Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

Song, Jae-Hyoung; Yeo, Sang-Gu; Hong, Eock Kee; Lee, Bora; Kim, Jin-Won; Kim, JeongHoon; Jeong, HyeonGun; Kwon, Yong-Soo; Kim, HyunPyo; Lee, Sang‐Won; Park, Jae‐Hak; Ko, Hyun‐Jeong

doi:10.4062/biomolther.2013.108

Cited by 56 publications

(42 citation statements)

References 26 publications

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“…Compounds extracted from Chinese herbs have showed promising antiviral effect against EV71 infection (Chiang et al, 2005;Lin et al, 2009;Song et al, 2014;Zhang et al, 2014). The application of these compounds resulted in reduced viral replication, inhibited apoptosis of the infected cells, or decreased mortality of the infected mice.…”

Section: Introductionmentioning

confidence: 98%

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Lin

Qin

et al. 2015

Virus Research

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Section: Introductionmentioning

confidence: 98%

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Lin

Qin

et al. 2015

Virus Research

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“…Coxsackievirus B3 (CVB3), one of the most well-studied enteroviruses, causes viral meningitis, myocarditis and pancreatitis [1,2]. In addition, enterovirus 71 (EV71) is a causative agent of hand-foot-mouth disease and also of severe neurological symptoms, which can lead to even death [3][4][5]. However, despite the increasing public threat, no effective therapy is currently available for the treatment of these infections.…”

Section: Introductionmentioning

confidence: 99%

Gemcitabine, a broad-spectrum antiviral drug, suppresses enterovirus infections through innate immunity induced by the inhibition of pyrimidine biosynthesis and nucleotide depletion

et al. 2017

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Gemcitabine, an anti-cancer chemotherapy drug, has additionally shown the antiviral activity against a broad range of viruses and we also have previously reported its synergistic antiviral activity with ribavirin against enteroviruses. As a cytidine analog, gemcitabine has been reported to have an inhibitory activity on the pyrimidine biosynthesis. In addition, a few inhibitors of the pyrimidine biosynthesis have shown to induce the innate immunity in a yet-to-be-determined manner and inhibit the virus infection. Thus, we also investigated whether the anti-enteroviral activity of gemcitabine is mediated by innate immunity, induction of which is related with the inhibition of the pyrimidine synthesis. In this study, we found that the addition of exogenous cytidine, uridine and uridine mono-phosphate (UMP) effectively reversed the antiviral activity of gemcitabine in enterovirus-infected as well as enteroviral replicon-harboring cells, demonstrating gemcitabine’s targeting of the salvage pathway. Moreover, the expression of several interferon (IFN)-stimulated genes (ISGs) was significantly induced by the treatment of gemcitabine, which was also suppressed by the co-treatment with cytidine. These results suggest that the antiviral activity of gemcitabine involves ISGs induced by the inhibition of the pyrimidine biosynthesis.

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“…Assays of antiviral activity and cytotoxicity were evaluated by the sulforhodamine (SRB) method using the cytopathic effect (CPE) induced by viral infection as recently reported (Song et al ., 2014). The infectivity of each virus was determined by measuring intact cells attached to plates after addition of CCID 50 (50% cell culture infective dose) of CVB3 virus.…”

Section: Methodsmentioning

confidence: 99%

Antiviral Activity of Chrysin Derivatives against Coxsackievirus B3 in vitro and in vivo

Song

Kwon

Jang

et al. 2015

Biomolecules & Therapeutics

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Chrysin is a 5,7-dihydroxyflavone and was recently shown to potently inhibit enterovirus 71 (EV71) by suppressing viral 3C protease (3Cpro) activity. In the current study, we investigated whether chrysin also shows antiviral activity against coxsackievirus B3 (CVB3), which belongs to the same genus (Enterovirus) as EV71, and assessed its ability to prevent the resulting acute pancreatitis and myocarditis. We found that chrysin showed antiviral activity against CVB3 at 10 μM, but exhibited mild cellular cytotoxicity at 50 μM, prompting us to synthesize derivatives of chrysin to increase the antiviral activity and reduce its cytotoxicity. Among four 4-substituted benzyl derivatives derived from C(5) benzyl-protected derivatives 7, 9–11 had significant antiviral activity and showed the most potent activity against CVB3 with low cytotoxicity in Vero cells. Intraperitoneal injection of CVB3 in BALB/c mice with 1×106 TCID50 (50% tissue culture infective dose) of CVB3 induced acute pancreatitis with ablation of acinar cells and increased serum CXCL1 levels, whereas the daily administration of 9 for 5 days significantly alleviated the pancreatic inflammation and reduced the elevation in serum CXCL1 levels. Collectively, we assessed the anti-CVB3 activities of chrysin and its derivatives, and found that among 4-substituted benzyl derivatives, 9 exhibited the highest activity against CVB3 in vivo, and protected mice from CVB3-induced pancreatic damage, simultaneously lowering serum CXCL1 levels.

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Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

Cited by 56 publications

References 26 publications

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Pyrrolidine dithiocarbamate inhibits enterovirus 71 replication by down-regulating ubiquitin–proteasome system

Gemcitabine, a broad-spectrum antiviral drug, suppresses enterovirus infections through innate immunity induced by the inhibition of pyrimidine biosynthesis and nucleotide depletion

Antiviral Activity of Chrysin Derivatives against Coxsackievirus B3 in vitro and in vivo

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