Antiulcerogenic Effect of Brazilian Propolis Formulation in Mice

Abstract

Propolis is a natural resin used around the world to treatment of several diseases. We evaluated the effect of Brazilian propolis (P1) in guinea pig isolated trachea in vitro contracted with histamine, in paw oedema induced by histamine in mice and anti-ulcerogenic effect of propolis formulation in mice. We used a P1 extract from South of Brazil to produce a formulation containing sodium bicarbonate and propolis dry extract (2%). In the guinea pig trachea Brazilian propolis extract (10 µg/mL) produced a non-significant inhibition in the cumulative response curve for histamine. However, the dose of 100 µg/mL shown had a significant inhibitory effect on the contraction induced by histamine (51% ± 5%). In the mouse paw oedema induced by intraplantar injection of histamine (50 μg/paw), the results showed that the treatment of the mice with P1 (10 or 100 mg/Kg (p.o) or 1 or 10 mg/Kg (i.p)), 0.5 h beforehand, significantly inhibited the paw oedema with maximal inhibition (MI) of 25% or 42% by p.o. route or 22% or 37% by i.p. route, respectively. Finally we have pre-treated mice with P1 formulation (20 mg/Kg or 40 mg/Kg, p.o, one time) or vehicle (powder) in animals submitted to diclofenac-induced ulcer, after 8 h P1 formulation inhibited of 43% or 83% of ulcers scores, respectively. Results showed that P1 formulation inhibited the ulcerogenic effect induced by diclofenac. Results suggested also, at least in part, the antiulcerogenic effect of formulation containing standardized extract of propolis can be due to the antagonistic action on the histaminergic system.

Anti-inflammatory activity of Tetragronula species from Indonesia

“…Recent research conducted by Paulino et al (2015) compared the anti-inflammatory effects of sodium diclofenac and propolis. Both compounds demonstrated anti-inflammatory effects; however, sodium diclofenac can cause ulcerogenic disease.…”

“…Both compounds demonstrated anti-inflammatory effects; however, sodium diclofenac can cause ulcerogenic disease. However, additional research has indicated that propolis may inhibit the formation of ulcers caused by sodium diclofenac (Paulino et al, 2015).…”

Molecular and cellular mechanisms of the effects of Propolis in inflammation, oxidative stress and glycemic control in chronic diseases

“…Another experimental study indicated the antihistaminergic, antiinflammatory, anti-H. pylori activities and antiacid, of Propolis that could be used for the treatment of gastric ulceration [57]. Artepillin C and another phenolic mixtures available in Brazilian Propolis clears free radicals and decrease the oxidative stress relevant to inflammation [57]. Treatment with Propolis attenuated the levels of LPS (lipopolysaccharide) and down-regulated the TLR4 pathway and expressions of inflammatory markers in muscle of experimental mice, and improved serum triacylglycerols and glucose levels, also decreased inflammatory cytokines and endotoxemia by inhibiting dysbiosis in mice experimented with a high-fat diet [58].…”

“…This study demonstrated transformation of the pear-shaped cell and decrease beating frequency of flagellar in the trophozoites [ 56 ]. Another experimental study indicated the antihistaminergic, antiinflammatory, anti- H. pylori activities and antiacid, of Propolis that could be used for the treatment of gastric ulceration [ 57 ]. Artepillin C and another phenolic mixtures available in Brazilian Propolis clears free radicals and decrease the oxidative stress relevant to inflammation [ 57 ].…”

Efficacy of olibanum and propolis medicinal extracts versus metronidazole in Giardia lamblia experimentally infected mice

“…Moreover, antimicrobial besides antiulcer and immunomodulatory properties could be detected [14]. Propolis was promisingly proposed to manage giardiasis with minimal side effects [15].…”