Antiviral activity of glycoprotein GP-1 isolated from Streptomyces kanasensis ZX01

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BACKGROUND Plant systemic resistance induced by botanical compounds is a promising alternative method of disease management. The natural product berberine, usually used as an antimicrobial in medicine, has been proven to have antifungal activity in agriculture. To investigate the induced resistance imparted by berberine, the effect of berberine against tobacco mosaic virus (TMV) and the mechanism governing this effect were determined. RESULT Berberine exhibited considerable in vivo anti‐TMV activity of up to 68.3% but had no in vitro direct effect on TMV. Moreover, berberine could induce immune responses against TMV in tobacco, including the hypersensitive reaction (HR), accumulation of H2O2, increases in defense enzymes and overexpression of pathogenesis‐related (PR) proteins. In addition, upregulation of salicylic acid (SA) biosynthesis genes PAL, CM1, ICS, PBS3 and the enzyme benzoic acid 2‐hydroxylase (BA2H) confirmed that SA was involved in the defensive signals. Berberine can induce crop resistance against TMV, Phytophthora nicotianae, Botrytis cinerea and Blumeria graminis in the greenhouse. CONCLUSION This paper highlights the use of berberine in manipulating tobacco to generate defense responses against TMV, which can be attributed to SA‐mediated induced resistance. The paper provides a theoretical basis for the application of berberine as a resistance activator and for further research on induced resistance by botanical natural product. © 2019 Society of Chemical Industry

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Berberine induces resistance against tobacco mosaic virus in tobacco

Guo

Yan

Ren

et al. 2020

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Anti‐TMV activity and mode of action of three alkaloids isolated from Chelidonium majus

Guo

Liu

et al. 2020

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BACKGROUND: Plant viral diseases are difficult to control and have caused serious damage to the agricultural industry. Recently, botanical biopesticides characterized by environment friendly, safe to non-target organism and not as susceptible to produce drug resistance, have exhibited great potential to be developed as antiviral agents. To screen the natural products with antiviral effect, three alkaloids possessed anti-tobacco mosaic virus (TMV) activity were isolated from Chelidonium majus and the modes of action were investigated. RESULT: The anti-TMV effect of crude extracts at 10 mg mL −1 was 51.73%. Bioassay-guided fractionation and isolation of the compounds with anti-TMV activity were performed on the methanol extract of C. majus yielding three bioactive alkaloids namely: chelerythrine (1), chelidonine (2), and sanguinarine (3). The results of bioassay showed that chelerythrine exhibited great inactivation, proliferation inhibition and protection effects against TMV at 0.5 mg mL −1 with the efficiency of 72.67%, 77.52% and 59.34%, respectively. Chelidonine at 0.1 mg mL −1 can provide 54.90% and 64.45% inhibitions on TMV through inducing resistance in two kinds of tobacco. Sanguinarine showed a weaker protection for resisting TMV in comparison to chelerythrine and chelidonine. CONCLUSION: Chelerythrine and chelidonine displayed significant inhibitions on TMV with different modes of action. These results provided important evidence that the extracts in C. majus might be a potential source of new drugs in controlling virus disease agriculturally.

Identification of anti‐TMV active flavonoid glycosides and their mode of action on virus particles from Clematis lasiandra Maxim

et al. 2021

BACKGROUND Tobacco mosaic virus (TMV) is a disreputable plant pathogen that causes a decline in the quality and yield of various economic crops. Natural products are important potential sources of biopesticides to control TMV. This study focuses on the discovery of anti‐TMV active flavonoid glycosides and their mode of action on TMV particles from Clematis lasiandra Maxim. RESULTS A new benzoyl acylated flavonoid glycoside, kaempferol 3‐O‐(2′′‐benzoyl)‐β‐d‐glucopyranosyl‐7‐O‐α‐l‐rhamnopyranoside (1), and nine known flavonoids (2–10) were identified first from C. lasiandra. The hydroxyl group at C‐7, E‐p‐coumarate at C‐6′′ in the Glc of C‐6, and the glucuronic acid at C‐3 were functional groups for the antiviral flavonoid glycosides. Flavonoids 2, 5, and 6 showed higher inactivation efficacies of 64.62% to 82.54% compared with ningnanmycin at 500 μg ml−1. The protective and curative efficacies for 2 and 5 were 57.44–59.00% and 41.17–43.92% at 500 μg ml−1, respectively. Compound 5 showed higher TMV systemic resistance with control efficacies of 41.64%, 36.56% and 27.62% at concentrations of 500, 250 and 125 μg ml−1 compared with ningnanmycin in K326 tobaccos, respectively. Compound 5 can directly fracture TMV particles into small fragments combining with the fusion phenomena, and TMV‐CP was an important target for 5 to break TMV particles. CONCLUSION Flavonoid glycosides from C. lasiandra showed potent antiviral activities against TMV with multiple modes of action including inactivation, protective and curative effects, and inducing systemic resistance. TMV‐CP was an important target for active flavonoid glycosides to fracture TMV particles. The results provided evidence that flavonoid glycosides from C. lasiandra have the potential to control TMV.