Apios americana Medik flowers extract protects PC12 cells against H2O2 induced neurotoxicity via regulating autophagy

Chu, Qiang; Yu, Lushuang; Zheng, Zihuan; Meng, Chen; Zheng, Heng; Hang, Miaojia; Li, Yonglu; Li, Xixi; Liu, Yangyang; Yang, Yunyun; Zheng, Xiaodong

doi:10.1016/j.fct.2018.12.003

Cited by 31 publications

(14 citation statements)

References 49 publications

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“…After treatments, LO2 cells were treated with 5 μ M DCF-DA, 5 μ M DHE, 40 μ M NDA, 10 μ M RH123, 50 nM LysoTracker Green, 10 μ g/mL Nile Red, and 0.1 μ M DAPG in serum-free DMEM for 30 min at 37°C to determine intracellular ROS production, O 2 − generation, glutathione (GSH) depletion, mitochondrial membrane potential, lysosome, oil droplet, and autophagosome. Subsequently, the medium was replaced by fresh medium without phenol red and immediately detected by a fluorescence microscope at the same exposure time [27].…”

Section: Methodsmentioning

confidence: 99%

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Chu

Zhang

Chen

et al. 2019

Oxidative Medicine and Cellular Longevity

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Nonalcoholic fatty liver disease (NAFLD) is a common chronic disease that threatens human health, and present therapies remain limited due to the lack of effective drugs. Lipid metabolic disturbance and oxidative stress have strong links to the development of NAFLD, while autophagy was generally accepted as a key regulatory mechanism on these steps. Our previous studies indicated that cherry anthocyanins (CACN) protected against high fat diet-induced obesity and NALFD in C57BL/6 mice, while the underlying molecule mechanism is still unclear. Thus, in this study, we show that CACN protect against oleic acid- (OA-) induced oxidative stress and attenuate lipid droplet accumulation in NAFLD cell models. According to the results of a transmission electron microscope (TEM), western blot, immunofluorescence (IF), and adenovirus transfection (Ad-mCherry-GFP-LC3B), autophagy is in accordance with the lipid-lowering effect induced by CACN. Further studies illustrate that CACN may activate autophagy via mTOR pathways. In addition, an autophagy inhibitor, 3-methyladenine (3-MA), was applied and the result suggested that autophagy indeed participates in the lipid clearance process in OA-induced lipid accumulation. All these results indicate that the positive effects of CACN on OA-induced hepatic lipid accumulation are mediated via activating autophagy, showing a potential target for the therapeutic strategy of NAFLD.

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Section: Methodsmentioning

confidence: 99%

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Chu

Zhang

Chen

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…could also increase the levels of GSH in cells, which is beneficial to HepG2 cells' resistance to H 2 O 2 -induced oxidative stress [22].…”

Section: Ale Ameliorates Lps-inducedmentioning

confidence: 99%

“…There are many signal pathways concerned with the regulation of autophagy as previously reported [33]. HMGB1, FoxO1, and Akt-mTOR are the key regulators to control autophagy [22,[34][35][36]. Researchers have proven that HMGB1 facilitates the phosphorylation of Bcl-2 to promote the dissociation of Beclin1 from the Beclinl-Bcl2 compound and the combination to the autophagy regulator Beclin1, thereby contributing to the assembly of the PI3KC3-Beclin1 compound and autophagy [37].…”

mentioning

confidence: 99%

Flavonoids from Apios americana Medikus Leaves Protect RAW264.7 Cells against Inflammation via Inhibition of MAPKs, Akt-mTOR Pathways, and Nfr2 Activation

Chu

Jia

et al. 2019

Oxidative Medicine and Cellular Longevity

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Apios americana Medikus was once widely accepted as staple food in India for a long time, and the tuber of which possesses high nutrients. During the past decades, most of the research has focused on the biological activity in the tubers of Apios americana Medikus whereas the leaves were ignored. In this study, the Apios americana Medikus leaf extract (ALE) was obtained and seven compounds were identified. LPS-induced RAW264.7 cells were used to study the anti-inflammation activity of ALE. As expected, ALE reduced the secretion of nitric oxide (NO) and inflammatory cytokines via inhibition of NF-κB and MAPK signaling together with activation of Nrf2-Keap1 and FOXO pathways, as well as alleviating the oxidative stress and mitochondrial dysfunction. In addition, ALE could activate HMGB1-Beclin1 and Sirt1-FoxO1 pathways and inhibit the Akt-mTOR signaling pathway to activate autophagy, protecting RAW264.7 cells from inflammation. In summary, our results suggested that ALE might help activate the anti-inflammation system, resulting in the prevention of LPS-induced damage in RAW264.7 cells.

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“…Many studies indicated that oxidative stress is closely associated with most neurodegenerative diseases, cardiovascular diseases and metabolic diseases [2][3][4]. At the cellular level, ROS can induce cell apoptosis via peroxidative damage to DNA, lipids and proteins [5]. The elimination of ROS from cells depends on both exogenous antioxidants, e. g., vitamin C and curcumin, and cellular antioxidative defense system, which includes endogenous antioxidants, such as glutathione (GSH), and cytoprotective proteins.…”

Section: Introductionmentioning

confidence: 99%

Analogs of imine resveratrol alleviate oxidative stress‐induced neurotoxicity in PC12 cells via activation of Nrf2

et al. 2021

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Apios americana Medik flowers extract protects PC12 cells against H2O2 induced neurotoxicity via regulating autophagy

Cited by 31 publications

References 49 publications

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Flavonoids from Apios americana Medikus Leaves Protect RAW264.7 Cells against Inflammation via Inhibition of MAPKs, Akt-mTOR Pathways, and Nfr2 Activation

Analogs of imine resveratrol alleviate oxidative stress‐induced neurotoxicity in PC12 cells via activation of Nrf2

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Apios americana Medik flowers extract protects PC12 cells against H2O2 induced neurotoxicity via regulating autophagy

Cited by 31 publications

References 49 publications

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Cherry Anthocyanins Regulate NAFLD by Promoting Autophagy Pathway

Flavonoids from Apios americana Medikus Leaves Protect RAW264.7 Cells against Inflammation via Inhibition of MAPKs, Akt-mTOR Pathways, and Nfr2 Activation

Analogs of imine resveratrol alleviate oxidative stress‐induced neurotoxicity in PC12 cells via activation of Nrf2

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Apios americana Medik flowers extract protects PC12 cells against H2O2 induced neurotoxicity via regulating autophagy