Aplysia kurodai -derived glycosaminoglycans increase the phagocytic ability of macrophages via the activation of AMP-activated protein kinase and cytoskeletal reorganization in RAW264.7 cells

Ryu, Jinhyun; Sung, Jina; Xie, Chengliang; Shin, Min-Kyoung; Kim, Chang-Woon; Kim, Nam-Gil; Choi, Yeung Joon; Choi, Byeong Dai; Kang, Sang Soo; Kang, Dawon

doi:10.1016/j.jff.2016.08.059

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…The phagocytic ability of macrophages was analyzed using a phagocytosis assay kit (Cayman Chemical) according to a previously described method [18]. Briefly, 5 × 10 4 cells/well RAW 264.7 cells were cultured in a 96-well black-bottomed plate.…”

Section: Phagocytosis Assaymentioning

confidence: 99%

Sea Hare Hydrolysate-Induced Reduction of Human Non-Small Cell Lung Cancer Cell Growth through Regulation of Macrophage Polarization and Non-Apoptotic Regulated Cell Death Pathways

Nyiramana

Cho

Kim

et al. 2020

Cancers

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Sea hare-derived compounds induce macrophage activation and reduce asthmatic parameters in mouse models of allergic asthma. These findings led us to study the role of sea hare hydrolysates (SHH) in cancer pathophysiology. SHH treatment-induced M1 macrophage activation in RAW264.7 cells, peritoneal macrophages, and THP-1 cells, as did lipopolysaccharide (LPS) (+ INF-γ), whereas SHH reduced interleukin (IL)-4 (+IL-13)-induced M2 macrophage polarization. In addition, SHH treatment inhibited the actions of M1 and M2 macrophages, which have anticancer and pro-cancer effects, respectively, in non-small cell lung cancer cells (A549 and HCC-366) and tumor-associated macrophages (TAMs). Furthermore, SHH induced G2/M phase arrest and cell death in A549 cells. SHH also downregulated STAT3 activation in macrophages and A549 cells, and the down-regulation was recovered by colivelin, a STAT3 activator. SHH-induced reduction of M2 polarization and tumor growth was blocked by colivelin treatment. SHH-induced cell death did not occur in the manner of apoptotic signaling pathways, while the death pattern was mediated through pyroptosis/necroptosis, which causes membrane rupture, formation of vacuoles and bleb, activation of caspase-1, and secretion of IL-1β in SHH-treated A549 cells. However, a combination of SHH and colivelin blocked caspase-1 activation. Z-YVAD-FMK and necrostatin-1, pyrotosis and necroptosis inhibitors, attenuated SHH's effect on the cell viability of A549 cells. Taken together, SHH showed Cancers 2020, 12, 726 2 of 24 anticancer effects through a cytotoxic effect on A549 cells and a regulatory effect on macrophages in A549 cells. In addition, the SHH-induced anticancer effects were mediated by non-apoptotic regulated cell death pathways under STAT3 inhibition. These results suggest that SHH may be offered as a potential remedy for cancer immunotherapy.

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Section: Phagocytosis Assaymentioning

confidence: 99%

Sea Hare Hydrolysate-Induced Reduction of Human Non-Small Cell Lung Cancer Cell Growth through Regulation of Macrophage Polarization and Non-Apoptotic Regulated Cell Death Pathways

Nyiramana

Cho

Kim

et al. 2020

Cancers

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“…Even though the role of glycosaminoglycan (GAG), as a main component in SHH, has been unclear in the inflammatory process, several studies have demonstrated its critical role in inflammatory diseases [ 40 , 41 , 42 ]. In our previous study, however, GAG did not show anti-inflammatory effects on lipopolysaccharide (LPS)-activated RAW264.7 macrophage cells [ 7 ]. Carotenoids, lycopene, geraniol, and alkaloids have been studied for their anti-asthmatic effects on reducing oxidative damage [ 43 ], the infiltration of inflammatory immunocytes into the bronchoalveolar lavage [ 44 ], modulation of Th1/Th2 balance and activation of the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element pathway [ 45 ], and bronchodilation, respectively [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

