Antiviral peptides from marine gorgonian-derived fungus Aspergillus sp. SCSIO 41501

Jia

et al. 2020

Virol J

Self Cite

Background: Herpes simplex virus 1, an enveloped DNA virus belonging to the Herpesviridae family, spreads to neurons and causes pathological changes in the central nervous system. The purpose of this study was to investigate the potency and mechanism of antiviral activity of Aspergillipeptide D, a cyclic pentapeptide isolated from a culture broth of marine gorgonian-derived fungus Aspergillus sp. SCSIO 41501, At present, there are many studies on the anti-tumor, anti-clotting, anti-oxidant and immunoinflammatory effects of Aspergillipeptide D, but little research has been done on the anti-HSV-1 activity of Aspergillipeptide D. Methods: The anti-HSV-1 activity of Aspergillipeptide D was evaluated by plaque reduction assay. The mechanism of action against HSV-1 was determined from the effective stage. Then we assayed the viral DNA replication, viral RNA synthesis and protein expression, respectively. We also identified the proteins that interact with gB by mass spectrometry, and assayed the effect of Aspergillipeptide D on the interaction between the virus gB protein and cell proteins. Results: Plaque reduction experiments showed that Aspergillipeptide D did not affect HSV-1 early infection events, including viral inactivation, attachment and penetration. Interestingly, Aspergillipeptide D dramatically reduced both the gene and protein levels of viral late protein gB, and suppressed its location in the endoplasmic reticulum and Golgi apparatus. In contrast, overexpression of gB restored viral production. Finally, proteomic analysis revealed that the numbers of cellular proteins that interacted with gB protein was largely decreased by Aspergillipeptide D. These results suggested that Aspergillipeptide D inhibited gB function to affect HSV-1 intercellular spread. Conclusions: Our results indicated that Aspergillipeptide D might be a potential candidate for HSV-1 therapy, especially for ACV-resistant strains.

Section: Chemicals and Reagentsmentioning

confidence: 99%

“…SCSIO 41501 as white solid with the molecular formula of C40H49N5O8 ( Fig. 1a) [14]. In this study, we further investigated the potency and mechanism of antiviral activity of Aspergillipeptide D against HSV-1 and ACV-resistant strains.…”

Section: Introductionmentioning

confidence: 99%

Anti-HSV-1 activity of Aspergillipeptide D, a cyclic pentapeptide isolated from fungus Aspergillus sp. SCSIO 41501

Jia

et al. 2020

Virol J

Self Cite

Medicinal Research Reviews

“…Aspergillipeptide D (Figure A), a cyclic pentapeptide, was produced by marine gorgonian‐derived fungus Aspergillus sp. SCSIO 41501 . It exhibited good antiviral activity against herpes simplex virus type 1 (HSV‐1) under its non‐cytotoxic concentration against a Vero cell line (IC 50 = 9.5 µM).…”

Section: Classification and Examples Of Natural Bioactive Cyclopeptidesmentioning

confidence: 99%

A gold mine for drug discovery: Strategies to develop cyclic peptides into therapies

Jing

Jin

2019

121

130

As a versatile therapeutic modality, peptides attract much attention because of their great binding affinity, low toxicity, and the capability of targeting traditionally “undruggable” protein surfaces. However, the deficiency of cell permeability and metabolic stability always limits the success of in vitro bioactive peptides as drug candidates. Peptide macrocyclization is one of the most established strategies to overcome these limitations. Over the past decades, more than 40 cyclic peptide drugs have been clinically approved, the vast majority of which are derived from natural products. The de novo discovered cyclic peptides on the basis of rational design and in vitro evolution, have also enabled the binding with targets for which nature provides no solutions. The current review summarizes different classes of cyclic peptides with diverse biological activities, and presents an overview of various approaches to develop cyclic peptide‐based drug candidates, drawing upon series of examples to illustrate each strategy.

“…Compound 1 was isolated from a culture broth of a gorgonian-originating fungal strain, A. sp. SCSIO 41501, and then characterized as a new linear peptide with three amino acid residues, d-Tyr, d-Val, and l-Trp [23]. Compound 2 was obtained from the coral-associated fungus A. terreus, whose structure featured an unusual (E)-oxime group, which is rare in natural products [24].…”

Section: Indole Alkaloidsmentioning

confidence: 99%

“…To the best of our knowledge, an overwhelming majority of these fungal strains were isolated from marine invertebrates, including corals, sponges, crabs, shrimps, ascidians, and some isopods. For instance, a total of 24 heterocyclic alkaloids (1, 2, 20, 68-72, 83, 105, 106, 108, 112, 113, 116-124, and 128) were identified from the endophytic fungal strains of marine gorgonian species, including Melitodes squamata [23], Sarcophyton subviride [24], Pseudopterogorgia sp. LZD-14 [33], Muricella flexuosa [49], Echinogorgia aurantiaca [59], Carijoa sp.…”

Section: Production Environmentmentioning

confidence: 99%

Recent Discovery of Heterocyclic Alkaloids from Marine-Derived Aspergillus Species

Yuan

et al. 2020

Marine Drugs

Nitrogen heterocycles have drawn considerable attention due to of their significant biological activities. The marine fungi residing in extreme environments are among the richest sources of these basic nitrogen-containing secondary metabolites. As one of the most well-known universal groups of filamentous fungi, marine-derived Aspergillus species produce a large number of structurally unique heterocyclic alkaloids. This review attempts to provide a comprehensive summary of the structural diversity and biological activities of heterocyclic alkaloids that are produced by marine-derived Aspergillus species. Herein, a total of 130 such structures that were reported from the beginning of 2014 through the end of 2018 are included, and 75 references are cited in this review, which will benefit future drug development and innovation.