Background: The COVID-19 epidemic has been outbreak and even spread to the global. The whole medical system in the world is facing great challenges. As one of the main forces, nursing staff are at the highest risk. Their negative emotions and job burnout are worthy of attention. This study aims to investigate the status of burnout and anxiety among nurses during COVID-19 epidemic and analyze the influencing factors of burnout.Methods: A cross-sectional survey was conducted from February 19 to February 25, 2020. A total of 1011 nurses from Wuhan tertiary hospitals were distributed with the basic information questionnaire, Maslach Burnout Inventory - General Survey (MBI-GS) and State-Trait anxiety inventory (STAI). The number of final valid questionnaire was 885. The effective recovery rate was 87.5%. Linear regression analysis was performed to explore the influencing factors.Results: The mean score of three dimensions of MBI-GS was 11.50, 6.02, 24.47, respectively. The mean score of state anxiety was 45.52 and trait anxiety, 43.78. Anxiety was positively related to emotional exhaustion and cynicism, and negatively to personal accomplishment. The positive factors of burnout were personnel agency, 5 years or less work experience, living in hospital dormitory, Wuhan medical team, working more than 9 hours, and the best level of knowledge of COVID-19. And having no siblings, intermediate title, working in isolation wards, 3 and more night shifts per week, living in hotels, and having confirmed or suspected medical staff around were negative factors. Conclusions: From this study, the anxiety level among nurses during the COVID-19 is serious, however, the level of burnout is mild to medium. Managers should continue to pay attention to the negative emotions of nurses and related factors, and take interventions timely to stabilize the nursing team.