Antitumor effect of the seashell protein Haishengsu on Ehrlich ascites tumor: an experimental study

Liu, Jizhu; Chen, Shou-Guo; Zhang, Bin; Wang, Chunbo; Li, Guangyao; Wang, Lexin

doi:10.1007/s11418-009-0346-4

Cited by 7 publications

(7 citation statements)

References 3 publications

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“…In this study, we determined %ILS after CCS treatment and showed dose-dependent improvements in survival time (C1: 19.23%; C2: 23.08% and C3: 34.61%). The same was reported in a study of the protein Haishengsu from Tegillarca granosa L. This study showed an increase in %ILS in animals inoculated with EAC cells, indicating inhibition of tumor growth (Liu et al, 2009). …”

Section: Discussionsupporting

confidence: 77%

Anticancer activity of flavonol and flavan-3-ol rich extracts fromCroton celtidifoliuslatex

Biscaro

Parisotto

Zanette

et al. 2013

Pharmaceutical Biology

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Context: Croton celtidifolius Baill (Euphorbiaceae) is a tree found in the Atlantic Forest in Southern Brazil, where it is commonly known as ''Sangue-de-Dragão''. Its red latex is used traditionally for treating ulcers, diabetes and cancer. Objective: To evaluate antitumor activities of Croton celtififolius latex in vitro and in vivo. Material and methods: Phytochemical analyses were conducted using HPLC-DAD-MS. Cytotoxic, nuclease and pro-apoptotic properties were determined using the tetrazolium salt assay (MTT), plasmid DNA damage assay and ethidium bromide (EB)/acridine orange methods, respectively, and antitumor activity was determined in the Ehrlich ascites carcinoma (EAC) mouse model. Results: Phytochemical studies indicated a high phenol content of flavonols (45.67 AE 0.24 and 18.01 AE 0.23 mg/mL of myricetin and quercetin, respectively) and flavan-3-ols (114.12 AE 1.84 and 1527.41 AE 16.42 mg/L of epicatechin and epigallocatechin, respectively) in latex. These compounds reduced MCF-7 and EAC cell viability in the MTT assay (IC 50 ¼ 169.0 AE 1.8 and 187.0 AE 2.2 mg/mL, respectively). Latex compounds caused significant DNA fragmentation and increased the number of apoptotic cells (negative control (NC), 12%; latex, 41%) as indicated by differential staining in the EB/acridine orange assay. The in vivo latex treatment at 3.12 mg/kg/day reduced the body weight by 7.57 AE 2.04 g and increased median survival time to 17.5 days when compared to the NC group (13.0 days). In addition, the highest latex concentration inhibited tumor growth by 56%. Discussion and conclusion: These results agree with ethno-pharmacological reports showing cytotoxicity and antitumor activity of C. celtidifolius latex. The mechanism of antitumor action may be related to direct DNA fragmentation that reduces survival and induces apoptosis.

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Section: Discussionsupporting

confidence: 77%

Anticancer activity of flavonol and flavan-3-ol rich extracts fromCroton celtidifoliuslatex

Biscaro

Parisotto

Zanette

et al. 2013

Pharmaceutical Biology

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“…The mechanisms by which HSS improves the chemosensitivities of acute leukemia are not entirely clear. Previous in vitro works have shown that HSS reduces the expression of multi-drug resistance associated genes, MDR-1, BCR-ABL, and sorcin [2,[4][5][6][7][8]10,11]. P-gp is a membrane transport protein located in the cell membrane encoded by MDR-1 genes.…”

Section: Discussionmentioning

confidence: 99%

“…The natural products may have an adjuvant effect when added to conventional chemotherapy in patients with cancers [1,2]. Haishengsu (HSS) is a purified protein from Tegillarca granosa L. (Order Arcoida) which is a sea creature that contains a high concentration of proteins and vitamin B 12 [3][4][5][6][7][8][9][10][11]. When used as an adjuvant therapy, HSS was reported to improve clinical outcomes of patients undergoing conventional chemotherapy for lung or renal cancer [2,8].…”

Section: Introductionmentioning

confidence: 99%

Marine drug Haishengsu increases chemosensitivity to conventional chemotherapy and improves quality of life in patients with acute leukemia

Zhang

Liu

et al. 2016

Biomedicine & Pharmacotherapy

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“…The extracts of T. L. granosa are components of Chinese medicine formula used to treat many cancers and inflammatory conditions for some centuries. [1][2][3] Recently, the biological activities of several proteins purified from T. L. granosa have been evaluated in vitro and in vivo. 4,5 We isolated a novel seashell protein with a molecular weight of approximately 15 kDa (15-23 kDa) from T. L. granosa and named it Haishengsu (HSS).…”

Section: Introductionmentioning

confidence: 99%

“…L. granosa is a type of Malaysian shellfish. For centuries, the extracts of T. L. granosa have been included in Chinese medicinal formulas that have been used to treat many cancers and inflammatory diseases [1][2][3]. Recently, several proteins have been purified from T. L. granosa and their biological activities have been evaluated 19,20.…”

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confidence: 99%

Haishengsu, a Protein from Shellfish Tegillarca L. granosa, Inhibits the Growth and the Activity of Matrix Metalloproteinases-2 and -9 in Human Lung Carcinoma

et al. 2011

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Haishengsu (HSS) is a seashell protein extracted from Tegillarca L. granosa, a type of Malaysian shellfish. Previous in vitro studies showed that HSS might possess biological anticancer activity. In this combined in vitro and in vivo study, we investigated the inhibitory effects of HSS on tumor growth, invasion, and metastasis using human lung carcinoma cell lines A549 and NCI-H292, both intensely positive for matrix metalloproteinases-2 (MMP-2) and MMP-9. HSS significantly inhibited the proliferation of A549 and NCI-H292 as estimated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The transwell chamber assay showed that HSS effectively blocked the invasion and migration of the carcinoma cells through the reconstituted extracellular matrix (Matrigel). Gelatin zymography analysis revealed that the secretion and activity of MMP-2 and MMP-9 in the supernatants of the cultured cells A549 and NCI-H292 were decreased after treatment with HSS. The levels of MMP-2 and MMP-9 in these cancer cells were further examined by Western blot assay in which a significant decrease of MMP-2 and MMP-9 was observed in A549 and NCI-H292 cells after 24 h of exposure to HSS. The anticancer activity of HSS was verified in a mouse model in which HSS delayed the growth of A549 xenografts after 3 weeks of oral administration. Inhibition of MMP-2 and MMP-9 expression was also demonstrated in the A549 xenografts as determined by Western blot analysis. These results suggest that HSS is a novel seashell protein that cannot only inhibit tumor growth but also prevent tumor invasion and metastasis through suppressing the activity of MMP-2 and MMP-9.

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Antitumor effect of the seashell protein Haishengsu on Ehrlich ascites tumor: an experimental study

Cited by 7 publications

References 3 publications

Anticancer activity of flavonol and flavan-3-ol rich extracts fromCroton celtidifoliuslatex

Anticancer activity of flavonol and flavan-3-ol rich extracts fromCroton celtidifoliuslatex

Marine drug Haishengsu increases chemosensitivity to conventional chemotherapy and improves quality of life in patients with acute leukemia

Haishengsu, a Protein from Shellfish Tegillarca L. granosa, Inhibits the Growth and the Activity of Matrix Metalloproteinases-2 and -9 in Human Lung Carcinoma

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