Antitumoral Effect of Laurinterol on 3D Culture of Breast Cancer Explants

Abstract

Macroalgae represent an important source of bioactive compounds with a wide range of biotechnological applications. Overall, the discovery of effective cytotoxic compounds with pharmaceutical potential is a significant challenge, mostly because they are scarce in nature or their total synthesis is not efficient, while the bioprospecting models currently used do not predict clinical responses. Given this context, we used three-dimensional (3D) cultures of human breast cancer explants to evaluate the antitumoral effect of laurinterol, the major compound of an ethanolic extract of Laurencia johnstonii. To this end, we evaluated the metabolic and histopathological effects of the crude extract of L. johnstonii and laurinterol on Vero and MCF-7 cells, in addition to breast cancer explants. We observed a dose-dependent inhibition of the metabolic activity, as well as morphologic and nuclear changes characteristic of apoptosis. On the other hand, a reduced metabolic viability and marked necrosis areas were observed in breast cancer explants incubated with the crude extract, while explants treated with laurinterol exhibited a heterogeneous response which was associated with the individual response of each human tumor sample. This study supports the cytotoxic and antitumoral effects of laurinterol in in vitro cell cultures and in ex vivo organotypic cultures of human breast cancer explants.

Laurinterol from Laurencia johnstonii eliminates Naegleria fowleri triggering PCD by inhibition of ATPases

“…Cultures of human breast cancer explants treated with laurinterol showed a heterogeneous response, probably related with the individual response of each tumor sample. The study supports the cytotoxic and antitumoral effects of 1 both in in vitro cell cultures as in ex vivo organotypic cultures 25 .…”

“…Furthermore, the mitochondrial damage was also checked by measuring the generated ATP levels in 24 h. The observed results showed that laurinterol IC 90 treated amoebae presented a 97.36% decrease of ATP levels when compared to untreated cells. Moreover, since laurinterol has been reported as an inhibitor of the Na + /K + -ATPase sodium–potassium ion pump 25 , activity assays of known inhibitors including ouabain and furosemide were carried out. Amoebae showed to be ouabain-insensitive even at high concentrations of this product whereas furosemide showed an IC 50 of 0.71 ± 0.04 mM.…”

“…The marine environment is an interesting source of bioactive compounds which could be potential novel therapeutic agents against cancer and parasites, among others, as it has been previously described 25 . Furthermore, algae are currently the major source of these type of molecules being Laurencia genus one of the richest organisms among the reported ones so far 21 , 25 .…”

“…Furthermore, algae are currently the major source of these type of molecules being Laurencia genus one of the richest organisms among the reported ones so far 21,25 . In a recent study by García-Davis 25 , the study of species adapted to live www.nature.com/scientificreports/ therapeutic options. Furthermore, the current options are not fully effective and lack of availability worldwide creates the need to find alternative options and sources 4,13 .…”

Antimycobacterial Activity of Laurinterol and Aplysin from Laurencia johnstonii

“…Among marine organisms, algae of the genus Laurencia are considered one of the richest sources of novel compounds with a wide range of bioactive properties, such as the brominated sesquiterpenes laurinterol (1) and aplysin (2) ( Figure 1) [10]. Laurinterol has been evaluated for its antibacterial [11][12][13], cytotoxic [13][14][15], antifouling [16], anti-Acanthamoeba [17], Na/K ATPase inhibition [18], insecticidal, and repellent properties [19]. Meanwhile, aplysin has shown moderate cytotoxic activity and has been evaluated for its mechanisms of action in different biological models [20][21][22][23].…”