Antiviral activity of triterpenoid saponins containing acylated β-amyrin aglycones

Rao, G. Sasibhushana; Sinsheimer, Joseph E.; Cochran, Kenneth W.

doi:10.1002/jps.2600630341

Cited by 48 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…β-amyrin (oleanane-type pentacyclic triterpenoid) was previously reported to exert antiviral efficacies against an influenza A virus (IAV) and herpes simplex virus (HSV) [27]. In our analysis, the percentage of β-amyrin (9.4%) in EGYVIR extract likely supports its anti-viral activity, probably via attenuating the cellular oxidative mechanism.…”

Section: Discussionsupporting

confidence: 52%

EGYVIR: An immunomodulatory herbal extract with potent antiviral activity against SARS-CoV-2

et al. 2020

View full text Add to dashboard Cite

Due to the challenges for developing vaccines in devastating pandemic situations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), developing and screening of novel antiviral agents are peremptorily demanded. Herein, we developed EGYVIR as a potent immunomodulatory herbal extract with promising antiviral activity against SARS-CoV-2. It constitutes of a combination of black pepper extract with curcumin extract. The antiviral effect of EGYVIR extract is attributed to the two key phases of the disease in severe cases. First, the inhibition of the nuclear translocation of NF-kβ p50, attenuating the SARS-CoV-2 infection-associated cytokine storm. Additionally, the EGYVIR extract has an in vitro virucidal effect for SARS-CoV-2. The in vitro study of EGYVIR extract against SARS-CoV-2 on Huh-7 cell lines, revealed the potential role of NF-kβ/TNFα/IL-6 during the infection process. EGYVIR antagonizes the NF-kβ pathway in-silico and in-vitro studies. Consequently, it has the potential to hinder the release of IL-6 and TNFα, decreasing the production of essential cytokines storm elements.

show abstract

Section: Discussionsupporting

confidence: 52%

EGYVIR: An immunomodulatory herbal extract with potent antiviral activity against SARS-CoV-2

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Moreover, the pentacyclic triterpenes are shown to have antiviral properties against HSV (Heidary et al, 2014, Joycharat et al, 2008, Tanaka et al, 2004) and HIV (Cichewicz and Kouzi, 2004), the genetically close viruses to HBV. Notably, β-amyrin (oleanane-type pentacyclic triterpenoid) is shown to have antiviral efficacies against influenza A and HSV (Rao et al, 1974). In our analysis, the high quantity of β-amyrin (20.64 μg/mg) in antioxidant GSLE strongly supports its anti-HBV activity, probably by attenuating the cellular oxidative mechanism.…”

Section: Discussionmentioning

confidence: 99%

Analysis of antioxidative and antiviral biomarkers β-amyrin, β-sitosterol, lupeol, ursolic acid in Guiera senegalensis leaves extract by validated HPTLC methods

Parvez

Alam

Arbab

et al. 2018

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

J.F. Gmel is a broad-spectrum African folk- medicinal plant, having activities against fowlpox and herpes viruses. Very recently, we have shown the anti-hepatitis B vius (HBV) potential of leaves extract (GSLE). Here, we report the antioxidative and hepatoprotective efficacy of GSLE, including HPTLC quantification of four biomarkers of known antioxidative and antiviral activities. In cultured liver cells (HuH7) GSLE attenuated DCFH-induced oxidative stress and cytotoxicity. This was supported by DPPH radical-scavenging and β-carotene-linoleic acid bleaching assays that showed strong antioxidant activity of GSLE. Further, two simple and sensitive HPTLC methods (I and II) were developed and validated to quantify β-amyrin, β- sitosterol, lupeol, ursolic acid in GSLE. While HPTLC-I (hexane: ethylacetate; 75:25; v/v) enabled quantification of β-amyrin (Rf = 0.39; 20.64 μg/mg) and β-sitosterol (Rf = 0.25; 18.56 μg/mg), HPTLC-II (chloroform: methanol; 97:3; v/v) allowed estimation of lupeol (Rf = 0.47; 6.72 μg/mg) and ursolic acid (Rf = 0.23; 5.81 μg/mg) in GSLE. Taken together, the identified biomarkers strongly supported the antioxidant and anti-HBV potential of GSLE, suggesting its activity via abating the oxidative stress. To our knowledge, this is the first report on HPTLC analysis of these biomarkers in that could be adopted for standardization and quality-control of herbal-formulations.

show abstract

“…It has been suggested that the saponins are not directly virucidal but that the antiviral activity involves an inhibition of virus-host cell interaction and subsequent attachment (Amoros et al, 1988; Roner et al, 2007; Roner et al, 2010). Viral inactivation by saponins has also been suggested to involve interactions with membrane glycoproteins following the observation that no direct virucidal effect is seen on the nonenveloped poliovirus (Rao and Sinsheimer, 1974; Ushio and Abe, 1992). It is believed that saponins offer more than one novel mechanism of antiviral action, including interactions with viral envelopes leading to their destruction, interactions with host-cell membranes leading to a loss of virus binding sites and coating of cells to prevent virus binding (Apers et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Characterization of in vivo anti-rotavirus activities of saponin extracts from Quillaja saponaria Molina

Tam

Roner

2011

Antiviral Research

View full text Add to dashboard Cite

Rotavirus is the leading cause of severe diarrhea disease in newborns and young children worldwide with approximately 300,000 pre-adolescent deaths each year. Quillaja saponins are a natural aqueous extract obtained from the Chilean soapbark tree. The extract is approved for use in humans by the FDA for use in beverages as a food addictive. We have demonstrated that Quillaja extracts have strong antiviral activities in vitro against six different viruses. In this study, we evaluated the in vivo antiviral activity of these extracts against rhesus rotavirus (RRV) using a mouse model. We established that at a dosage of 0.015 mg/mouse of saponin extract, RRV induced diarrhea can be significantly reduced from 79% to 11% when mice are exposed to 500 plaque-forming-units (PFU) for each of five consecutive days. Additionally, while a reduction of RRV induced diarrhea depended both on the concentration of virus introduced and on the amount of Quillaja extract given to each mouse, the severity and interval of diarrhea under a variety of conditions tested, in all the treated mice were greatly reduced when compared to those that did not receive the Quillaja extracts. Mechanistically, there is strong evidence that the Quillaja extracts are able to “block” rotavirus infection by inhibiting virus-host attachment through disruption of cellular membrane proteins and/or virus receptors. We believe that Quillaja extracts have promise as antivirals to reduce rotavirus infection and the severity of the disease in humans.

show abstract

Antiviral activity of triterpenoid saponins containing acylated β-amyrin aglycones

Cited by 48 publications

References 21 publications

EGYVIR: An immunomodulatory herbal extract with potent antiviral activity against SARS-CoV-2

EGYVIR: An immunomodulatory herbal extract with potent antiviral activity against SARS-CoV-2

Analysis of antioxidative and antiviral biomarkers β-amyrin, β-sitosterol, lupeol, ursolic acid in Guiera senegalensis leaves extract by validated HPTLC methods

Characterization of in vivo anti-rotavirus activities of saponin extracts from Quillaja saponaria Molina

Contact Info

Product

Resources

About