2018
DOI: 10.1096/fj.201701361r
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Antiviral activity of double‐stranded RNA‐binding protein PACT against influenza A virus mediated via suppression of viral RNA polymerase

Abstract: PACT is a double-stranded RNA-binding protein that has been implicated in host-influenza A virus (IAV) interaction. PACT facilitates the action of RIG-I in the activation of the type I IFN response, which is suppressed by the viral nonstructural protein NS1. PACT is also known to interact with the IAV RNA polymerase subunit PA. Exactly how PACT exerts its antiviral activity during IAV infection remains to be elucidated. In the current study, we demonstrated the interplay between PACT and IAV polymerase. Induct… Show more

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Cited by 16 publications
(23 citation statements)
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“…qRT-PCR was carried out essentially as previously described (46,47). In brief, total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany).…”
Section: Qrt-pcrmentioning
confidence: 99%
See 1 more Smart Citation
“…qRT-PCR was carried out essentially as previously described (46,47). In brief, total RNA was extracted with the RNeasy Mini Kit (Qiagen, Hilden, Germany).…”
Section: Qrt-pcrmentioning
confidence: 99%
“…The RNA interference (RNAi) experiment was performed as previously described (45,47). Short interfering RNAs (siRNAs) were transfected into THP-1 cells with Lipofectamine 2000 48 h before infection with SARS-CoV.…”
Section: Rna Interferencementioning
confidence: 99%
“…HEK293T cells were maintained in DMEM (Life Technologies) with 10% FBS (Life Technologies) as described. 31 Plasmid transfection to HEK293T cells was performed with Gene-Juice (Novagen, Gibbstown, NJ, USA) according to manufacturer's protocol. Plasmid transfection to THP-1 cells was performed with GeneXPlus (ATCC) according to manufacturer's protocol.…”
Section: Cell Culture Transfection and Recombinant Iavmentioning
confidence: 99%
“…32 Recombinant IAVs WSN-PF and WSN-PΔF were rescued and titrated as previously described. 31 and Western blotting using the appropriate antibodies as described. 29 For co-immunoprecipitation assay, cells were lysed in NP40 lysis buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.4, 2 mM EDTA, 1% NP-40 and 0.5% sodium deoxycholate) and the target proteins were immunoprecipitated as described.…”
Section: Cell Culture Transfection and Recombinant Iavmentioning
confidence: 99%
“…Compromising PACT in IAV-infected A549 cells led to the enhancement of vRNA transcription and replication and IFNβ production. In addition, vRNA replication was increased by knocking down PACT in both A549 cells and IFN-insufficient Vero cells 28 .…”
mentioning
confidence: 99%