Anxiolytic-like effect of Chlorpheniramine in inhibitory avoidance in goldfish submitted to telencephalic ablation

Faganello, Flávia Roberta; Mattioli, Rosana

doi:10.1016/j.pnpbp.2006.06.015

Cited by 31 publications

(31 citation statements)

References 31 publications

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“…The anxiolytic effect of diazepam was seen in a dose range that did not affect swimming speed. Recently, diazepam has been found to have anxiolytic effects in another species of fish, goldfish [12]. In that study, lesions of the telencephalon did not alter the anxiolytic effect suggesting a more caudal locus of the diazepam effect, possibly in the diencephalon.…”

Section: Discussionmentioning

confidence: 80%

Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety

Bencan

Sledge

Levin

2009

Pharmacology Biochemistry and Behavior

367

290

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Zebrafish are becoming more widely used to study neurobehavioral pharmacology. We have developed a method to assess novel environment diving behavior of zebrafish as a model of stress response and anxiolytic drug effects. In a novel tank, zebrafish dwell in the bottom of the tank initially and then increase their swimming exploration to higher levels as the session progresses. We previously found that nicotine, which has anxiolytic effects in rodents and humans, significantly lessens the novel tank diving response in zebrafish. The specificity of the diving effect was validated with a novel vs. non-novel test tank. The novel tank diving response zebrafish was tested when given three anxiolytic drugs from two different chemical and pharmacological classes: buspirone, chlordiazepoxide and diazepam. When the test tank was novel the diving response was clearly seen where as it was significantly reduced when the test tank was not novel. Buspirone, a serotonergic (5HT 1A receptor agonist) anxiolytic drug with some D 2 dopaminergic effect, had a pronounced anxiolytic-like effect in the zebrafish diving model at doses that did not have sedative effects. In contrast, chlordiazepoxide, a benzodiazepine anxiolytic drug, which is an effective agonist at GABA-A receptors, did not produce signs of anxiolysis in zebrafish over a broad dose range up to those that caused sedation. Diazepam another benzodiazepine anxiolytic drug did produce an anxiolytic effect at doses that did not cause sedation. The zebrafish novel tank diving task can be useful in discriminating anxiolytic drugs of several classes (serotonergic, benzodiazepines and nicotinic).

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Section: Discussionmentioning

confidence: 80%

Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety

Bencan

Sledge

Levin

2009

Pharmacology Biochemistry and Behavior

367

290

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“…Previous studies suggested an innate preference of teleost fish for dark environments (9). In studies in which the same model of inhibitory avoidance task was used, fish took longer to leave the white compartment of the aquarium after experiencing aversive stimulus immediately after crossing to the dark compartment (7,8,11). These data suggest both the effectiveness of the model proposed and the adequacy of the aversive stimulus used.…”

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confidence: 91%

Chlorpheniramine facilitates inhibitory avoidance in teleosts submitted to telencephalic ablation

Faganello

Mattioli

2008

Braz J Med Biol Res

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The present study investigated the involvement of H(1) histaminegic receptor on the acquisition of inhibitory avoidance in Carassius auratus submitted to telencephalic ablation. The fish were submitted to telencephalic ablation 5 days before the experiment. The inhibitory avoidance procedure included 1 day for habituation, 3 days for training composed of 3 trials each (1st day: T1, T2, T3; 2nd day: 2T1, 2T2, 2T3; 3rd day: 3T1, 3T2, 3T3) and 1 day for test. On training days, the fish were placed in a white compartment, after 30 s the door was opened. When the fish crossed to a black compartment, a weight was dropped (aversive stimuli). Immediately after the third trial, on training days, the fish received, intraperitoneally, one of the pharmacological treatments (saline (N = 20), 8 (N = 12) or 16 (N = 13) µg/g chlorpheniramine, CPA). On the test day, the time to cross to the black compartment was determined. The latency of the saline group increased significantly only on the 3rd trial of the 2nd training day (mean ± SEM, T1 (50.40 ± 11.69), 2T3 (226.05 ± 25.01); ANOVA: P = 0.0249, Dunn test: P < 0.05). The group that received 8 µg/g CPA showed increased latencies from the 2nd training day until the test day (T1 (53.08 ± 17.17), 2T2 (197.75 ± 35.02), test (220.08 ± 30.98); ANOVA: P = 0.0022, Dunn test: P < 0.05)). These results indicate that CPA had a facilitating effect on memory. We suggest that the fish submitted to telencephalic ablation were able to learn due to the local circuits of the mesencephalon and/or diencephalon and that CPA interferes in these circuits, probably due an anxiolytic-like effect.

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“…Some studies describe the inhibitory effects of HA on learning and memory processes (10,11), while others have provided evidence that HA plays a role in reinforcement and mnemonic processes (12,13). A relationship between the NHS and anxiety has been suggested in studies of the behavior of fish (3,14) and rodents (15,16). We have reported that chlorpheniramine, a histaminergic H 1 receptor antagonist, modulates some components of emotional learning in fish (3).…”

Section: Introductionmentioning

confidence: 84%

“…A relationship between the NHS and anxiety has been suggested in studies of the behavior of fish (3,14) and rodents (15,16). We have reported that chlorpheniramine, a histaminergic H 1 receptor antagonist, modulates some components of emotional learning in fish (3). Kamei and Tasaka (17) showed that the intracerebroventricular (icv) injection of HA and L-histidine (LH) prior to the test caused a significant reduction of the latency response in old rats in an active avoidance response test, therefore facilitating the memory processes.…”

Section: Introductionmentioning

confidence: 99%

L-histidine provokes a state-dependent memory retrieval deficit in mice re-exposed to the elevated plus-maze

Serafim

Kishi

Canto-de-Souza

et al. 2010

Braz J Med Biol Res

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0.05, Kruskal-Wallis test), or at the dose of 500 mg/kg (OAE: 5.27 ± 0.73, 4.87 ± 0.66; OAT: 63.93 ± 11.72, 63.58 ± 10.22; P > 0.05, Fisher LSD test). At T2, LH-LH animals did not reduce open-arm activity (OAE and OAT) at the dose of 200 mg/kg (T1: 4.87 ± 0.66, T2: 5.47 ± 1.05; T1: 63.58 ± 10.22; T2: 49.01 ± 8.43 for OAE and OAT, respectively; P > 0.05, Wilcoxon test) or at the dose of 500 mg/kg (T1: 4.80 ± 1.60, T2: 4.70 ± 1.04; T1: 51.55 ± 12.10, T2: 43.88 ± 10.64 for OAE and OAT, respectively; P > 0.05, Fisher LSD test), showing an inability to evoke memory 24 h later. These data suggest that LH does not act on anxiety but does induce a state-dependent memory retrieval deficit in mice.]]>

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Anxiolytic-like effect of Chlorpheniramine in inhibitory avoidance in goldfish submitted to telencephalic ablation

Cited by 31 publications

References 31 publications

Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety

Buspirone, chlordiazepoxide and diazepam effects in a zebrafish model of anxiety

Chlorpheniramine facilitates inhibitory avoidance in teleosts submitted to telencephalic ablation

L-histidine provokes a state-dependent memory retrieval deficit in mice re-exposed to the elevated plus-maze

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