Apoptosis in the Porcine Uterine Endometrium During the Estrous Cycle, Early Pregnancy and Post Partum

Okano, Akira; Ogawa, Hidehiko; Takahashi, Hitomi; Geshi, Masaya

doi:10.1262/jrd.18139

Cited by 38 publications

(20 citation statements)

References 26 publications

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“…In pigs, a non‐menstruating species, there is repeated endometrial hyperplasia but, in the absence of implantation, apoptosis is observed. Interestingly, the peak of apoptotic cell death in stromal cells occurs on Days 4–8 (Okano et al, ) and in the glandular and luminal epithelium on Day 15 and Days 17–19, respectively, of the pig estrous cycle (Wasowska et al, ). Xu et al () further described increased side‐population cell proliferation after stimulation with 17β‐estradiol, which was inhibited by the estrogen receptor antagonist ICI182780, indicating to these authors that estrogen can directly stimulate side‐population cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

Evidence for the presence of stem/progenitor cells in porcine endometrium

Bodek

Bukowska

Wı́sniewska

et al. 2015

Molecular Reproduction Devel

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A population of adult stem cells responsible for cyclic reconstructing and remodeling has been proposed to reside in the highly regenerative mammalian endometrium. Recently, stem/progenitor cells have been identified in the human and mouse endometrium, but less is known about these cells in livestock animals. Using Hoechst 33342 fluorescent dye staining and flow cytometry, we identified an emerging cell side population that may be responsible for the regeneration process of the porcine endometrium. The percentage of side-population cells on Day 19 of the estrous cycle was significantly higher than that on Days 2-4. Moreover, single cells were able to seed clones that could differentiate into three independent mesenchymal-cell lineages. We also demonstrated the expression of specific markers of self-renewal cells on these side-population cells and the presence of a population of cells among the stromal cells that possess markers for mesenchymal stem cells. These results indicate that the porcine endometrium contains a population of cells with the capacity for self-renewal and a high rate of proliferation, which depend on the phase of the estrous cycle. These cells could potentially be involved in the cyclic reconstruction of the porcine endometrium.

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Section: Discussionmentioning

confidence: 99%

Evidence for the presence of stem/progenitor cells in porcine endometrium

Bodek

Bukowska

Wı́sniewska

et al. 2015

Molecular Reproduction Devel

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“…One of the basic events of uterine cellular change is physiological cell turnover, which involves cell death by apoptosis and cell renewal by proliferation (Spencer et al 1996;Toki et al 1998). Previous studies in several mammalian species have demonstrated an inverse correlation between cell apoptosis and cell proliferation in the uterus (Kokawa et al 1996;Sato et al 1997;Tao et al 1998;Pampfer and Donnay 1999;Dharma et al 2000;Vaskivuo et al 2000;Chu et al 2002Chu et al , 2006Joswig et al 2003;Van Cruchten et al 2003;Harada et al 2004;Wei et al 2005;Narkar et al 2006;Zhang and Paria 2006;Okano et al 2007; Roberto da Costa et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression of Survivin, Bcl‐2 and Bax Proteins in the Domestic Cat (Felis catus) Endometrium During the Oestrus Cycle

