Antitumor effect of isoquercetin on tissue vasohibin expression and colon cancer vasculature

Silva, Daniel de Castilho da; Orfali, G.D.C.; Santana, Maycon Giovani; Palma, J.K.Y.; Assunção, I.R.D.O.; Marchesi, Isadora Moraes; Grizotto, A.Y.K.; Martínez, Natalia; Felliti, Simone; Pereira, José Aires; Priolli, Denise Gonçalves

doi:10.18632/oncotarget.28181

Cited by 7 publications

(3 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, when the tumor length was ≤ 5 cm, the majority of CDFI blood flow grades were grade 0, suggesting that the occurrence and development of colon cancer were significantly associated with the generation of tumor vessels [ 22 ]. In colon cancer, the association between blood vessels and clinicopathological factors has been controversia [ 23 ]. The studies of Chen et al and Li et al as well as our present study did not show any significant correlation [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

Correlation between color doppler flow pattern and molecular biology in elderly patients with colon cancer

et al. 2023

View full text Add to dashboard Cite

Objective To investigate the correlation between the grade and type of color Doppler flow imaging (CDFI) and tumor-related cytokines in elderly patients with colon cancer. Methods Seventy-six elderly patients with colorectal cancer admitted to Zhejiang Provincial People’s Hospital from July 2020 to June 2022 were selected. CDFI was used to analyze the blood flow grade and distribution type of tumor tissues, and ELISA was used to detect the levels of tumor-related cytokines in serum. Preoperative clinical data were collected and analyzed, and the correlation between measured cytokine levels and CDFI analysis results was further explored. Results CDFI blood flow grade showed significant difference in the different lengths, invasion depths and lymph node metastasis of tumors (all P < 0.001). In addition, serum levels of TNF-α, IL-6 and VEGF also showed statistical difference in all above different tumor-related factors (all P < 0.001). Further Pearson correlation analysis showed that CDFI blood flow grade and distribution types were both significantly positively correlated with above serum cytokine levels (r > 0, all P < 0.001). Kaplan-Meier survival analysis showed that both CDFI blood flow grade and distribution types were poor prognostic factors in elderly patients with colon cancer. Regression analysis showed that serum levels of TNF-α, IL-6 and VEGF were independent risk factors for poor prognosis of colon cancer in elderly patients. Conclusion CDFI blood flow grade and tumor tissue distribution have potential significant correlations with tumor-associated cytokines in the serum of colon cancer patients. CDFI blood flow grading technique provides an important imaging method for dynamic observation of angiogenesis and blood flow changes in elderly patients with colon cancer. Abnormal changes in serum levels of tumor-related factors can be used as sensitive indicators to evaluate the therapeutic effect and prognosis of colon cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Correlation between color doppler flow pattern and molecular biology in elderly patients with colon cancer

et al. 2023

View full text Add to dashboard Cite

show abstract

“…It is also called isoquercetin, isotrifoliin, or quercetin 3-glucoside. IQC has been reported to have efficacy against various diseases and has anti-cancer [ 11 ], anti-fatigue [ 12 ], anti-diabetes [ 13 , 14 , 15 ], and neuroprotective effects [ 16 , 17 , 18 ]. IQC is also known to have a broad spectrum of antiviral activities.…”

Section: Introductionmentioning

confidence: 99%

Antiviral Effect of Isoquercitrin against Influenza A Viral Infection via Modulating Hemagglutinin and Neuraminidase

