Antiviral activity of trimeric 2',5'-oligoadenylic acid and some of its analogues

Tkachuk, Z. Yu.; Semernikova, L. I.; Tkachuk, V. V.; Reshotko, L. M.; Mikhailopulo, Igor A.; Matsuka, G. Kh.

doi:10.7124/bc.0003fc

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…2'-5'-A 3 epoxy exhibited the antiviral effect regardless of the mode of its delivery into the cell, while 2'-5'-A 3 xylo did not exhibit any antiviral effect on the above-mentioned viruses. The obtained results, therefore, permit to suggest that the studied olygoadenylates realize their antiviral properties on different groups of viruses [25].…”

Section: '-5'-a 3 Epoxy 2'-5'-a 3 Cord 2'-5'-a 3 Amino 2'-5'-a 3 Inosupporting

confidence: 51%

2'-5'-Oligoadenylates as a «tool» of innate immunity

Tkachuk

2013

Biopolym. Cell

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It is a matter of common knowledge, that «core» 2'-5'-oligoadenylates and their analogues posses broader biological activity than it can be predicted within the traditional «interferon» hypothesis. They exhibit immunomodulatory effects and are effective immunosuppressive agents upon organ and tissue transplantation. Oligoadenylates render antiviral activity against a wide range of viruses both DNA and RNA origin. These drugs regulate apoptosis, cell proliferation and possess antitumor activity. Such polyvalent activity is based on their ability to bind signaling proteins, slightly altering their conformation and modulating their activity. They can also change the secondary structure of both viral DNA and RNA, making them accessible for enzymatic cleavage. The hypothesis forwarded within this review is aimed at making an attempt to explain the mechanism of 2'-5'-oligoadenylates antiviral action. Our recent papers, containing experimental data to support this hypothesis, are discussed in this review

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Section: '-5'-a 3 Epoxy 2'-5'-a 3 Cord 2'-5'-a 3 Amino 2'-5'-a 3 Inosupporting

confidence: 51%

2'-5'-Oligoadenylates as a «tool» of innate immunity

Tkachuk

2013

Biopolym. Cell

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“…Attempts which have been most successful include immunotherapy with various combinations of interferon (14,19,60) or with neutralizing antibodies or antibody fragments (41,62). Limited data indicate mixed anticoronaviral activity for nucleoside analogs including riba- virin (11,16,18,35,44,59,71,78,82) and an assortment of other small molecules (8,10,13,55,69,80). Corticosteroid treatment has proved generally ineffective against coronaviruses (3,4).…”

Section: Discussionmentioning

confidence: 99%

Antisense Morpholino-Oligomers Directed against the 5′ End of the Genome Inhibit Coronavirus Proliferation and Growth†

Neuman

Stein

Kroeker

et al. 2004

J Virol

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Conjugation of a peptide related to the human immunodeficiency virus type 1 Tat represents a novel method for delivery of antisense morpholino-oligomers. Conjugated and unconjugated oligomers were tested to determine sequence-specific antiviral efficacy against a member of the Coronaviridae, Mouse hepatitis virus (MHV). Specific antisense activity designed to block translation of the viral replicase polyprotein was first confirmed by reduction of luciferase expression from a target sequence-containing reporter construct in both cell-free and transfected cell culture assays. Peptide-conjugated morpholino-oligomers exhibited low toxicity in DBT astrocytoma cells used for culturing MHV. Oligomer administered at micromolar concentrations was delivered to >80% of cells and inhibited virus titers 10-to 100-fold in a sequence-specific and dose-responsive manner. In addition, targeted viral protein synthesis, plaque diameter, and cytopathic effect were significantly reduced. Inhibition of virus infectivity by peptide-conjugated morpholino was comparable to the antiviral activity of the aminoglycoside hygromycin B used at a concentration fivefold higher than the oligomer. These results suggest that this composition of antisense compound has therapeutic potential for control of coronavirus infection.Coronaviruses are medically and economically important pathogens of humans, livestock, and birds. Coronavirus infections cause a wide range of symptoms, including upper respiratory illness, gastroenteritis, myocarditis, nephritis, central nervous system demyelination, encephalitis, hepatitis, and peritonitis, and can have a debilitating effect on the host immune system, leading to secondary microbial infection. In humans a coronavirus infection marked by aerosol transmissibility and a high degree of immunopathology-related mortality has been linked with the outbreak of severe acute respiratory syndrome (52, 64). Other human coronaviruses cause approximately one-third of all cases of common colds (20,29,56

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Antiviral activity of trimeric 2',5'-oligoadenylic acid and some of its analogues

Cited by 2 publications

References 5 publications

2'-5'-Oligoadenylates as a «tool» of innate immunity

2'-5'-Oligoadenylates as a «tool» of innate immunity

Antisense Morpholino-Oligomers Directed against the 5′ End of the Genome Inhibit Coronavirus Proliferation and Growth†

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