Apelin Effects Migration and Invasion Abilities of Colon Cancer Cells

Podgórska, Marta; Pietraszek-Gremplewicz, Katarzyna; Nowak, Dorota

doi:10.3390/cells7080113

Cited by 24 publications

(15 citation statements)

References 55 publications

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“…Our previous study showed that apelin added to culture medium stimulates proliferation, migration and invasion of colon cancer cell lines, connected with increased ability to migratory protrusions formation, metalloproteases secretion, and cytoskeleton rearrangement [24]. According to our knowledge, this is the first study focusing on mRNA and protein level of apelin and its receptor in primary colorectal carcinoma patients.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Apelin and Apelin Receptor Level in the Primary Tumor and Serum of Colorectal Cancer Patients

Podgórska

Diakowska

Pietraszek-Gremplewicz

et al. 2019

JCM

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Colorectal cancer is the second deadliest tumor, which has a positive correlation with obesity which led to increasing interest in the relationship between adipokines and cancer progression. Apelin is a secreted peptide involved in regulation of tumor progression and invasiveness. In this study, we examined apelin and apelin receptor expression level in colorectal cancer. Apelin, and its receptor mRNA, and protein expression levels were measured in tumor tissue of 56 surgically treated colorectal adenocarcinoma (CRC) patients. We also analyzed apelin and apelin receptor protein levels in sera of 56 CRC patients and 27 healthy controls. The mRNA and protein level of this peptide and its receptor was higher in tumors than that in control tissue. Serum levels of apelin and apelin receptor were increased in CRC patients in comparison to controls. The concentration of serum apelin level significantly increased in individuals with lymph node and distant metastasis. Obtained results suggest that apelin could be an important factor in progression of colorectal carcinoma.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Apelin and Apelin Receptor Level in the Primary Tumor and Serum of Colorectal Cancer Patients

Podgórska

Diakowska

Pietraszek-Gremplewicz

et al. 2019

JCM

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“…LS180 proliferation was inhibited by blocking either APJ or Notch3, suggesting that apelin-13/APJ could promote colorectal cancer cell proliferation by modulating Notch3 pathways (45). In a study by Podgoŕska et al, four apelin peptides, including [Pyr1] apelin-13, apelin-13, apelin-17, and apelin-36, could promote colon cancer cells migration and invasion (46).…”

Section: Colon Cancermentioning

confidence: 99%

Study Progression of Apelin/APJ Signaling and Apela in Different Types of Cancer

Liu

Lü

et al. 2021

Front. Oncol.

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Apelin is an endogenous ligand that binds to the G protein-coupled receptor angiotensin-like-receptor 1 (APJ). Apelin and APJ are widely distributed in organs and tissues and are involved in multiple physiological and pathological processes including cardiovascular regulation, neuroendocrine stress response, energy metabolism, etc. Additionally, apelin/APJ axis was found to play an important role in cancer development and progression. Apela is a newly identified endogenous ligand for APJ. Several studies have revealed the potential role of Apela in cancers. In this article, we review the current studies focusing on the role of apelin/APJ signaling and Apela in different cancers. Potential mechanisms by which apelin/APJ and Apela mediate the regulation of cancer development and progression were also mentioned. The Apelin/APJ signaling and Apela may serve as potential therapeutic candidates for treatment of cancer.

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“…Furthermore, we demonstrated that exogenous apelin added to the culture medium increases the migration and invasion abilities of colon cancer cells. Several apelin peptides were used to show that this adipokine stimulates the migratory protrusion formation, as well as proteolytic activity of the examined cells (17). Moreover, several other studies have examined the effect of apelin on cancer cell biology, by adding recombinant apelin to the culture medium (14,(18)(19)(20)(21), omitting the fact that cancer cells also express and secrete this adipokine.…”

mentioning

confidence: 99%

The Role of Apelin and Apelin Receptor Expression in Migration and Invasiveness of Colon Cancer Cells

Podgórska

Pietraszek-Gremplewicz

Olszańska

et al. 2021

Anticancer Res

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Background/Aim: Colon cancer is the second deadliest malignancy for human. Its correlation with obesity has led to an increasing number of studies focusing on the role of adipokines in colon cancer development. Apelin, which belongs to the family of adipokines, affects several pathological processes, including heart diseases, obesity and carcinogenesis. In this study, we examined the importance of apelin and apelin receptor (APJ) during motility regulation of colon cancer cells. Materials and Methods: Colon cancer cells with overexpression of apelin receptor, as well as cells with down-regulation of apelin were used in this study. Migration and invasion ability was tested using Transwell ® filters. The proteolytic activity was analyzed with fluorescentsubstrate degradation assay and gelatin zymography. We also used confocal microscopy to examine migratory protrusion formation and the localization of MT1-MMP. The levels of AKT and ERK kinases were evaluated using Western blotting assay. Results: Overexpression of APJ receptor resulted in increased migration and invasion abilities through stimulation of migratory protrusion formation and proteolytic activity. These processes were mediated by PI3K/AKT and MAPK signaling pathways. Opposite effect was obtained when the level of apelin was down-regulated. Conclusion: The level of apelin and its receptor is strictly connected with regulation of migration and invasion of colon cancer cells. Therefore, apelinergic system seems to be a promising target for anti-cancer therapy.Colorectal cancer (CRC) is the second deadliest malignancy for all the human population. The lifetime risk of developing CRC is about 1 in 23 for men and 1 in 25 women, while it has been shown that it is strongly associated with environmental and lifestyle factors, such as diet, tobacco, alcohol, androgen deprivation therapy, and obesity (1). A meta-analysis showed that elevated body mass index (BMI) is positively correlated with CRC development. There are several evidences for this association including changes in insulin-like growth factor 1 signaling, sex hormones, systemic inflammation and altered expression of adipokines (2).Adipose tissue not only regulates energy homeostasis, but is also a part of endocrine system secreting adipokines, growth factors, cytokines and chemokines. Adipokines are essential mediators of various metabolic processes, such as gluconeogenesis, glucose uptake, insulin signaling and fatty acid oxidation. During the development of obesity, the excess of adipose tissue results in increased adipokines secretion, that lead to protection against some of the adverse metabolic consequences of obesity (3). However, several studies have shown that increased level of adipokines, such as apelin, could be connected with higher probability to develop cancer, including gastroesophageal cell carcinoma (4), gastric cancer (5), non-small cell lung carcinoma (6), bladder cancer (7), prostate cancer (8), ovarian cancer (9), and breast cancer (10).The apelin-encoding gene (APLN) is located on...

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Apelin Effects Migration and Invasion Abilities of Colon Cancer Cells

Cited by 24 publications

References 55 publications

Evaluation of Apelin and Apelin Receptor Level in the Primary Tumor and Serum of Colorectal Cancer Patients

Evaluation of Apelin and Apelin Receptor Level in the Primary Tumor and Serum of Colorectal Cancer Patients

Study Progression of Apelin/APJ Signaling and Apela in Different Types of Cancer

The Role of Apelin and Apelin Receptor Expression in Migration and Invasiveness of Colon Cancer Cells

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