Antiurolithiatic Activity of Extract and Oleanolic Acid Isolated from the Roots of Lantana camara on Zinc Disc Implantation Induced Urolithiasis

Abstract

The present study was done to evaluate the antiurolithiatic activity of ethanolic extract of roots (ELC 200 mg/kg) and oleanolic acid (OA 60 mg/kg, O.A. 80 mg/kg, O.A. 100 mg/kg) isolated from roots of Lantana camara in albino wistar male rats using zinc disc implantation induced urolithiatic model. The group in which only zinc disc was implanted without any treatment showed increase in calcium output (23 ± 2.7 mg/dL). Cystone receiving animals showed significant protection from such change (P < 0.01). Treatment with OA and ELC significantly reduced the calcium output at a dose of OA 60 mg/kg (P < 0.01), OA 80 mg/kg (P < 0.01), ELC 200 mg/kg (P < 0.01), and OA 100 mg/kg (P < 0.001), as compared with zinc disc implanted group. The average weight of zinc discs along with the deposited crystals in the only disc implanted group was found to be 111 ± 8.6 mg. Group that received Cystone 500 mg/kg showed significant reduction in the depositions (P < 0.001). Similarly, the rats which received OA and ELC showed reduced formation of depositions around the zinc disc (P < 0.001). The X-ray images of rats also showed significant effect of OA and ELC on urolitiasis. Thus, OA and ELC showed promising antiurolithiatic activity in dose dependant manner.

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“…[19] Thus, this study involves the induction of hyperoxaluria in male albino rats with the oral administration of EG which stimulates the nidus formation, calcium oxalate saturation, crystal aggregation, and retention within kidney tubules. [20] Male rats were selected for the study due to resemblance of urinary system with the human and also earlier studies have shown that frequency of stone deposition was significantly high in male as compare to female rats. [21] This study demonstrates that ethanolic root extract and petroleum ether fraction showed good antiurolithic activity in a dosedependent manner as compared the standard drug cystone.…”

Antiurolithiatic activity of Abelmoschus moschatus seed extracts against zinc disc implantation-induced urolithiasis in rats

“…[26] This suggests obstruction of urinary bladder due to the formation of urinary calculi. In this study, the CAM and MAM treatment increased urine output, and this may be due to prevention of stone formation or their direct diuretic effect which reduced supersaturation of urine with precipitating substances and thereby stone formation.…”

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“…Then the rats were sacrificed and zinc disc implants/stones were removed from the bladder and dried. Stones taken out were weighed [6][7][8][9][10] .…”