Apical sealing ability of chitosan nanoparticles in epoxy-resin-based endodontic sealer

Abstract

A good sealing ability of apical part of root canal system is needed to prevent microleakage thus resulting long-lasting successful treatment. Chitosan nanoparticles as nanofillers could be added to epoxy resin sealer to increase adaptation to dentinal wall. The purpose of this study was to evaluate the apical sealing ability of chitosan nanoparticles addition to epoxy-resin-based sealer. Thirty mandibular premolars were used in this study. The root canals were prepared using rotary files up to #30/0.09. The canal was irrigated with 2.5% NaOCl and 17% EDTA, then was rinsed with sterile water. All teeth were randomly divided into 3 groups (n=10). Group I was obturated with gutta-percha, Group II was obturated with gutta-percha and epoxy resin sealer, group III was obturated with gutta-percha and epoxy resin sealer that was added with chitosan nanoparticles. All specimens were stored in an incubator for 7 days at 37 °C. After that, specimens were tested with centrifuging dye penetration method with methylene blue 2% solution. The specimens were longitudinally sectioned, observed under stereomicroscope (8x magnification) and measured in millimeters (mm). The data were analyzed using one-way Anova. The results of one-way Anova showed that dye penetration in apical part of the root canal in all groups was statistically significant difference. The addition of chitosan nanoparticles to epoxy resin sealer increases the apical sealing ability of root canal obturation material.

<p>Analisis perbedaan radiopasitas antara sealer berbahan dasar resin epoksi, mineral trioxide aggregate (MTA), dan biokeramik dengan menggunakan densitometer pada radiografi periapikal</p><p>Analysis of radiopacity differences between sealers based on epoxy resin, mineral trioxide aggregate (MTA), and bioceramics on periapical radiographs using a densitometer</p>

“…12 Sealer yang digunakan dalam perawatan endodontik dibagi menjadi beberapa bahan dasar antara lain sealer berbahan dasar seng oksida eugenol, kalsium hidroksida, ionomer kaca, resin epoksi, Mineral Trioxide Aggregate (MTA), dan Biokeramik. 13,14 Radiopasitas suatu sealer dipengaruhi oleh komposisi bahan kimia, media kontras anorganik, nomor atom, komposisi radiopacifier dan densitas pada sealer. Sealer berbahan dasar resin epoksi merupakan sealer saluran akar yang memiliki sifat radiopak, kelarutan rendah, penyusutan kecil, memiliki sifat antibakteri, dan toksisitas rendah.…”

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

“…Previous studies have shown that the addition of chitosan nanoparticles to ERB sealer did not affect the physical properties of ERB sealer, which exhibited acceptable sealer for obturation material. [11][12][13] However, until now there is deliberation about the appropriate concentration of chitosan nanoparticles, which should be added to sealer. Thus, the purpose of this study was to evaluate the addition of nanoparticle chitosan with concentrations of 0, 10, 20, and 30% to the ERB sealer on its antibacterial and cytotoxicity effect.…”

“…Previous studies have shown that the addition of chitosan nanoparticles to ERB sealer did not affect the physical properties of ERB sealer, which exhibited acceptable sealer for obturation material. 11 12 13 …”