Antitumoral and Antimicrobial Activity of Surfactin Extracted from Bacillus subtilis KLP2015

Meena, Khem Raj; Sharma, Abhishek; Kanwar, Shamsher S.

doi:10.1007/s10989-019-09848-w

Cited by 52 publications

(27 citation statements)

References 44 publications

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“…It exhibits powerful therapeutic activities i.e. antimicrobial, antimycoplasma, antiviral, anti-inflammatory, antibacterial and antitumor 10,70,[72][73][74][75] . Additionally, it is widely applicable environmentally in biopesticides, food processing, pharmaceuticals, cosmetics and oil recovery [76][77][78] .…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G2/M arrest in hepatocellular carcinoma (HepG2) cell line

El-Garawani

El-Sabbagh

Abbas

et al. 2020

Sci Rep

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Marine bacterial strains are of great interest for their ability to produce secondary metabolites with anticancer potentials. Isolation, identification, characterization and anticancer activities of isolated bacteria from El-Hamra Lake, Wadi El-Natrun (Egypt) were the objectives of this study. The isolated bacteria were identified as a moderately halophilic alkaliphilic strain. Ethyl acetate extraction was performed and identified by liquid chromatography-mass spectrophotometry (LC-MS-MS) and nuclear magnetic resonance analysis (NMR). Cytotoxicity of the extract was assessed on the HepG2 cell line and normal human peripheral lymphocytes (HPBL) in vitro. Halomonas sp. HA1 extract analyses revealed anticancer potential. Many compounds have been identified including cyclo-(Leu-Leu), cyclo-(Pro-Phe), C17-sphinganine, hexanedioic acid, bis (2-ethylhexyl) ester, surfactin C14 and C15. The extract exhibited an IC 50 of 68 ± 1.8 μg/mL and caused marked morphological changes in treated HepG2 cells. For mechanistic anticancer evaluation, 20 and 40 µg/mL of bacterial extract were examined. The up-regulation of apoptosis-related genes' expression, P53, CASP-3, and BAX/ BCL-2 at mRNA and protein levels proved the involvement of P53-dependant mitochondrial apoptotic pathway. The anti-proliferative properties were confirmed by significant G 2 /M cell cycle arrest and PCNA down-regulation in the treated cells. Low cytotoxicity was observed in HPBL compared to HepG2 cells. In conclusion, results suggest that the apoptotic and anti-proliferative effects of Halomonas sp. HA1 extract on HepG2 cells can provide it as a candidate for future pharmaceutical industries. Abbreviations NMR Nuclear magnetic resonance LC-MS-MS Liquid chromatography-mass spectrophotometry MeOH Methanol DMSO Dimethyl sulphoxide

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G2/M arrest in hepatocellular carcinoma (HepG2) cell line

El-Garawani

El-Sabbagh

Abbas

et al. 2020

Sci Rep

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“…Several studies have focused on the microbial production of lipopeptides, by either wild type or genetically engineered strains of Bacillus ( Coutte et al, 2017 , 2013 ). It has been reported that lipopeptides produced by Bacillus strains exhibit various biological activities, such as anti-fungal, anti-inflammatory, anti-tumoral, anti-viral, and anti-platelet that makes them ideal candidates for their application as therapeutic agents and drug delivery systems ( Meena and Kanwar, 2015 ; Kourmentza et al, 2017 ; Meena et al, 2019 ). Therefore, they could potentially outcompete their synthetic counterparts in such high-value applications as they have the advantages of being biologically produced in a sustainable way, using renewable resources, while they expected to be less toxic towards humans and the environment ( Kopsahelis et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Lipopeptide Biosurfactants Against Foodborne Pathogen and Food Spoilage Microorganisms and Their Cytotoxicity

