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A study was made of antiviral activities in vitro of 54 derivatives of N-phenyl-N′-aryl or alkylthiourea with a basic structural formula C₆H₅NHCSNHR against poliovirus 1 (Mahoney). Eleven active compounds were selected by the Screening gradient-plaque-inhibiting test, some of which (N-phenyl-N′-4-carboxy-5-hydroxyphenylthiourea, N-phenyl-N′-3-hydroxyphenylthiourea) in a one-step growth cycle inhibit 99.4–99.9% of the virus yield. It was noted that, along with the -HN-CS-NH- group, the presence and spatial location of the hydroxyl group in the aryl or alkyl radical at N′-atom is of importance to antiviral activity.

The effect of 12 derivatives of N-phenyl-N′-aryl- or alkylthiourea, inhibitors of human enteroviruses and foot-and-mouth disease virus, on reproduction of some rhinoviruses (H-17, B-632) in HeLa Bristol cells was studied. As screening methods both the multicycle growth test in roller tube cultures and two variants of plaque inhibition tests were employed. The compounds selected were tested in one-step growth cycle set-up. We established that N-phenyl-N′-4-hydroxyphenyl-thiourea (V-24) and N-phenyl-N′-2-carboxyphenylthiourea (V-17) have a distinct inhibitory effect on the growth of rhinovirus H-17, and N-phenyl-N′-2-hydroxy-5-nitrophenylthiourea (V-25) inhibited strongly the multiplication of rhinovirus B-632.

SummaryThe effect of eleven derivatives of N-phenyl-N'-aryl-or alkylthiourea on the multiplication of Coxsackie B 1, ECHO 19 and foot-and-mouth disease viruses in cell cultures has been tested. In one-step growth cycle experiments N-phenyl-N'-4-earboxy-5-hydroxyphenylthiourea, N-phenyl-N'-3-hydroxyphenylthiourea and y-phenylthioureidobutyric acid inhibited 99.9% of the Coxsaekie ]31 and ECHO 19 virus yields. N-phenyl-N'-4-hydroxyphenylthiourea suppressed 99.65% of the foot-and-mouth disease virus yield.