Cho

Lee

Yeul

2022

IJMS

View full text Add to dashboard Cite

Isoquercitrin (IQC) is a component abundantly present in many plants and is known to have an anti-viral effect against various viruses. In this study, we demonstrate that IQC exhibits strong anti-influenza A virus infection, and its effect is closely related to the suppression of hemagglutinin (HA) and neuraminidase (NA) activities. We used green fluorescent protein-tagged Influenza A/PR/8/34 (H1N1), A/PR/8/34 (H1N1), and HBPV-VR-32 (H3N2) to evaluate the anti-IAV effect of IQC. The fluorescence microscopy and fluorescence-activated cell sorting analysis showed that IQC significantly decreases the levels of GFP expressed by IAV infection, dose-dependently. Consistent with that, IQC inhibited cytopathic effects by H1N1 or H3N2 IAV infection. Immunofluorescence analysis confirmed that IQC represses the IAV protein expression. Time-of-addition assay showed that IQC inhibits viral attachment and entry and exerts a strong virucidal effect during IAV infection. Hemagglutination assay confirmed that IQC affects IAV HA. Further, IQC potently reduced the NA activities of H1N1 and H3N2 IAV. Collectively, IQC prevents IAV infection at multi-stages via virucidal effects, inhibiting attachment, entry and viral release. Our results indicate that IQC could be developed as a potent antiviral drug to protect against influenza viral infection.

show abstract

“…This immunodeficient feature made the athymic mice relevant to the study of drug action ( Juul et al, 1992 ) and development ( Kelland, 2004 ), besides cytotoxicity research ( Budzynski and Radzikowski, 1994 ) and several diseases, including autoimmune ( Volpe et al, 1993 ) and cancer ( Szadvari et al, 2016 ). Regarding the latter, this model has been largely applied in distinct contexts associated with neoplasms, including breast ( Osborne et al, 1985 ), colorectal ( Giavazzi et al, 1986 ; da Silva et al, 2022 ), prostate ( van Weerden and Romijn, 2000 ), liver ( Yoysungnoen et al, 2008 ), lung ( Liu et al, 2012 ), pancreatic ( Zhao et al, 2013 ) and neurological cancers ( de Oliveira et al, 2019 ). Therefore, based on the exponential increase in global oncology cost over the last 10 years (2011 = US$ 56 billion; 2021 = US$ 187 billion) ( Statista, 2022 ) and the high cancer mortality rate ( Moss et al, 2020 ), the BALB/c (Nu/Nu) mice have become an important model onto new therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Validity of the peak velocity to detect physical training improvements in athymic mice

et al. 2022

Self Cite

View full text Add to dashboard Cite

This study comprises two complementary experiments with athymic Balb/c (Nu/Nu) mice. In experiment 1, the aim was to verify the reproducibility of the peak velocity (VPeak) determined from the incremental test. The second experiment aimed to assess the VPeak sensitivity to prescribe and detect modulations of the physical training in athymic nude mice. Sixteen mice were submitted to two incremental treadmill tests separated by 48-h (Experiment 1). The test consisted of an initial warm-up of 5 minutes. Subsequently, animals initiated the tests at 8 m min−1 with increments of 2 m min−1 every 3 minutes. The VPeak was determined as the highest velocity attained during the protocol. In experiment 2, these animals were randomly allocated to an exercise group (EG) or a control group (CG). The training protocol consisted of 30-min of treadmill running at 70% of the VPeak five times a week for 4 weeks. High indexes of reproducibility were obtained for VPeak (Test = 19.7 ± 3.6 m min−1; Retest = 19.2 ± 3.4 m min−1; p = 0.171; effect size = 0.142; r = 0.90). Animals from the EG had a significant increase of VPeak (Before = 18.4 ± 2.7 m min−1; After = 24.2 ± 6.0 m min−1; p = 0.023). Conversely, a significant decrease was observed for the CG (Before = 21.1 ± 3.9 m min−1; After = 15.9 ± 2.7 m min−1; p = 0.038). The VPeak is a valid parameter for exercise prescription in studies involving athymic nude mice.

show abstract

Antitumor effect of isoquercetin on tissue vasohibin expression and colon cancer vasculature

Cited by 7 publications

References 74 publications

Correlation between color doppler flow pattern and molecular biology in elderly patients with colon cancer

Correlation between color doppler flow pattern and molecular biology in elderly patients with colon cancer

Antiviral Effect of Isoquercitrin against Influenza A Viral Infection via Modulating Hemagglutinin and Neuraminidase

Validity of the peak velocity to detect physical training improvements in athymic mice

Contact Info

Product

Resources

About