Apigenin Inhibits Immunostimulatory Function of Dendritic Cells: Implication of Immunotherapeutic Adjuvant

Abstract

Apigenin, one of the most common flavonoids, has been shown to possess anti-inflammatory, anticarcinogenic, and free radical-scavenging properties. However, the influence of apigenin on the immunostimulatory effects and maturation of dendritic cells (DC) remains, for the most part, unknown. In this study, we have attempted to ascertain whether apigenin influences the expression of surface molecules, dextran uptake, cytokine production, and T-cell differentiation as well as the signaling pathways underlying these phenomena in murine bone marrowderived DC. In the presence of apigenin, CD80, CD86, and major histocompatibility complex class I and II molecules, expressions on DC were significantly suppressed, and lipopolysaccharide (LPS)-induced interleukin (IL)-12 expression was impaired. The DC proved highly efficient at antigen capture, as evidenced by the observation of mannose receptor-mediated endocytosis in the presence of apigenin. The LPS-induced activation of mitogen-activated protein kinase, the nuclear translocation of its nuclear factor-B p65 subunit, and the induction of the T-helper 1 response were all impaired in the presence of apigenin, whereas the cell-mediated immune response remained normal. These findings provide new insight into the immunopharmacological functions of apigenin and its effects on DC, and they may also prove useful in the development of adjuvant therapies for individuals suffering from acute or chronic DC-associated diseases.

Health Benefits of Phenolic Compounds Against Cancers

“…Other studies demonstrate the ability of lavonoids as NF-κB inhibitors and their role in preventing NF-κB signaling pathway-mediated disorders. It is identiied that apigen, quercetin, kaempferol, rutin is a potent inhibitor of NF-κB, which may perform a pivotal function in the regulation of cell growth, apoptosis and the regulation of the cell cycle [57][58][59]. Overall, the results indicated that lavonoids suppress the activation of NF-κB and NF-κB-regulated gene expression, leading to enhancement of apoptosis.…”

Sulforaphane inhibits the Th2 immune response in ovalbumin-induced asthma

“…It has been well characterized that in chronic asthma, Th2 lymphocytes infiltrate the lungs and produce inflammatory cytokines, including IL-4, IL-5, and IL-13 (21). These cytokines may induce the expression of inflammatory molecules in both the endothelial cells of the bronchial circulation and the epithelial cells in the airway, and they may also promote the recruitment of lymphocytes and eosinophils (22).…”

Irisflorentin Modifies Properties of Mouse Bone Marrow-Derived Dendritic Cells and Reduces the Allergic Contact Hypersensitivity Responses

“…The cell suspension was then washed twice with PBS for 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE) labeling. CFSE labeling of responder cells was undertaken as previously described (6,37). The cells were resuspended in 1 mM CFSE (Molecular Probes, Eugene, OR, USA) in PBS and periodically shaken at room temperature for 10 min.…”