In vitro antiviral activity of Lactobacillus casei and Bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production

Abstract

Aims: The aim of this study was to determine the antiviral activity of four probiotic metabolites (Lactobacillus and Bifidobacetrium species) against rotavirus in vitro infection monitored by the NSP4 protein production and Ca 2+ release. Methods and Results: The antiviral effect of the metabolites was performed due a comparison between a blocking model and an intracelullar model on MA104 cells, with the response of NSP4 production and Ca 2+ liberation measured by flow cytometry. Significant results were obtained with the metabolites of Lactobacillus casei, and Bifidobacterium adolescentis in the reduction of the protein production (P = 0Á04 and P = 0Á014) and Ca 2+ liberation (P = 0Á094 and P = 0Á020) in the intracellular model, which suggests a successful antiviral activity against RV infection. Conclusions: This study demonstrates that probiotic metabolites were able to interfere with the final amount of intracellular NSP4 protein and a successful Ca 2+ regulation, which suggests a new approach to the mechanism exerted by probiotics against the rotavirus infection. Significance and Impact of the Study: A novel anti-rotaviral effect exerted by probiotic metabolites monitored by the NSP4 protein during the RV in vitro infection and the effect on the Ca 2+ release is reported; suggesting a reduction on the impact of the infection by decreasing the damage of the cells preventing the electrolyte loss.

Modulation of rotavirus severe gastroenteritis by the combination of probiotics and prebiotics

“…The activity of probiotics and prebiotics against RV pathogenesis may be attributable to decreased viral shedding possibly due to the interaction of probiotics (or their metabolites) and prebiotics with the viral particles avoiding the entry into enterocytes and as a consequence reducing the RV replication (Rigo-Adrover et al 2017 ). Moreover, the in vitro effect of metabolites of Lactobacillus casei , and Bifidobacterium adolescentis was associated with a reduced expression of the RV enterotoxin NSP4 and reduced levels of Ca 2+ liberation suggesting that cell will not reach the electrolyte imbalance caused by this pathway (Olaya Galán et al 2016 ). On the other hand, the modulation of RV immune response by probiotics and prebiotics has been associated with a generalized antiviral response via pattern recognition receptor signaling and through promoting type I IFNs, which are key regulators of IFN signaling pathway (Ishizuka et al 2016 ; Kang et al 2015 ).…”

Strain-Specific Probiotic Properties of Bifidobacteria and Lactobacilli for the Prevention of Diarrhea Caused by Rotavirus in a Preclinical Model

“…However, before moving to clinical trials, the safety and efficacy of probiotics need to be proven in preclinical models. In this sense, preclinical evidence has been accumulated for the following species: Lactobacillus casei [32][33][34], Lactobacillus plantarum [31], Lactobacillus rhamnosus GG [5,14,26,35], Bifidobacterium adolescentis [34], Bifidobacterium bifidum [29], Bifidobacterium infantis [29], Bifidobacterium breve [13,36], and Bifidobacterium lactis [35]. In addition, these previous studies led to the further study of the clinical evidence for the following species: Lactobacillus acidophilus [16,20,32,37], L. casei [26], Lactobacillus reuteri [38,39], L. rhamnosus GG [16,17,20,22,[40][41][42][43], Lactobacillus sporogenes [44,45], B. lactis [46][47][48], and Bifidobacterium longum [16,20,37].…”

Bifidobacterium adolescentis (DSM 20083) and Lactobacillus casei (Lafti L26-DSL): Probiotics Able to Block the In Vitro Adherence of Rotavirus in MA104 Cells

“…Some studies have demonstrated the beneficial effect of probiotics against rotavirus infection [ 9 , 10 ]. Most of them are clinical assays proving that the use of probiotics can lessen severity and duration of rotavirus diarrhea [ 11 ], whereas other studies are performed in vitro directed to the understanding of molecular and biochemical pathways associated with the mechanism employed by probiotics to accomplish the antiviral activity [ 12 – 14 ].…”