An Unique extracelluar glutaminase free L-asparaginase from novel marine Actinomycetes was isolated to perceptible homogeneity in agro industrial wastes. Quantitative Preparative Continuous-Elution SDS PAGE Electrophoresis is a high-resolution method for the preparative isolation of L-Asparaginase in biological samples. The enzyme was purified 248.68-fold and showed a final specific activity of 5035.28 IU/mg with an 80.71% yield. The homotetramer enzyme has a molecular mass of 133.25 kDa and an isoelectric point of approximately 5.4.Kinetic parameters, Km and Vmax of purified L-asparaginase from Streptomyces radiopugnans MS1 were found to be 0.0598, 3.5478 IU µg -1 respectively. The de novo sequencing strategy presented here provides a rapid and reliable means to identify proteins in Streptomyces radiopugnans MS1. The purified L-asparaginase has no glutaminase activity, which can diminish the leeway of side effects during the itinerary of anti-malignancy therapy.
To compare the effect of salt water rinse, sugar – free chewing gum and tooth brushing on the pH of saliva. 30 participants were included in the study. Baseline pH was measured using a digital pH meter. The participants were asked to swish their mouths with sucrose solution, and pH was measured. They were randomly divided into three groups namely; Group A –salt water rinse, Group B-sugar-free chewing gum and Group C- tooth brushing. After the intervention, the final pH was measured, and the recordings were compared. Repeated measures ANOVA test was used to compare the pH at Baseline, after rinsing and after an intervention. Bonferroni post-hoc test was done to do pair-wise comparison within the groups. There was significant difference pH at baseline and after intervention in all the three groups with Group B showing better results followed by Group C and then Group A. Sugar-free chewing gum significantly increased the pH of the saliva followed by tooth brushing and then salt water rinse.
Mobile phones have been important Electronic devices in our life. Consequently, Home automation and security system becomes one of the prominent futures on mobile devices. In this paper, we have developed the android application that interfaces with the security system using wifi direct technology. The wifi technology is relatively new as compared to other technologies and there is huge potential of its growth and practical application. The android application loaded on mobile devices, can connect with security system and easy to use GUI. The application developed to command lock, unlock or video monitoring of the home. The security system then acts on these command and responds to the user. The CMOS camera and the motion detector are attached with security system for remote surveillance.
People's health is becoming increasingly crucial to track in this 'New Normal' post-COVID-19 modern environment. The need of the hour is for modern technology that uses sensory sensors to track and record critical characteristics while also communicating with others. Keeping track of all of the medical factors is difficult. Collecting post-operative data of persons with non-communicable disorders such as diabetes and heart disease on a regular basis on an ongoing basis The technology we're talking about is intended for those who are confined to their homes. Especially when leaving the house and being exposed to the outside world is forbidden. This paper made a recommendation. For obtaining patient data, a breakthrough health management system based on the Internet of Things (IoT) has been developed. medical characteristics in both urban and rural settings. Keywords: Remote patient monitoring(RMP) , healthcare delivery, e-health arctitechure, telemedicine.
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