Lïman¹,

Alan²,

Bayram³

et al. 2012

Reprod Domestic Animals

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Contents Apoptosis has been shown to be an important regulator of endometrium function. To clarify the regulation of apoptosis in the cat endometrium during the normal oestrus cycle, the expressions of the apoptosis‐related proteins (Bcl‐2 and Bax) and their correlation to the inhibitor of apoptosis protein Survivin were analysed using immunohistochemistry. The TUNEL technique (TdT‐mediated dUTP nick end labelling) was also used to detect DNA fragmentation characteristic of apoptotic cells. The results demonstrated that TUNEL labelling is not effective for the detection of apoptosis in cat endometrium. Survivin was expressed in the luminal and glandular epithelial cells of cat endometrium during all phases of the oestrus cycle. Survivin was localized in both the cytoplasm and nuclei of superficial and deep uterine gland cells during the luteal phase, while only cytoplasmic staining was observed during the follicular and anoestrus phases. Bax immunoreactivity in the cytoplasm of luminal and glandular epithelial cells as well as the smooth muscle cells of blood vessels was weak in the anoestrus phase. Compared with anoestrus, the intensity of Bax immunostaining was moderate in the follicular phase and increased dramatically in the luteal phase. Bcl‐2 immunostaining in the cytoplasm of luminal and glandular epithelial cells was moderate in the anoestrus phase. During the early follicular phase, cytoplasmic Bcl‐2 immunostaining was detected mostly in glandular epithelial cells. In the mid‐follicular phase, in glands, the amount of Bcl‐2 protein increased progressively from the superficial to the deep layer. In contrast, the expression of Bcl‐2 decreased in the secretory phase, being very low or absent in the mid‐ and late luteal phases. The overall results suggest that Survivin, Bax and Bcl‐2 proteins may cooperatively contribute to cell apoptosis and cell proliferation in the cat uterus during the oestrus cycle.

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“…It has been demonstrated that apoptosis plays an important role in human trophoblastic villi differentiation and syncytium formation (Huppertz, Kadyrov, & Kingdom, ). In sows, at the beginning of gestation, apoptosis has been related to placental villi homoeostasis through cell remodelling (Cristofolini et al, ; Merkis, Cristofolini, Sanchis, & Koncurat, ; Okano, Ogawa, Takahashi, & Geshi, ) while at the end of human and swine pregnancy, cell remodelling by apoptosis would be contributing to foetal and maternal membranes separation (Barrio et al, ; Cristofolini et al, ). The first findings of placental cell remodelling by apoptosis in at term caprine placenta were described by our research group (Cristofolini, Turiello, Sanchis, Cufre, & Merkis, ).…”

Section: Introductionmentioning

confidence: 99%

Effect of prepubertal nutrition on cellular apoptosis and proliferation in at term placenta of Anglo‐Nubian goats

Turiello

Cristofolini

Fiorimanti

et al. 2019

Reprod Domestic Animals

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Contents The aim of this study was to evaluate the effect of feed restriction with or without monensin supplementation, followed by a re‐feeding period on cellular apoptosis and proliferation in at term placenta of Anglo‐Nubian goats. To evaluate the induction of apoptosis through the intrinsic pathway, proteins Bax and Bcl‐2 were determinated. The apoptosis was related with the cell proliferation indices through Ki67 determination. The treatments were applied for 250 days and were (a) ad libitum feeding (control; n = 5); (b) restricted feeding at 70% of control (restricted; n = 7); and (c) restricted with monensin supplementation (monensin; n = 7). After treatments, all the animals were fed to support their requirements. After parturition, 27 placentas were gathered. The placental cellular structure was studied by high‐resolution light microscopy and transmission electron microscopy; the cellular proliferation was determined by Ki67 index, and Bax and Bcl‐2 proteins were localized by immunohistochemical analysis. Differences in cell proliferation through the Ki67 index were found in monensin group placentas. Monensin supplementation stimulated the placental cell proliferation reversing the effect of feed restriction during the peripuberal period. A significant increase of Bcl‐2 in placentas of restricted group was found, and it would provide a protective effect on the placental structure. A lack of the Bcl‐2 protective effect was observed in control and monensin group placentas, probably meaning that the observed apoptosis would be induced through the intrinsic signalling pathway. A balance between apoptosis and cell proliferation is necessary to maintain tissue homoeostasis during caprine placental development.

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Apoptosis in the Porcine Uterine Endometrium During the Estrous Cycle, Early Pregnancy and Post Partum

Cited by 38 publications

References 26 publications

Evidence for the presence of stem/progenitor cells in porcine endometrium

Evidence for the presence of stem/progenitor cells in porcine endometrium

Expression of Survivin, Bcl‐2 and Bax Proteins in the Domestic Cat (Felis catus) Endometrium During the Oestrus Cycle

Effect of prepubertal nutrition on cellular apoptosis and proliferation in at term placenta of Anglo‐Nubian goats

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