Apigenin attenuates hippocampal oxidative events, inflammation and pathological alterations in rats fed high fat, fructose diet

Kalivarathan, Jagan; Chandrasekaran, Sathiya Priya; Kalpana, K; Vidhya, R.; Anuradha, Carani Venkatraman

doi:10.1016/j.biopha.2017.01.162

Cited by 33 publications

(25 citation statements)

References 26 publications

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“…Previous findings have registered that HFFD feeding to animals cause decreased activity enzymatic (SOD, CAT and GPx) and non-enzymatic (GSH) antioxidants and increased activity of oxidative stress markers. [28,29] In this study, HFFD feeding in TP rats resulted in elevated levels of oxidative stress markers and decreased levels of enzymatic and non-enzymatic antioxidants as compared to normal diet fed TP rats which can be attributed to hormonal changes induced by TP and metabolic changes induced by HFFD.…”

Section: Discussionmentioning

confidence: 66%

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Sitagliptin recuperates oxidative stress and inflammatory cytokine expression in ovary of PCOS rats

Carani

Nanda²

2019

J. Drug Delivery Ther.

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Abstract Introduction: Polycystic ovary syndrome (PCOS) is an endocrine, reproductive and metabolic disorder and a major cause of infertility in women. Testosterone propionate (TP) is used to induce PCOS in rats. High calorie diet causes metabolic changes, oxidative stress and PCOS. Sitagliptin (STG) is an inhibitor of dipeptide peptidase (DPP) 4 enzyme used in the treatment of type 2 diabetes. Objective: The aim of the study is to investigate the effect of high fat, high fructose diet (HFFD) on TP induced PCOS rats and the role of STG on oxidative stress and inflammation in PCOS. Materials and methods: PCOS was induced by administration of TP to normal pellet and HFFD fed rats for 43 days. STG (i.p.) was given for the last 15 days to both groups of rats. Vaginal smear, parameters of oxidative stress, antioxidants and inflammation (TNF-α and IL-6) in ovary were analyzed. Results: Vaginal smear from TP rats consisted of persistent leucocytes, a characteristic of PCOS. All the TP administered rats registered significanty elevated levels of glucose, lipids, oxidative stress and inflammatory markers, and reduced levels of antioxidants compared to CON rats. STG treatment to PCOS rats reduced hyperglycemia and hyperlipidemia, oxidative stress and inflammation and improved estrus cycle. Conclusion: High energy diet aggravated TP-induced changes in oxidative stress and inflammatory cytokines in ovary. STG recuperated the changes induced by TP, suggesting that STG holds potential for PCOS management.

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Section: Discussionmentioning

confidence: 66%

“…STG effect, antioxidant and anti-inflammatory potential of STG have also been reported. [13,14] Animal models of PCOS use androgens to induce PCOS. Recently, it is shown that addition of high fat diet to dihydroepiandrostenedione (DHEA) produced exaggerated endocrine and metabolic changes than when DHEA was administered alone.…”

Section: Introductionmentioning

confidence: 99%

Sitagliptin recuperates oxidative stress and inflammatory cytokine expression in ovary of PCOS rats

Carani

Nanda²

2019

J. Drug Delivery Ther.

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“…However, the mechanism of the enhanced oxidative stress in obesity is currently poorly understood, and evidence on links between uremia-related oxidative stress and MM levels is unclear; recently, it swas reported that β2-microglobulin, a traditional MM marker, may not by itself contribute to the pathogenesis of CKD by induction of leukocyte oxidative stress [24]. It was evidenced that oxidative stress is associated with increased expression of genes encoding NADPH oxidase subunits and reduced expression of enzymatic antioxidants genes [25]. Finally, another reason for the increasing of the MM level in obesity condition may be a violation of renal excretory capacity [26][27][28][29][30].…”

