Antitumor and Immunomodulatory Effect ofGastrodia elataon Colon CancerIn VitroandIn Vivo

Abstract

Gastrodia elata Blume (GE) is a well-known kind of herb that has been used in traditional medicine for thousands of years. The extrusion of raw materials from it could improve flavor and enhance bioavailability in food and drug development. The purpose of this study is to investigate antitumor and immune boosting effects of extruded GE in human colon carcinoma cells, splenocytes, and mice-bearing CT26 colon carcinoma cell. Treatment with 100[Formula: see text][Formula: see text]g/mL of extruded GE decreased cell viability and induced the expression of Caspase-3 and Bax in HT29 cells ([Formula: see text]). When we performed DAPI staining, apoptotic bodies with condensed chromatin and fragmented nuclei, known as indicative of apoptotic morphology, increased 24[Formula: see text]h after treatment with 100[Formula: see text][Formula: see text]g/mL of extruded GE. Treatments with extruded GE significantly promoted splenocyte proliferation and IL-2 or IFN-[Formula: see text] secretion, compared with that of control cells ([Formula: see text]). The administration of extruded GE of 200 mg/kg/day decreased tumor growth and Ki-67 or [Formula: see text]-catenin expression in mice ([Formula: see text]). Additionally, we investigated the contents of compounds in extruded GE extracts using ultra performance liquid chromatography. The contents of p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde in extruded GE were 2.97[Formula: see text]mg/g and 0.04[Formula: see text]mg/g, respectively. It was supposed that antitumor and immunomodulatory effects of extruded GE might exert by the p-hydroxylbenzyl alcohol and p-hydroxybenzaldehyde of many compositions analyzed from extruded GE. These results suggest that extruded GE have the potential to be developed into a natural pharmaceutical and functional food as a cancer chemopreventive agent.

Vanillin and 4-hydroxybenzyl alcohol attenuate cognitive impairment and the reduction of cell proliferation and neuroblast differentiation in the dentate gyrus in a mouse model of scopolamine-induced amnesia

“…These compounds have been received increasing interest due to their potential biological properties such as anti-tumor, anti-oxidant, and anti-inflammatory actions [12131415]. Additionally, with their high attention, many researchers have demonstrated potential neuroprotective properties of vanillin and 4-HBA against neuronal injury using in vitro and in vivo experiments.…”

TCM monotherapy achieves significant efficacy in crizotinib-refractory advanced NSCLC with brain metastasis

“…Experiments proved that Chinese herbal medicine and its extracts can inhibit the growth of tumor cells or prevent the occurrence and development of tumors in various ways. TCM, such as Rhizoma Dioscorea, [26] Rhizoma Arisaematis, [27] Bombyx Batryticatus, [28] Coptis Chinensis, [29] Fructus Lycii, [30,31] Cyathula Officinalis, [32] Astragalus Membranaceus, [33][34][35] Phellodenron Amurense, [36] Cornus officinalis, [37] Gastrodiiae Elata, [38] Ziziphus Jujuba, [39] Polygala Tenuifolia, [40] and Eclipta Prostrata [41] have been confirmed the effect of antitumor. Berberine, the extract of Rhizoma Coptidis, can directly induce apoptosis.…”

The Improving Effect ofGastrodia elataBlume on DSS-induced Colitis in Mice

“…Gastrodia elata (GE) Blume is a belong to the to the Orchidaceae family and used for treating nervous diseases such as headaches, migraine, dizziness, and epilepsy (Matias et al, 2016;Zhan et al, 2016). A number of studies have shown that GE possess a variety of pharmacological activities including antiinflammatory, analgesic, anti-oxidative, memory-improving and anti-aging, anti-virus and anti-tumor activities (Zhan et al, 2016, Kim et al, 2017. However, there has been no precise information on whether GE regulates intestinal inflammation.…”