Antitumour activity of <i>Ganoderma lucidum</i>, an edible mushroom, on intraperitoneally implanted lewis lung carcinoma in synergenic mice

Furusawa, Eiichi; Chou, S.C.; Furusawa, Shinobu; Hirazumi, Anne; Dang, Yanan

doi:10.1002/ptr.2650060604

Cited by 75 publications

(46 citation statements)

References 10 publications

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“…3) Previous studies of G. lucidum have mainly concentrated on the medicinal properties of this mushroom. Limited information is available on the activities of the enzymes which modify oligo-and polysaccharides present in the mushroom, though preliminary reports [4][5][6][7] have shown that G. lucidum used galactose as the best carbon source for producing potentialized ganoderan having anti-tumor and anti-complementary activity. We found that cultivated G. lucidum produced a wide variety of glycosidases, and had strong a-galactosidase activity.…”

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confidence: 99%

Purification and Characterization of Thermostable α-Galactosidase fromGanoderma lucidum

Sripuan

Aoki

Yamamoto

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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confidence: 99%

Purification and Characterization of Thermostable α-Galactosidase fromGanoderma lucidum

Sripuan

Aoki

Yamamoto

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…Its medical effects on cancer, hypertension, hepatitis, and hypercholesterolemia have been demonstrated by pharmacological studies in the past two decades. [1][2][3][4] This fungus has attracted considerable attention partly because the polysaccharides isolated from the fruit bodies and the mycelium have shown antitumor and hyperglycemic activity. 3,5,6) However, the active components in the spores of G. lucidum have rarely been studied owing to the difficulty in collection and sporoderm-breaking of the spores.…”

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confidence: 99%

Purification, Characterization, and Modification of T Lymphocyte-Stimulating Polysaccharide from Spores of <i>Ganoderma lucidum</i>

et al. 2002

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Ganoderma lucidum, a well-known Chinese medicinal fungus, has been used to promote health and longevity in East Asian countries. Its medical effects on cancer, hypertension, hepatitis, and hypercholesterolemia have been demonstrated by pharmacological studies in the past two decades. [1][2][3][4] This fungus has attracted considerable attention partly because the polysaccharides isolated from the fruit bodies and the mycelium have shown antitumor and hyperglycemic activity. 3,5,6) However, the active components in the spores of G. lucidum have rarely been studied owing to the difficulty in collection and sporoderm-breaking of the spores. Recently, with the successful cultivation of G. lucidum indoors on a large scale and a breakthrough in sporodermbreaking technology, much attention has been paid to chemical components of G. lucidum spores 7,8) and their versatile biological activities.9,10) More recently, we have reported an immunomodulating polysaccharide from the spores of G. lucidum. 11) In the present study, we deal with the purification and characterization of the T lymphocyte-stimulating polysaccharide, based on the activity-guided principle, from the hot-water extract of the sporoderm-broken spores of G. lucidum. A series of sulfated or carboxymethylated derivatives of the polysaccharide were successively prepared and their structures were elucidated by chemical and spectral methods. The solution conformation and T lymphocyte proliferation effect of the polysaccharide and its derivatives are compared and discussed. ExperimentalMaterials Spores of G. lucidum were collected in Shanxi Province, PR China. It was identified by Dr. Xiu-Lan Huang and stored as a voucher specimen (no. 99064) in the Herbarium of Phytochemistry Department of Shanghai Institute of Materia Medica, Shanghai, PR China. Before use, the sporoderm of the spores was ultrasonographically broken and the broken ratio was estimated about 60-80% by electronic microscopy. T-Dextran in a series of different molecular weights was obtained from Pharmacia. Concanavalin (Con A) was from Sigma and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was from Fluka. Medium RPMI-1640 was purchased from Gibco Laboratories. All other reagents were of the highest quality available and were used without further purification.Isolation and Purification of the T Lymphocyte-Stimulating Polysaccharide The sporoderm-broken spores of G. lucidum (1.0 kg), previously defatted with 95% alcohol, were decocted for 6 h with 10 l of water to half the original volume, and the residue was again decocted with 8 l of water. The combined aqueous extract was deproteinated with trichloroacetic acid, and the resulting aqueous fraction was extensively dialyzed against running water for 3 d and then against distilled water for 1 d (molecular weight cutoff 3000-5000 Da). The retentate was concentrated under reduced pressure to a small volume (1.8 l), and 4 volumes of ethanol were added stepwise with stirring at 4°C. Then the mixture was stored overnight at Ϫ10°C. T...

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“…Similar to the results obtained using the mouse sarcoma 180 cell model, a polysaccharide extract of Ganoderma lucidum has also been shown to inhibit the growth of Lewis lung carcinoma cells in mice (19,21). The sulfate derivatives of D-glucans demonstrate a significantly higher antitumor activity against Ehrlich ascites carcinoma (22).…”

Section: Discussionmentioning

confidence: 63%

Inhibitory effect of a water-soluble extract from the culture medium of Ganoderma lucidum (Rei-shi) mycelia on the development of pulmonary adenocarcinoma induced by N-nitrosobis (2-hydroxypropyl) amine in Wistar rats

Kashimoto

Hayama²,

Kamiya³

et al. 2006

Oncol Rep

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Abstract.A water-soluble extract from the culture medium of Ganoderma lucidum (Rei-shi) mycelia (MAK) has been shown to exert a potent chemopreventive effect. The present study was designed to investigate the effects of dietary MAK supplementation on the development of lung tumors initiated by N-nitrosobis (2-hydroxypropyl) amine (BHP) in male Slc:Wistar rats. A total of 77 animals, 6 weeks of age, were divided into 5 groups and given BHP (2,000 ppm) in their drinking water for 10 weeks. The normal controls were not supplied with BHP. After treatment with the carcinogen, the rats were fed a normal control MF solid diet, or the same diet containing MAK (1.25%, 2.5% or 5%) for 12 weeks. Macroscopically, all the doses of MAK reduced the number of nodules, and the effect of 5% MAK was found to be especially significant. Microscopically, an increase in the number of proliferating cell nuclear antigen (PCNA)-negative tumors and a decrease in the number of tumors strongly positive for PCNA were observed in the tissue sections from the rats that had received all the doses of MAK. The present results thus indicate that dietary supplementation with MAK inhibits the development of lung tumors, suggesting that MAK may be a potent chemopreventive agent against lung carcinogenesis.

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Antitumour activity of Ganoderma lucidum, an edible mushroom, on intraperitoneally implanted lewis lung carcinoma in synergenic mice

Cited by 75 publications

References 10 publications

Purification and Characterization of Thermostable α-Galactosidase fromGanoderma lucidum

Purification and Characterization of Thermostable α-Galactosidase fromGanoderma lucidum

Purification, Characterization, and Modification of T Lymphocyte-Stimulating Polysaccharide from Spores of <i>Ganoderma lucidum</i>

Inhibitory effect of a water-soluble extract from the culture medium of Ganoderma lucidum (Rei-shi) mycelia on the development of pulmonary adenocarcinoma induced by N-nitrosobis (2-hydroxypropyl) amine in Wistar rats

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