Antiviral activities of plant-derived indole and β-carboline alkaloids against human and avian influenza viruses

Hegazy, Akram; Mahmoud, Sara H.; Elshaier, Yaseen A.M.M.; Shama, Noura M. Abo; Nasr, Nasr Fawzy; Ali, Mohamed A.; El-Shazly, Assem M.; Mostafa, Islam; Mostafa, Ahmed

doi:10.1038/s41598-023-27954-0

Cited by 22 publications

(4 citation statements)

References 62 publications

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“…Small molecule compound screening has been widely used to identify inhibitors and medicines to be used in potential prevention and treatment strategies for various viral infections, such as severe acute respiratory syndrome coronavirus 2 [14,15], influenza A virus [16], dengue virus [17], and hepatitis C virus [18] infections.…”

Section: Sc Significantly Inhibits Prrsv Replicationmentioning

confidence: 99%

The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-κB signalling pathway

Liu,

Zhu,

Wang

et al. 2023

Vet Res

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Porcine reproductive and respiratory syndrome (PRRS) is a serious infectious disease and one of the major causes of death in the global pig industry. PRRS virus (PRRSV) strains have complex and diverse genetic characteristics and cross-protection between strains is low, which complicates vaccine selection; thus, the current vaccination strategy has been greatly compromised. Therefore, it is necessary to identify effective natural compounds for the clinical treatment of PRRS. A small molecule library composed of 720 natural compounds was screened in vitro, and we found that Sanggenon C (SC) was amongst the most effective natural compound inhibitors of PRRSV infection. Compared with ribavirin, SC more significantly inhibited PRRSV infection at both the gene and protein levels and reduced the viral titres and levels of protein expression and inflammatory cytokine secretion to more effectively protect cells from PRRSV infection and damage. Mechanistically, SC inhibits activation of the NF-κB signalling pathway by promoting TRAF2 expression, thereby reducing PRRSV replication. In conclusion, by screening natural compounds, we found that SC suppresses PRRSV infection by regulating the TRAF2/NF-κB signalling pathway. This study contributes to a deeper understanding of the therapeutic targets and pathogenesis of PRRSV infection. More importantly, our results demonstrate that SC has potential as a candidate for the treatment of PRRS.

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Section: Sc Significantly Inhibits Prrsv Replicationmentioning

confidence: 99%

The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-κB signalling pathway

Liu,

Zhu,

Wang

et al. 2023

Vet Res

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“…Normal bioactive compounds give assurance and diminish the hazard of different constant infections such as cancer, diabetes, and obesity 6,7 . The novel coronavirus serious, intense respiratory disorder coronavirus 2 (SARS-CoV-2), a recently developing viral respiratory infection, is known to cause COVID-19, which is appallingly spreading around the globe and there's no stoppage.…”

Section: Introductionmentioning

confidence: 99%

Astaxanthin-Based Drug Design: A Virtual Screening Study for COVID-19 Main Protease Inhibition

Safaei-Ghomi,

Lordejani,

Marimuthu

et al. 2024

Preprint

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The main purpose of this study was to assess the capability of Astaxanthin, a carotene compound, as a potential organic inhibitor of coronavirus main protease by computational techniques and approaches. To design new inhibitors based on Astaxanthin structure, compare them with the co-crystal inhibitor N3, as a potent inhibitor of the COVID-19 main protease, and determine if the new structures have similar or better binding affinity to the COVID-19 main protease, different virtual screening methods such as Molecular Docking, Structure-Based Drug Design, Ligand-Based Drug Design, Molecular Dynamic Simulation, and Synthesis Accessibility Prediction were used. The hit compounds were selected based on their highest binding energy ranging from − 8.3 to -9.2, Lipinski's (Pfizer's) rule of five, and the Synthesis Accessibility scores to indicate the easy to synthesis compounds. The stability of ligands with the proteins at their active sites was further confirmed through molecular dynamics. Our results suggested that the selected compounds had favorable interactions at the binding pocket of the coronavirus main protease, and exhibited better binding affinities and simpler structures compared to N3. The suggested compounds could be used as lead compounds for further drug development and optimization. However, more experimental and clinical validation is required to approve their efficacy and safety.

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“…Some studies have also demonstrated the efficacy of βCs in vivo [9,16]. Their spectrum of antiviral activity includes DNA and RNA viruses, such as HSV [15][16][17], HIV [18], poliovirus (PV) [19], tobacco mosaic virus (TMV) [20], dengue virus 2 (DENV-2) [21] and avian and human influenza A viruses [22]. Particularly, HSV-1 was the first virus reported to be affected by certain βC alkaloids (Eudistomins) obtained from the colonial tunicate Eudistoma olivaceum [23].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

Gonzalez,

Vizoso-Pinto,

Erra-Balsells

et al. 2024

IJMS

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Herpes simplex virus (HSV) infections are highly widespread among humans, producing symptoms ranging from ulcerative lesions to severe diseases such as blindness and life-threatening encephalitis. At present, there are no vaccines available, and some existing antiviral treatments can be ineffective or lead to adverse effects. As a result, there is a need for new anti-HSV drugs. In this report, the in vitro anti-HSV effect of 9,9′-norharmane dimer (nHo-dimer), which belongs to the β-carboline (βC) alkaloid family, was evaluated. The dimer exhibited no virucidal properties and did not impede either the attachment or penetration steps of viral particles. The antiviral effect was only exerted under the constant presence of the dimer in the incubation media, and the mechanism of action was found to involve later events of virus infection. Analysis of fluorescence lifetime imaging data showed that the nHo-dimer internalized well into the cells when present in the extracellular incubation medium, with a preferential accumulation into perinuclear organelles including mitochondria. After washing the host cells with fresh medium free of nHo-dimer, the signal decreased, suggesting the partial release of the compound from the cells. This agrees with the observation that the antiviral effect is solely manifested when the alkaloid is consistently present in the incubation media.

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Antiviral activities of plant-derived indole and β-carboline alkaloids against human and avian influenza viruses

Cited by 22 publications

References 62 publications

The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-κB signalling pathway

The natural compound Sanggenon C inhibits PRRSV infection by regulating the TRAF2/NF-κB signalling pathway

Astaxanthin-Based Drug Design: A Virtual Screening Study for COVID-19 Main Protease Inhibition

In Vitro Effect of 9,9′-Norharmane Dimer against Herpes Simplex Viruses

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