Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus

Chai, Weidong; Burwinkel, Michael; Wang, Zhenya; Palissa, Christiane; Esch, Bettina; Twardziok, Sven; Rieger, Jan; Wrede, Paul; Schmidt, Michael F. G.

doi:10.1007/s00705-012-1543-0

Cited by 80 publications

(70 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…; Chai et al . ; Rodríguez‐Díaz and Monedero ), in this case, a decrease in the virus entrance in the cell population could be seen. However, our results suggests that the viruses able to enter the cells were enough to induce the Ca 2+ release as supported by no significant differences in Ca 2+ levels when compared to the positive control (Fig.…”

Section: Discussionmentioning

confidence: 62%

In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production

et al. 2016

View full text Add to dashboard Cite

Aims: The aim of this study was to determine the antiviral activity of four probiotic metabolites (Lactobacillus and Bifidobacetrium species) against rotavirus in vitro infection monitored by the NSP4 protein production and Ca 2+ release. Methods and Results: The antiviral effect of the metabolites was performed due a comparison between a blocking model and an intracelullar model on MA104 cells, with the response of NSP4 production and Ca 2+ liberation measured by flow cytometry. Significant results were obtained with the metabolites of Lactobacillus casei, and Bifidobacterium adolescentis in the reduction of the protein production (P = 0Á04 and P = 0Á014) and Ca 2+ liberation (P = 0Á094 and P = 0Á020) in the intracellular model, which suggests a successful antiviral activity against RV infection. Conclusions: This study demonstrates that probiotic metabolites were able to interfere with the final amount of intracellular NSP4 protein and a successful Ca 2+ regulation, which suggests a new approach to the mechanism exerted by probiotics against the rotavirus infection. Significance and Impact of the Study: A novel anti-rotaviral effect exerted by probiotic metabolites monitored by the NSP4 protein during the RV in vitro infection and the effect on the Ca 2+ release is reported; suggesting a reduction on the impact of the infection by decreasing the damage of the cells preventing the electrolyte loss.

show abstract

Section: Discussionmentioning

confidence: 62%

In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Different concentrations of HDRsEf1 ranging from 1 × 10 4 CFU/mL to 1 × 10 9 CFU/ mL were added to HT-29 cell monolayers in a 96-well plate (Nest Biotech co., Ltd) for 3 h. At the end of the incubation period, the MTT viability assay was carried out as described previously (Meshitsuka et al 1987;Chai et al 2013). The cell survival rate was determined as bacteria average OD value/control average OD value, the non-cytotoxic maximal concentration of HDRsEf1 was selected for continuous assays.…”

Section: Assessment Of Cellular Toxicity Of Hdrsef1mentioning

confidence: 99%

The inflammation regulation effects ofEnterococcus faeciumHDRsEf1 on human enterocyte-like HT-29 cells

Tian

Yang

et al. 2016

Animal Cells and Systems

View full text Add to dashboard Cite

Enterococcus faecium HDRsEf1 strain used as a probiotic to inhibit intestine inflammation and improve animal growth performance has been proved by our research team; however, it remains unclear how HDRsEf1 was recognized by intestine cells and how it activates the downstream pathway which benefit intestine health. In this study, HDRsEf1 was used to stimulate HT-29 cell line to partially uncover the intestine benefit mechanism of HDRsEf1. The results of cell viability assays showed that HDRsEf1 had no toxicity on HT-29 at concentrations up to 1 × 10 8 CFU/mL, HDRsEf1 could upregulate the TLR1, TLR2, and TLR6 mRNA level, especially TLR2, and significantly downregulate the mRNA level of TLR4, TLR5, TLR7, TLR8, but did not significantly affect the mRNA or protein level of MyD88, which suggests that HDRsEf1 activates the TLR2 pathway in an MyD88-independent pattern. HDRsEf1 could significantly downregulate the mRNA level of pro-inflammatory factors IL-1β, IL-6, IL-8, IL-12p35, IL-17, and TNF-α and did not affect the anti-inflammatory factors IL-10, PPAR-γ, and TSLP; besides HDRsEf1 did not upregulate the degradation of IκB in HT-29 cells. By contrast, enterohemorrhagic E. coli (EHEC) O157:H7 strongly up-regulated the mRNA level of pro-inflammatory factors IL-1β, IL-6, IL-8, IL-23, and TNF-α, downregulated obviously anti-inflammatory factor PPAR-ɣ, and obviously upregulated the degradation of IκB, which suggested that HDRsEf1 may act as an antagonist to regulate intestine inflammation response to intestine pathogen. These findings shed a light on the intestine benefit mechanism of HDRsEf1. ARTICLE HISTORY

show abstract

“…In this case, four protocols were studied, including pretreatment of cells, competition, postinfection treatment, and cell-free preincubation. In all four cases, an antiviral dose-response effect was observed (Chai et al, 2013).…”

Section: Efficacy Of Probiotics: In Vitro Datamentioning

confidence: 79%

Intestinal Microbiota and Susceptibility to Viral Infections

Monedero

Rodrı́guez-Dı́az

2016

Probiotics, Prebiotics, and Synbiotics

View full text Add to dashboard Cite

Antiviral effects of a probiotic Enterococcus faecium strain against transmissible gastroenteritis coronavirus

Cited by 80 publications

References 35 publications

In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production

In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production

The inflammation regulation effects ofEnterococcus faeciumHDRsEf1 on human enterocyte-like HT-29 cells

Intestinal Microbiota and Susceptibility to Viral Infections

Contact Info

Product

Resources

About