Antiviral Effect of Ethanolic Extract of Salvadora Persica (Siwak) on Herpes Simplex Virus Infection.

Abstract

Aims:To investigate the effect of ethanolic extract of Salvadora Persica extract on HSV-1 infection both in vitro and in vivo in the mouse model system. Materials and methods: Ethanolic extract of Salvadora Persica was used at different concentrations. BHK cells that grown in Eagles medium were used for virus isolation and titration using PFU/ml. The effects of different concentrations of Salvadora Persica on viral growth in BHK cells as well as cytolytic activity of HSV-1 were evaluated at different time post infection. The therapeutic efficacy of Salvadora Persica in vivo was studied in mice. Lesions were scored and viral isolation from infected skin and ganglia was titrated on BHK cells. Results: Salvadora Persica inhibited the replication of HSV-1 in BHK cells as well as the cytolytic activity of cell free virus. Topical application of Salvadora Persica on the skin of mice infected with HSV-1 reduced the development of cutaneous lesions and the viral titers in the skin and ganglia were also reduced. Conclusion: The results of this work may be beneficial for the treatment of recurrent oral herpes infections.

Pharmacological Benefits of Miswak Users and Its Impact on COVID-19 Patients – A Review

“…The extract with 5-10% had shown no toxicity on in vitro application 38 and study proves that the Herpes simplex HSV-1 viral lesions and ganglia were reduced in the BHK (Baby Hamster Kidney) cells and in the skin of infected laboratory rats when applied with the 5% of ethanolic extract of Salvadora persica 39 . Also the compound Benzyl isothiocyante acts on the prevention of HSV-1 replication and cell free virus in which the roots of the S.persica is found to be Irrigant 40 .…”

Middle Eastern Plant Extracts: An Alternative to Modern Medicine Problems

“…The plant extract acts on the virus by inactivating the virus particles; this is done by interfering with the adsorption step of the virus to a cell [96]. The Meswak plant stick obtained from Saudi Arabia has exhibited anti-viral property against Herpes simplex virus [97]. The ethanol extract of the plant was tested on baby hamster kidney cells which were infected with Herpes simpex virus.…”

“…Thus, it can be deduced that the ethanol extract of Meswak is able to inhibit the replication of the virus. The compounds which are responsible for the anti-viral activity would be benzyl nitrate and benzylisothiocyanate [97]. Hence, the Meswak plant is also known to be effective anti-viral agent, apart from antibacterial agent.…”

The Battle with COVID-19: Insight on External Intervention and Future Vaccination

“…Therefore, S. persica was started to be tested as a natural weapon against COVID-19 through docking studies. Molecular docking simulation of identified flavonoid compounds resulted in interfering of SARS-CoV2 Mpro 'which is an important key for virus transcription and replication' more than the currently used COVID-19 main protease inhibitor Darunavir [109].…”