Antiviral activities of 2,6-diaminopurine-based acyclic nucleoside phosphonates against herpesviruses: In vitro study results with pseudorabies virus (PrV, SuHV-1)

Zouharová, Darina; Lipenská, Ivana; Fojtíková, Martina; Kulich, Pavel; Neča, Jiřı́; Slaný, Michal; Kovařčík, K.; Turanek-Knotigova, Pavlina; Hubatka, František; Čelechovská, Hana; Mašek, Josef; Koudelka, Štěpán; Procházka, Lubomír; Eyer, Luděk; Plocková, Jana; Bartheldyová, Eliška; Miller, Andrew D.; Růžek, Daniel; Raška, Milan; Janeba, Zlatko; Tuřánek, Jaroslav

doi:10.1016/j.vetmic.2016.01.010

Cited by 13 publications

(6 citation statements)

References 37 publications

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“…The cytotoxicity of curcumin on Vero E6 cells was evaluated using an MTT assay [73]. Briefly, Vero E6 cells were seeded in 96-well plates at a density of 1.0 × 10 4 cells/well in DMEM supplemented with 2% FBS.…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

et al. 2021

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Due to the scarcity of therapeutic approaches for COVID-19, we investigated the antiviral and anti-inflammatory properties of curcumin against SARS-CoV-2 using in vitro models. The cytotoxicity of curcumin was evaluated using MTT assay in Vero E6 cells. The antiviral activity of this compound against SARS-CoV-2 was evaluated using four treatment strategies (i. pre–post infection treatment, ii. co-treatment, iii. pre-infection, and iv. post-infection). The D614G strain and Delta variant of SARS-CoV-2 were used, and the viral titer was quantified by plaque assay. The anti-inflammatory effect was evaluated in peripheral blood mononuclear cells (PBMCs) using qPCR and ELISA. By pre–post infection treatment, Curcumin (10 µg/mL) exhibited antiviral effect of 99% and 99.8% against DG614 strain and Delta variant, respectively. Curcumin also inhibited D614G strain by pre-infection and post-infection treatment. In addition, curcumin showed a virucidal effect against D614G strain and Delta variant. Finally, the pro-inflammatory cytokines (IL-1β, IL-6, and IL-8) released by PBMCs triggered by SARS-CoV-2 were decreased after treatment with curcumin. Our results suggest that curcumin affects the SARS-CoV-2 replicative cycle and exhibits virucidal effect with a variant/strain independent antiviral effect and immune-modulatory properties. This is the first study that showed a combined (antiviral/anti-inflammatory) effect of curcumin during SARS-CoV-2 infection. However, additional studies are required to define its use as a treatment for the COVID-19.

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Section: Cytotoxicity Assaymentioning

confidence: 99%

Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

et al. 2021

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“…In the present study, we identify the active compounds responsible for the readthrough activity of H7 extract, one of which is 2,6-diaminopurine (DAP). DAP has been used previously for antileukemia treatment and is reported to exert antiviral [36][37][38][39] and miRNA inhibition activity 40 . It is demonstrated here to be an efficient and exclusive corrector of UGA nonsense mutations in both immortalized human cells and an in vivo mouse model.…”

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confidence: 99%

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations

et al. 2020

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Nonsense mutations cause about 10% of genetic disease cases, and no treatments are available. Nonsense mutations can be corrected by molecules with nonsense mutation readthrough activity. An extract of the mushroom Lepista inversa has recently shown highefficiency correction of UGA and UAA nonsense mutations. One active constituent of this extract is 2,6-diaminopurine (DAP). In Calu-6 cancer cells, in which TP53 gene has a UGA nonsense mutation, DAP treatment increases p53 level. It also decreases the growth of tumors arising from Calu-6 cells injected into immunodeficient nude mice. DAP acts by interfering with the activity of a tRNA-specific 2′-O-methyltransferase (FTSJ1) responsible for cytosine 34 modification in tRNA Trp. Low-toxicity and high-efficiency UGA nonsense mutation correction make DAP a good candidate for the development of treatments for genetic diseases caused by nonsense mutations.

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“…When cells had grown to approximately 95 % confluence, they were washed three times with PBS and incubated with different concentrations of curcumin (10,20,30,40,50,60,70,80, 90 and 100 µM) for 36 h. DMSO-treated cells served as controls (0 µM). After washing with PBS, the cells were analysed by an MTT assay as described previously [13]. Cell survival rate was determined as drug average OD value/control average OD value.…”

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confidence: 99%

Antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro

Wang

Liu

et al. 2020

Journal of General Virology

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Emerging coronaviruses represent serious threats to human and animal health worldwide, and no approved therapeutics are currently available. Here, we used Transmissible gastroenteritis virus (TGEV) as the alpha-coronavirus model, and investigated the antiviral properties of curcumin against TGEV. Our results demonstrated that curcumin strongly inhibited TGEV proliferation and viral protein expression in a dose-dependent manner. We also observed that curcumin exhibited direct virucidal abilities in a dose-, temperature- and time-dependent manner. Furthermore, time-of-addition assays showed that curcumin mainly acted in the early phase of TGEV replication. Notably, in an adsorption assay, curcumin at 40 µM resulted in a reduction in viral titres of 3.55 log TCID50 ml–1, indicating that curcumin possesses excellent inhibitory effects on the adsorption of TGEV. Collectively, we demonstrate for the first time that curcumin has virucidal activity and virtual inhibition against TGEV, suggesting that curcumin might be a candidate drug for effective control of TGEV infection.

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Antiviral activities of 2,6-diaminopurine-based acyclic nucleoside phosphonates against herpesviruses: In vitro study results with pseudorabies virus (PrV, SuHV-1)

Cited by 13 publications

References 37 publications

Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

Curcumin Inhibits In Vitro SARS-CoV-2 Infection In Vero E6 Cells through Multiple Antiviral Mechanisms

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations

Antiviral and virucidal effects of curcumin on transmissible gastroenteritis virus in vitro

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