the Erwinia(8.7). Recently, Abakumova et al [31] reported that the pI of L-asparaginase isolated from Yersinia pseudotuberculosis was 5.4.…”

Section: Citationmentioning

confidence: 99%

Isolation and Purification of High Efficiency L-asparaginase by Quantitative Preparative Continuous-elution SDS PAGE Electrophoresis

M¹,

Selvam²

2011

J Microbial Biochem Technol

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An Unique extracelluar glutaminase free L-asparaginase from novel marine Actinomycetes was isolated to perceptible homogeneity in agro industrial wastes. Quantitative Preparative Continuous-Elution SDS PAGE Electrophoresis is a high-resolution method for the preparative isolation of L-Asparaginase in biological samples. The enzyme was purified 248.68-fold and showed a final specific activity of 5035.28 IU/mg with an 80.71% yield. The homotetramer enzyme has a molecular mass of 133.25 kDa and an isoelectric point of approximately 5.4.Kinetic parameters, Km and Vmax of purified L-asparaginase from Streptomyces radiopugnans MS1 were found to be 0.0598, 3.5478 IU µg -1 respectively. The de novo sequencing strategy presented here provides a rapid and reliable means to identify proteins in Streptomyces radiopugnans MS1. The purified L-asparaginase has no glutaminase activity, which can diminish the leeway of side effects during the itinerary of anti-malignancy therapy.

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“…Цитотоксичность оксованадиевых соединений измеряли, используя МТТ-тест [25], который основан на способности митохондриальных дегидрогеназ только живых клеток образовывать кристаллы формазана из МТТ-реактива (3-[4,5-диметилтиа-2-ил]-2,5-дифенилтетразолий бромида), что позволяет определять количество метаболически активных живых клеток. Для определения цитотоксичности ванадильных комплексов клетки пассировали в 48-луночные планшеты в объёме 300 мкл.…”

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Anticancer activity of oxovanadium compounds

et al. 2013

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Cytotoxic and antitumor activity of the biligand vanadyl derivative of L-malic acid (bis(L-malato)oxovanadium(IV) (VO(mal) ) was investigated in comparison with inorganic vanadium(IV) compound - vanadyl sulfate (VOSO ) and also with oxovanadium monocomplex with L-malic acid (VO(mal)) and vanadyl biscomplex with acetylacetonate. In this purpose the effect of vanadyl compounds on growth of normal human skin fibroblasts and tumor cells of different lines: mouse fibrosarcoma (L929), rat pheochromocytome (PC12), human liver carcinoma (HepG2), virus transformated mouse fibroblast (NIN 3T3), virus transformated cells of human kidney (293) were investigated. The results showed that VO(mal) was not toxic for normal human skin fibroblasts but considerably inhibited growth of cancer cells in culture. Cytotoxic antitumor effect of vanadium complexes was found to be dependent оn incubation time and concentration and on type of cells and nature of ligands of the central group of the complex (VO2+). These studies provide evidence that VO(mal) may be considered as a potential antitumor agent due to its low toxicity in non-tumor cells and significant anticancer activity.

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Antitumor activity of L-asparaginase from Yersinia pseudotuberculosis

Cited by 6 publications

References 13 publications

Expression, Purification and Characterization of Recombinant Histidine-tagged L-asparaginase II

Expression, Purification and Characterization of Recombinant Histidine-tagged L-asparaginase II

Isolation and Purification of High Efficiency L-asparaginase by Quantitative Preparative Continuous-elution SDS PAGE Electrophoresis

Anticancer activity of oxovanadium compounds

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