Antitumor efficacy of Urtica sp. leaves extract: in vitro and in vivo studies

Abstract

Background: Natural products demonstrated potential for use in medicine and pharmaceuticals. Objective: This study evaluated the phytochemical composition of Urtica sp. leaves extract (USLE), and it's in vitro and in vivo antitumor effects. Methods: HepG-2 and MCF-7 cell lines were used to evaluate the IC50, apoptosis, and cell cycle analysis of USLE. Forty female CD-1 mice were equally divided as follows; Gp1 was control; Gp2, Gp3, and Gp4 were inoculated with 1 × 10 6 of Ehrlich ascitic carcinoma (EAC)-cells/mouse. Then, Gp3 had injected with Cisplatin (2 mg/kg) intraperitoneally (i.p). Gp4 had injected with USLE (100 mg/kg) i.p. as in Gp3. Total tumor volume, total tumor cell count, and live and dead EAC-cells were determined, also biochemical and histopathological investigations were evaluated. Results: Data showed that the USLE had cytotoxic and antitumor effects against the HepG-2, MCF-7, and EAC-bearing mice. Biochemical and histopathological investigations showed an improvement in the liver and kidney tissues upon treatment of EAC-bearing mice with USLE. Conclusion: The results showed that the USLE can stop tumor growth and cause tumor cells to die.

Urtica pilulifera leaves extract mitigates cadmium induced hepatotoxicity via modulation of antioxidants, inflammatory markers and Nrf-2 signaling in mice

“…After being shade-dried, the leaves were pulverized into powder. A mixture of 50 g of leaves powder and 500 mL of 70% ethanol was filtered, then the U. pilulifera leaves extract (UPLE) was dried ( Fakher El Deen et al, 2022 ).…”

Therapeutic, chemo-sensitizing, protective effects of natural products against cancerous cells: Preclinical and clinical studies

“…A new generation of anticancer medications is presently being employed in clinical trials because of advanced technology and substantial study on marine natural ingredients (Khalifa et al, 2019). Through strong growth inhibition of human tumor cells in vitro, in vivo, and in cancer clinical trials, a sizable number of metabolites originating from marine sources function as anticancer agents (Fakher El Deen et al, 2022). Most marine plant species are algae, and they have a wide range of chemical compositions.…”

Urtica pilulifera leaves exacerbate the cisplatin effect in Ehrlich ascites carcinoma-bearing mice

“…Seventy male Swiss albino mice (20 ± 2 g) were given drinking tap water and normal experimental pelleted animal food ad libitium. Mice were divided into seven groups as follows: Gp1 was used as a negative control, from Gp2 to Gp7 were inoculated with 1 × 10 6 EACcells/mouse, then Gp2 left as a positive control, Gp3 was injected with Cis (2 mg/kg), Gp4 was injected with UPLE (100 mg/kg) [25], Gp5 was co-treated with Cis (2 mg/kg) and UPLE (100 mg/kg). Gp6 was injected with the low dose of Cis (0.5 mg/kg), and Gp7 was co-treated with the low dose of Cis (0.5 mg/kg) and UPLE (100 mg/kg).…”