Antiviral effect of <i>Phyllanthus nanus</i> ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis

Lam, Wai; Leung, Kam Tong; Law, Patrick Tik Wan; Lee, Simon Ming‐Yuen; Chan, Henry Lik‐Yuen; Fung, Kwok‐Pui; Ooi, Vincent E.C.; Waye, Mary Miu Yee

doi:10.1002/jcb.20611

Cited by 36 publications

(19 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TNF-α has been found to be a very important pathogenic mediator in patients with alcoholic liver disease and viral hepatitis, as well as in many animal liver injury models [27] . Recent research showed that the activation of the annexin A7 (Axn7) gene and the expression of the Axn7-GFP fusion protein could cause a decrease in HBsAg secretion [28] . However, the precise mechanism of the anti-HBV activity of hyperoside needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

In vivo and in vitro antiviral activity of hyperoside extracted from Abelmoschus manihot (L) medik

Yang

Huang

et al. 2007

Acta Pharmacologica Sinica

110

View full text Add to dashboard Cite

Aim: To assess the anti‐hepatitis B virus (HBV) effect of hyperoside extracted from Abelmoschus manihot (L) medik. Methods: The human hepatoma Hep G2.2.15 cell culture system and duck hepatitis B virus (DHBV) infection model were used as in vivo and in vitro models to evaluate the anti‐HBV effects. Results: In the cell model, the 50% toxic concentration of hyperoside was 0.115 g/L; the maximum nontoxic concentration was 0.05 g/L. On the maximum nontoxic concentrations, the inhibition rates of hyperoside on HBeAg and HBsAg in the 2.2.15 cells were 86.41% and 82.27% on d 8, respectively. In the DHBV infection model, the DHBV‐DNA levels decreased significantly in the treatment of 0.05 g‐ kg‐1·d‐1 and 0.10 g·kg‐1·d‐1 dosage groups of hyperoside (P<0.01). The inhibition of the peak of viremia was at the maximum at the dose of 0.10 g‐kg‐1·d‐1 and reached 60.79% on d 10 and 69.78% on d 13, respectively. Conclusion: These results suggested that hyperoside is a strong inhibitor of HBsAg and HBeAg secretion in 2.2.15 cells and DHBV‐DNA levels in the HBV‐infected duck model.

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo and in vitro antiviral activity of hyperoside extracted from Abelmoschus manihot (L) medik

Yang

Huang

et al. 2007

Acta Pharmacologica Sinica

110

View full text Add to dashboard Cite

show abstract

“…We have isolated and identified several chemical extracts and pure compounds from Chinese medicine with anti-hepatoma, anti-multidrug resistant hepatoma and antihepatitis viral effects. [16][17][18][19] Essential oil of Ezhu and it active ingredients have been widely used for treatment of malignant tumors in China. 4,5 Furanodiene, an ingredient from Ezhu oil, has been identified to have protective effects against D-galactosamine/ lipopolysaccharide/TNFa induced liver injury.…”

Section: Introductionmentioning

confidence: 99%

Furanodiene induces G2/M cell cycle arrest and apoptosis through MAPK signaling and mitochondria-caspase pathway in human hepatocellular carcinoma cells

Xiao

Yang²,

Li³

et al. 2007

Cancer Biology & Therapy

View full text Add to dashboard Cite

Furanodiene (C 15 H 20 O), a pure compound isolated from Traditional Chinese medicine, Curcuma wenyujin, named Ezhu in Chinese, which structure was determined on the basis of NMR, MS and UV spectrum. In this study, we attempted to characterize in detail the signaling cascades resulted from furanodiene-induced apoptosis in human hepatoma HepG2 cells. Furanodiene inhibited HepG2 cell growth by causing cell cycle arrest at G 2 /M and inducing apoptosis as evidenced by DNA fragmentation assay. We found that furanodiene induced mitochondrial transmembrane depolarization, release of mitochondrial cytochrome c, activation of caspases-3 and the cleavage of PARP. The furanodiene mediated mitochondria-caspase apoptotic pathway also involved activation of p38 and inhibition of ERK mitogen-activated protein kinase (MAPK) signaling. These results for the first time have identified the biological activity of furanodiene against HepG2 cells and provide rationales for further development of essential oil of Ezhu and its ingredients such as furanodiene on treatment of liver diseases.

show abstract

“…Identification of bioactive components in Angelica sinensis is very important not only for the quality control of crude botanical drugs but also for elucidating the therapeutic principle. With the aim of dissecting the molecular pharmacological actions of active ingredients originating from botanical materials, novel approaches have been adopted [Lam et al, 2006]. Moreover, we have used a new approach combining biomembrane extraction and high performance liquid chromatography to identify ferulic acid (FA), ligustilide, senkyunolide H, and senkyunolide I, which are some potentially bioavailable components in Angelica sinensis [Dong et al, 2005].…”

mentioning

confidence: 99%

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Lam

Lin

Lao

et al. 2007

J of Cellular Biochemistry

Self Cite

110

View full text Add to dashboard Cite

Angiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JNK 1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation.

show abstract

Antiviral effect of Phyllanthus nanus ethanolic extract against hepatitis B virus (HBV) by expression microarray analysis

Cited by 36 publications

References 75 publications

In vivo and in vitro antiviral activity of hyperoside extracted from Abelmoschus manihot (L) medik

In vivo and in vitro antiviral activity of hyperoside extracted from Abelmoschus manihot (L) medik

Furanodiene induces G2/M cell cycle arrest and apoptosis through MAPK signaling and mitochondria-caspase pathway in human hepatocellular carcinoma cells

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Contact Info

Product

Resources

About