Inhibitory effect of a water-soluble extract from the culture medium of Ganoderma lucidum (Rei-shi) mycelia on the development of pulmonary adenocarcinoma induced by N-nitrosobis (2-hydroxypropyl) amine in Wistar rats

Abstract

Abstract.A water-soluble extract from the culture medium of Ganoderma lucidum (Rei-shi) mycelia (MAK) has been shown to exert a potent chemopreventive effect. The present study was designed to investigate the effects of dietary MAK supplementation on the development of lung tumors initiated by N-nitrosobis (2-hydroxypropyl) amine (BHP) in male Slc:Wistar rats. A total of 77 animals, 6 weeks of age, were divided into 5 groups and given BHP (2,000 ppm) in their drinking water for 10 weeks. The normal controls were not supplied with BHP. After treatment with the carcinogen, the rats were fed a normal control MF solid diet, or the same diet containing MAK (1.25%, 2.5% or 5%) for 12 weeks. Macroscopically, all the doses of MAK reduced the number of nodules, and the effect of 5% MAK was found to be especially significant. Microscopically, an increase in the number of proliferating cell nuclear antigen (PCNA)-negative tumors and a decrease in the number of tumors strongly positive for PCNA were observed in the tissue sections from the rats that had received all the doses of MAK. The present results thus indicate that dietary supplementation with MAK inhibits the development of lung tumors, suggesting that MAK may be a potent chemopreventive agent against lung carcinogenesis.

Anti-Cancerous Potential of Polysaccharide Fractions Extracted from Peony Seed Dreg on Various Human Cancer Cell Lines Via Cell Cycle Arrest and Apoptosis

“…In general, high molecular weight contributes to anti-cancerous activity of plant polysaccharides, whereas reports also suggested that even low MW equally influence the anti-cancerous activities ( Kashimoto et al, 2006 ). One study revealed that partially cellulose digested Aloe polysaccharide fractions within the size of 5–400 kDa had the most potent anti-cancerous activity, depicting the importance of size of polysaccharide for its biological activity ( Im et al, 2005 ).…”

Active lipids of Ganoderma lucidum spores-induced apoptosis in human leukemia THP-1 cells via MAPK and PI3K pathways

“…The fruit bodies, cultured mycelia and spores of Ganoderma lucidum were reported to be effective in the treatment of chronic hepatopathy, hypertension, hyperglycemia and cancer (Hong et al, 2004;Sliva, 2006;Liu et al, 2009). Many reports showed Ganoderma lucidum to possess antiproliferative effects on many cancers, such as acute myelogenous leukemia, lung cancer, breast cancer, and prostate cancer (Chui et al, 2006;Kashimoto et al, 2006;Thyagarajan et al, 2006;Xie et al, 2006;Yue et al, 2006). It was also demonstrated that Ganoderma lucidum extract has a profound activity against leukemia, lymphoma and multiple myeloma cells and may be a novel adjunctive therapy for the treatment of hematologic malignancies (Müller et al, 2006).…”

Ganoderma lucidum polysaccharides in human monocytic leukemia cells: from gene expression to network construction

“…Ganoderma lucidum ( G. lucidum , Reishi or Ling-Zhi) has been used in traditional Chinese medicine as an anti-tumor medication or as an immuno-modulator. Many reports showed Reishi extracts to possess anti-proliferative effects on many cancers, such as acute myelogenous leukemia [ 1 ], lung cancer [ 2 ], breast cancer [ 3 ], colorectal cancer [ 4 ], bladder cancer [ 5 ] and prostate cancer [ 6 , 7 ]. A fucose-containing polysaccharide fraction (F3), isolated from the water-soluble Reishi extract, is able to stimulate spleen cell proliferation and cytokine expression [ 8 - 11 ].…”