Apheresis of low density lipoproteins using a heparin-based sorbent with low antithrombin III binding capacity

Sinitsyn, V. V.; Mamontova, Anna G.; Konovalov, Gennady A.; Кухарчук, В. В.

doi:10.1016/0021-9150(90)90008-7

Cited by 3 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The technique involves passing whole blood or plasma of the patient through a cartridge filled with an adsorbent which can easily adsorb the toxin molecules, see Figure 1 a,b. According to selectivity, generally adsorbents can be classified as broad spectrum, affinity adsorbents and immuno-adsorbents, of which the latter has the highest selectivity [2][3][4][5]. Materials, most commonly used are activated charcoal [6], porous resins and fibers.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

Wang¹,

Yu²

2013

Cellulose - Medical, Pharmaceutical and Electronic Applications

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

Wang¹,

Yu²

2013

Cellulose - Medical, Pharmaceutical and Electronic Applications

View full text Add to dashboard Cite

Aptamer-Based Affinity Chromatography for Protein Extraction and Purification

Perret

Boschetti²

2019

Advances in Biochemical Engineering/Biotechnology

View full text Add to dashboard Cite

C3a and C5a Anaphylatoxins Bind to Heparin‐Based Sorbent in Low Density Lipoprotein Apheresis: In Vitro and In Vivo Investigations

et al. 1992

View full text Add to dashboard Cite

Plasmasorption on a heparin-based sorbent was performed in vitro. It demonstrated affinity of the C3a and C5a anaphylatoxins for the sorbent: C3a was removed almost completely (97%), and the C5a concentration decreased on average by 55%. The plasma level of C3a and C5a complement components was also monitored during the procedure of clinical extracorporeal low density lipoprotein (LDL) apheresis on the sorbent in patients with familial hypercholesterolemia. A two- to threefold increase in C3a (up to 1,500 ng/ml) was observed after plasma separation by the IBM 2997 cell sorter. Subsequent processing of the plasma through the column led to the low level of C3a detected (less than 50 ng/ml), demonstrating significant uptake of C3a by the sorbent column. The removed C3a was found in the eluate obtained after regeneration of the sorbent with 2 M NaCl solution. No significant increase in C5a was found during the procedure. Nevertheless, some C5a was detected in the eluate from the sorbent. The content of C3a and C5a in patients blood after the treatment was approximately the same as it was initially, 200-500 ng/ml for C3a and less than 10 ng/ml for C5a. The removal of C3a and C5a anaphylatoxins by heparin-based sorbent should be regarded as an advantage of this type of plasmasorbent.

show abstract

Apheresis of low density lipoproteins using a heparin-based sorbent with low antithrombin III binding capacity

Cited by 3 publications

References 13 publications

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

Bioactive Bead Type Cellulosic Adsorbent for Blood Purification

Aptamer-Based Affinity Chromatography for Protein Extraction and Purification

C3a and C5a Anaphylatoxins Bind to Heparin‐Based Sorbent in Low Density Lipoprotein Apheresis: In Vitro and In Vivo Investigations

Contact Info

Product

Resources

About