Antitumor activity of polysaccharide extracted from Pleurotus ostreatus mycelia against gastric cancer in vitro and in vivo

Abstract

The present study aimed to determine the antitumor effects of polysaccharides extracted from Pleurotus ostreatus mycelium on gastric cancer in vitro and in vivo. Polysaccharides were extracted from Pleurotus ostreatus mycelium and an antitumor component, known as Pleurotus ostreatus mycelium polysaccharides 2 (POMP2), with a relative molecular weight of 29 kDa, was then sequentially purified using Sephadex G200 size-exclusion chromatography and diethylaminoethyl-52 cellulose ion-exchange chromatography. The MTT method was used to determine the proliferation of BGC-823 cells treated with POMP2; cell migration assay, colony formation assay and in vivo antitumor tests were used to assess the effect of POMP2 on migration, cell survival and the in vivo tumor formation of BGH-823 cells. Results of the MTT assay indicated that POMP2 had a marked inhibitory effect on the BGC-823 human gastric cancer cell line; when administered at a concentration of 400 mg/l for 72 h, the rate of inhibition was 35.6%. In addition, the colony forming capacity of the BGC-823 cells was significantly reduced following treatment with POMP2. A migration assay indicated that the invasive capabilities of the BGC-823 cells were also significantly inhibited by POMP2. Furthermore, in vivo tests of mice engrafted with BGC-823 cancer cells demonstrated that both tumor weight and volume were markedly reduced following two weeks of treatment with POMP2. The results of the present study suggested that the polysaccharide POMP2 may have a potential application as a natural antitumor treatment for gastric cancer.

Exopolysaccharide-peptide complex from oyster mushroom (Pleurotus ostreatus) protects against hepatotoxicity in rats

“…Furthermore, PSP extract was cytotoxic in the human liver cancer cell line HepG2. These results are consistent with those of [ 32 ] in human colorectal and leukaemia cell lines, with [ 33 ] against gastric cancer in vitro and in vivo , and with [ 34 ] in HepG2, HCT 116 and HeLa cell lines. Additionally, previous reports demonstrated that extracts from P. ostreatus suppressed proliferation of breast cancer (MCF-7, MDA-MB-231) and colon cancer (HT-29, HCT-116) cells, without affecting proliferation of epithelial mammary MCF-10A and normal colon FHC cells [ 5 ].…”

Mushroom Polysaccharides: Chemistry and Antiobesity, Antidiabetes, Anticancer, and Antibiotic Properties in Cells, Rodents, and Humans

“…The vast literature (more than 1600 citations in the PubMed) on the isolation, structural elucidation, and bioactivities of mushroom polysaccharides includes reports on the following additional mushroom cultivars: Agaricus blazei [ 35 ], Agaricus brasiliensis [ 36 ], Amanita ponderosa [ 37 ], oyster mushroom [ 38 ], Auricularia polytricha [ 39 ], Boletus edulis [ 40 ], Cookeina tricholoma [ 41 ], Cordyceps militaris [ 42 ], Entoloma lividoalbum [ 43 ], Gleoestereum incarnatum [ 44 ], Ganoderma lucidum [ 45 ], Grifola frondosa [ 46 , 47 ], Hohenbuehelia serotina [ 48 ], Hypsizygus marmoreus [ 49 ], Iliodiction cibarium [ 50 , 51 ], Lactarius deliciosus [ 52 ], Lentinus edodes [ 31 , 53 ], Macrolepiota dolichaula [ 54 ], Phellinus igniarius [ 55 ], Phellinus linteus [ 56 ], Phellinus pini [ 57 ], Pholiota adiposa [ 58 ], Pholiota nameko [ 59 ], Pleurotus eryngii [ 60 ], Pleurotus ostreatus [ 55 , 61 ], Termitomyces heimii [ 62 ], Tricholoma matsutake [ 63 , 64 , 65 ], Tricholoma mongolicum [ 66 ]. A journal reviewer noted that it is, however, important to determine if the isolated polysaccharides contain polyphenolic or other bioactive compounds that might affect their activities.…”

Structural characterization and determination of biological activities for different polysaccharides extracted from tree mushroom species

“…Pleurotus is a large group of mushrooms with high nutritional value and therapeutic properties, as well as a wide range of biotechnological and environmental applications (Knop, Yarden, & Hadar, ). P. ostreatus polysaccharides possess antioxidant, anticancer, and antitumor properties (Cao, Liu, Yang, Hou, & Li, ; Uddin Pk et al, ). The differences in physicochemical and structural properties including molecular weights, monosaccharide compositions, glycosidic bonds, and chain conformation of the polysaccharides are known to affect the pharmacological activities of polysaccharides.…”