Antiviral Activity of N-Phenyl-N′-arylthiourea Derivatives against some Rhinoviruses

Abstract

The effect of 12 derivatives of N-phenyl-N′-aryl- or alkylthiourea, inhibitors of human enteroviruses and foot-and-mouth disease virus, on reproduction of some rhinoviruses (H-17, B-632) in HeLa Bristol cells was studied. As screening methods both the multicycle growth test in roller tube cultures and two variants of plaque inhibition tests were employed. The compounds selected were tested in one-step growth cycle set-up. We established that N-phenyl-N′-4-hydroxyphenyl-thiourea (V-24) and N-phenyl-N′-2-carboxyphenylthiourea (V-17) have a distinct inhibitory effect on the growth of rhinovirus H-17, and N-phenyl-N′-2-hydroxy-5-nitrophenylthiourea (V-25) inhibited strongly the multiplication of rhinovirus B-632.

Antirhinovirus activity of purine nucleoside analogs

“…The search for effective antirhinovirus agents has yielded a wealth of compounds belonging to widely varying chemical classes: thiosemicarbazones (20), isoquinolines (35,43), triazinoindoles (27), guanidines (5), benzoates (30), furanyls (2), benzimidazoles (12), thiourea (18), diketones (14,16,17), flavans (3,32), flavones (25), chalcones (24), nitrobenzenes (34,41), and isoxazoles (15,31). The most recently developed antirhinovirus compounds (3,15,24,31) reach their MICs within the range of 0.001 to 0.01 ,ug/ml.…”

Picornavirus inhibitors

“…36) are inhibitors of coxsackie virus, poliovirus, FMDV and rhinoviruses. Drug concentrations of approximately 2/~g/mL of N-phenyl-N'-3hydroxyphenyl-thiourea (PTU-23) reduced rhinovirus growth by one-log (Galabov et al, 1977). The potency and antiviral index of N-phenyl-N'-arylthiourea are rather low and probably these compounds block an early step of rhinovirus replication after adsorption (Galabov et al, 1977;Galabov, 1979).…”

“…Drug concentrations of approximately 2/~g/mL of N-phenyl-N'-3hydroxyphenyl-thiourea (PTU-23) reduced rhinovirus growth by one-log (Galabov et al, 1977). The potency and antiviral index of N-phenyl-N'-arylthiourea are rather low and probably these compounds block an early step of rhinovirus replication after adsorption (Galabov et al, 1977;Galabov, 1979). PTU-23 had activity against coxsackie infection in mice, whereas another derivative (PTU-24) was active against FMDV in mice and guinea pigs (Galabov, 1979).…”