et al. 2021

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Lipopeptide biosurfactants produced by Bacillus sp. were assessed regarding their antimicrobial activity against foodborne pathogenic and food spoilage microorganisms. Both Gram-positive and Gram-negative bacteria were found not to be susceptible to these lipopeptides. However, mycosubtilin and mycosubtilin/surfactin mixtures were very active against the filamentous fungi Paecilomyces variotti and Byssochlamys fulva, with minimum inhibitory concentrations (MICs) of 1–16 mg/L. They were also active against Candida krusei, MIC = 16–64 mg/L. Moreover it was found that the antifungal activity of these lipopeptides was not affected by differences in isoform composition and/or purity. Furthermore their cytotoxicity tested on two different cell lines mimicking ingestion and detoxification was comparable to those of approved food preservatives such as nisin. Overall, for the first time here mycosubtilin and mycosubtilin/surfactin mixtures were found to have high antifungal activity against food relevant fungi at concentrations lower than their toxicity level hence, suggesting their application for extending the shelf-life of products susceptible to these moulds. In addition combining nisin with mycosubtilin or mycosubtiliin/surfactin mixtures proved to be an effective approach to produce antimicrobials with broader spectrum of action.

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“…The DEAE cellulose packed glass column (height 10 cm; diameter 1.5 cm) was equilibrated with 20 mM sodium phosphate buffer (pH 7.5) after activation by 0.5 M NaOH. Five mL of dialyzed biosurfactant preparation (4.0 mg protein) was loaded on the matrix in the column [ 23 ]. Column was equilibrated with 20 mM sodium phosphate buffer (pH 7.5).…”

Section: Methodsmentioning

confidence: 99%

“…Sephadex G-25 packed matrix was washed off with several column volume of 20 mM sodium phosphate buffer (pH 7.5).Pooled active fraction of the DEAE was loaded on the bed surface of Sephadex G-25 column and eluted with the sodium phosphate buffer (20 mM; pH 7.5) and fractions were collected [ 23 ]. Absorbance at 280 nm and emulsification activity was evaluated against the engine oil.…”

Section: Methodsmentioning

confidence: 99%

Purification and identification of a surfactin biosurfactant and engine oil degradation by Bacillus velezensis KLP2016

et al. 2021

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Engine oil used in automobiles is a threat to soil and water due to the recalcitrant properties of its hydrocarbons. It pollutes surrounding environment which affects both flora and fauna. Microbes can degrade hydrocarbons containing engine oil and utilize it as a substrate for their growth. Our results demonstrated that cell-free broth of Bacillus velezensis KLP2016 (Gram + ve, endospore forming; Accession number KY214239) recorded an emulsification index (E24%) from 52.3% to 65.7% against different organic solvents, such as benzene, pentane, cyclohexane, xylene, n-hexane, toluene and engine oil. The surface tension of the cell-free broth of B. velezensis grown in Luria–Bertani broth at 35 °C decreased from 55 to 40 mN m−1at critical micelle concentration 17.2 µg/mL. The active biosurfactant molecule of cell-free broth of Bacillus velezensis KLP2016 was purified by Dietheylaminoethyl-cellulose and size exclusion chromatography, followed by HPLC (RT = 1.130), UV–vis spectrophotometry (210 nm) and thin layer chromatography (Rf = 0.90). The molecular weight of purified biosurfactant was found to be ~ 1.0 kDa, based on Electron Spray Ionization-MS. A concentration of 1980 × 10–2 parts per million of CO2 was trapped in a KOH solution after 15 days of incubation in Luria–Bertani broth containing 1% engine oil. Our results suggest that bacterium Bacillus velezensis KLP2016 may promise a new dimension to solving the engine oil pollution problem in near future.

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Antitumoral and Antimicrobial Activity of Surfactin Extracted from Bacillus subtilis KLP2015

Cited by 52 publications

References 44 publications

RETRACTED ARTICLE: A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G2/M arrest in hepatocellular carcinoma (HepG2) cell line

RETRACTED ARTICLE: A newly isolated strain of Halomonas sp. (HA1) exerts anticancer potential via induction of apoptosis and G2/M arrest in hepatocellular carcinoma (HepG2) cell line

Antimicrobial Activity of Lipopeptide Biosurfactants Against Foodborne Pathogen and Food Spoilage Microorganisms and Their Cytotoxicity

Purification and identification of a surfactin biosurfactant and engine oil degradation by Bacillus velezensis KLP2016

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