“…Sea hare has a large number of bioactive molecules, and secretes the molecules as a defense mechanism to compensate for the absence of a shell as physical protection [ 3 ]. The bioactive molecules have shown a variety of functions, such as antitumor, antioxidant, antimicrobial, antibacterial, and immunostimulatory activities [ 4 , 5 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Reduction of Asthmatic Parameters by Sea Hare Hydrolysates in a Mouse Model of Allergic Asthma

et al. 2017

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Sea hare has a variety of biological activities. However, little is known regarding the anti-asthmatic effects of sea hare. This study was performed to identify the effect of sea hare hydrolysates (SHH) on an ovalbumin (OVA)-induced allergic asthma model. The experimental asthma model was sensitized and challenged with OVA. We found that a high-dose of SHH (HSHH) significantly inhibited OVA-induced airway inflammation and mucus production around the airway in lung sections, while low- and medium-dose SHH showed an insignificant effect. In addition, HSHH highly reduced OVA-induced production of interleukin-4, -5, -13, leukotriene D4, E4, and histamine in bronchoalveolar lavage fluid. HSHH decreased the histamine-induced increase in the intracellular Ca2+ level and contractions in asthmatic smooth muscle cells. Furthermore, HSHH did not affect the weights of the spleen nor thymus, whereas dexamethasone (DEX), a steroidal anti-inflammatory drug, reduced them. Taken together, these results showed that HSHH reduced asthmatic parameters in a mouse model of allergic asthma, and suggest that SHH could be used as a potential therapeutic agent for asthma.

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“…KSV a (×10 LPS, a major component of the outer Gram-negative bacteria membrane, was used to create the cellular inflammatory condition, and LPS-treated RAW264.7 cells secrete a variety of inflammatory mediators [25]. LPS-treated RAW264.7 cells undergo morphological changes, such as cell enlargement, flattening, cellular spreading, and vacuole formation, which indicates that RAW264.7 cells have been activated [26].…”

Section: Compoundsmentioning

confidence: 99%

“…Molecules 2021, 26, 6602 6 of 13 changes, such as cell enlargement, flattening, cellular spreading, and vacuole formation, which indicates that RAW264.7 cells have been activated [26]. Among the four triterpenoids, 1 and 2 ameliorated the LPS-induced morphological changes in RAW264.7 cells.…”

Section: Compoundsmentioning

confidence: 99%

Iridal-Type Triterpenoids Displaying Human Neutrophil Elastase Inhibition and Anti-Inflammatory Effects from Belamcanda chinensis

et al. 2021

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The aim of this study is to explore anti-inflammatory phytochemicals from B. chinensis based on the inhibition of pro-inflammatory enzyme, human neutrophil elastase (HNE) and anti-inflammatory activities in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage. Three stereoisomers of iridal-type triterpenoids (1–3) were isolated from the roots of B. chinensis and their stereochemistries were completely identified by NOESY spectra. These compounds were confirmed as reversible noncompetitive inhibitors against HNE with IC50 values of 6.8–27.0 µM. The binding affinity experiment proved that iridal-type triterpenoids had only a single binding site to the HNE enzyme. Among them, isoiridogermanal (1) and iridobelamal A (2) displayed significant anti-inflammatory effects by suppressing the expressions of pro-inflammatory cytokines, such as iNOS, IL-1β, and TNF-α through the NF-κB pathway in LPS-stimulated RAW264.7 cells. This is the first report that iridal-type triterpenoids are considered responsible phytochemicals for anti-inflammatory effects of B. chinensis.

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Aplysia kurodai -derived glycosaminoglycans increase the phagocytic ability of macrophages via the activation of AMP-activated protein kinase and cytoskeletal reorganization in RAW264.7 cells

Cited by 8 publications

References 44 publications

Sea Hare Hydrolysate-Induced Reduction of Human Non-Small Cell Lung Cancer Cell Growth through Regulation of Macrophage Polarization and Non-Apoptotic Regulated Cell Death Pathways

Sea Hare Hydrolysate-Induced Reduction of Human Non-Small Cell Lung Cancer Cell Growth through Regulation of Macrophage Polarization and Non-Apoptotic Regulated Cell Death Pathways

Reduction of Asthmatic Parameters by Sea Hare Hydrolysates in a Mouse Model of Allergic Asthma

Iridal-Type Triterpenoids Displaying Human Neutrophil Elastase Inhibition and Anti-Inflammatory Effects from Belamcanda chinensis

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