Section: Eis Markers In Obesitymentioning

confidence: 99%

Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

et al. 2017

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Background Worldwide obesity spread is a global health problem and needs to be further studied. Co-morbidities of obesity include insulin resistance, diabetes mellitus type 2, and dyslipidemia, which are the most frequent contributing factors for metabolic syndrome (MetS), as well as nonalcoholic fatty liver disease and chronic kidney disease. The aim was to study renal function and endogenous intoxication panel on high-calorie diet-induced obesity rat model and perform comparative study of the treatment efficacy of Fenugreek-based bionanocomposite vs antiobesogenic drugs (Orlistat). Materials We included 60 male rats and equally divided them to 6 groups of 10 animals in each group: the experimental groups were firstly assigned as controls and high caloric diet (HCD)-fed groups, and each group further was subdivided to remain untreated, Fenugreek bionanocomposite (BNC)-treated, and Orlistat-treated. Normal control rats (groups 1, 2, 3) were fed by a standard chow, while the others (groups 4, 5, 6) were fed with HCD ad libitum during 98 days. From days 77 to 98, groups 2 and 5 were treated with BNC based on Fenugreek (150 mg/kg body weight, orally) and groups 3 and 6 were treated with antiobesogenic drug Orlistat (10 mg/kg body weight, orally). Food and water consumptions were measured daily and body weights were measured once a week. On day 99, blood was collected; the creatinine, urea, and uric acid were estimated in serum according to the standard protocols. Levels of low and middle molecules (MMs) were measured; the quantity of oligopeptides was estimated by Bradford method. We performed the liver and kidney ultrasonography in rats.Results We revealed an increase in the levels of endogenous intoxication syndrome markers (MM and oligopeptides) in all animals with experimental obesity. Ultrasound data showed injury of the liver and kidneys in obese rats. We observed significant decreasing of MM levels after Orlistat treatment vs controls (p < 0.05). However, this effect was more pronounced in Fenugreek BNC-treated group vs both Orlistattreated and controls (p < 0.05). Orlistat treatment evoked rising of serum creatinine and oligopeptides in control animals

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“…In animal studies, apigenin has been shown to cross the blood-brain barrier. It has potent anti-oxidant, anti-inflammatory, and anti-apoptotic properties (23,24). In murine microglia that have been activated 6 by interferon gamma, apigenin decreased the levels of IL-6 and TNF-alpha via its effect on phosphorylation of STAT1 (25).…”

Section: Introductionmentioning

confidence: 99%

“…Apigenin and luteolin had significant anxiolytic effects in the elevated plus maze, forced swim test and tail suspension tests(87- 90). In other rodent models of neurodegenerative diseases (rotenone-induced Parkinson disease and24 streptozotocin-induced Alzheimer disease models), epilepsy and cerebral ischemia, apigenin and luteolin significantly improved hippocampal spatial memory in the Morris water maze test(28, 68, 91- 95).Even though treatment with a high dose of apigenin improved exploratory behavior and hippocampal learning, it had negative effects on motor coordination in both Ts1Cje males and females.Anusha et al (68) demonstrated beneficial effects of apigenin treatment at low doses (10 and 20 mg/kg, i.p) on motor coordination in a rotenone-induced rat model of Parkinson disease. However, treatment…”

mentioning

confidence: 97%

Apigenin as a Candidate Prenatal Treatment for Trisomy 21: Effects in Human Amniocytes and the Ts1Cje Mouse Model

Guedj

Pennings

Siegel

et al. 2018

Preprint

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Phone: (+1) 301-496-3454 KEY WORDS: Down syndrome, trisomy 21, prenatal treatment, transcriptome, apigenin, intellectual disability 3 ABSTRACTHuman fetuses with trisomy 21 (T21) have atypical brain development that is apparent sonographically in the second trimester. Prenatal diagnosis provides a potential opportunity to begin treatment in utero.We hypothesize that by analyzing and integrating dysregulated gene expression and pathways common to humans with DS and mouse models we can discover novel targets for therapy. Here, we tested the safety and efficacy of apigenin (4', 5, 7-trihydroxyflavone), identified using this approach, in both human amniocytes from fetuses with T21 and in the Ts1Cje mouse model. The experiments compared treated to untreated results in T21 and euploid cells, as well as in Ts1Cje mice and their wild-type littermate controls. T21 cells cultured with apigenin (2µM) had significantly reduced oxidative stress and improved antioxidant defense response in vitro. Apigenin (333-400 mg/kg/day), mixed with chow, was initiated prenatally to the dams and fed to the pups over their lifetimes. There was no significant increase in birth defects or pup deaths resulting from prenatal apigenin treatment. Apigenin significantly improved several developmental milestones and spatial olfactory memory in Ts1Cje neonates. In addition, we noted sex-specific effects on exploratory behavior and long-term hippocampal memory in adult mice, with males showing significantly more improvement than females. Global gene expression analyses demonstrated that apigenin targets similar signaling pathways through common upstream regulators both in vitro and in vivo. These studies provide proofof-principle that apigenin has therapeutic effects in preclinical models of Down syndrome.

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Apigenin attenuates hippocampal oxidative events, inflammation and pathological alterations in rats fed high fat, fructose diet

Cited by 33 publications

References 26 publications

Sitagliptin recuperates oxidative stress and inflammatory cytokine expression in ovary of PCOS rats

Sitagliptin recuperates oxidative stress and inflammatory cytokine expression in ovary of PCOS rats

Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

Apigenin as a Candidate Prenatal Treatment for Trisomy 21: Effects in Human Amniocytes and the Ts1Cje Mouse Model

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