Apoptosis induced by ZnPcH1-based photodynamic therapy in Jurkat cells and HEL cells

Huang, Huifang; Zhao, Xiaoyan; Chen, Yuanzhong; Long, Rong; Wu, Yong

doi:10.1007/s12185-011-0964-y

Cited by 4 publications

(3 citation statements)

References 34 publications

(32 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we confirmed that PDT is a highly efficient treatment option and rapidly induced cell death owing to excessive free radicals in cancer cells. PDT destroys tumor cells by a process that can produce apoptosis, necrosis, and an acute inflammation (26, 27). Although PDT is known to be a promising new therapy, tumor recurrences and PDT resistance can occur.…”

Section: Discussionmentioning

confidence: 99%

Down‐regulation of heat‐shock protein 27–induced resistance to photodynamic therapy in oral cancer cells

Kim

Jung

Lim

et al. 2012

J Oral Pathology Medicine

View full text Add to dashboard Cite

Photodynamic therapy (PDT) of cells is a new treatment modality involving selective delivery of a photosensitive dye into target cells, followed by visible light irradiation. PDT induces cell death by excessive ROS generation. The effects of multiple photosensitizers were owing to the difference in cell types involving sensitizer-specific protein changes linked to resistance. HSP27 is regulated in response to stress and is associated with apoptotic process. The effects of HSP27 on PDT resistance are controversial and unclear. The purpose of this study was to investigate the role of HSP27 down-regulation in the PDT-induced cells and HSP27 regulation in the resistance to PDT. KB cells transfected with HSP27 siRNA were exposed to hematoporphyrin (HP) followed by irradiation at 635 nm at an energy density of 4.5 mW/cm(2). After irradiation, the effects on HSP27 down-regulation were assessed by MTT assay, flow cytometry, confocal analysis, Western blotting and caspase activity. The results of this study showed that down-regulation of HSP27 restored cell survival in HP-PDT-induced apoptotic KB cells. HSP27 down-regulation attenuated PDT-induced apoptosis through caspase-mediated pathway in KB cells. Also, HSP27 silencing regulated Bax, Bcl-2, and PARP protein expression in PDT-induced cells. Therefore, HSP27 down-regulation confers resistance to PDT through interruption of apoptotic protein activity in PDT-induced cell death. HSP27 might contribute to regulating PDT-induced apoptosis in PDT-resistant cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Down‐regulation of heat‐shock protein 27–induced resistance to photodynamic therapy in oral cancer cells

Kim

Jung

Lim

et al. 2012

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…Several studies have demonstrated that Caspase-3, Bcl-2 family proteins, and mitochondria are involved in phthalocyanines-PDT-induced apoptosis of cancer cells [18][19][20][21][22]. Our previous study has showed that Bcl-2 is involved in T PcZn-PDT-induced apoptosis of Bel-7402 cells [11].…”

Section: Discussionmentioning

confidence: 96%

“…molecules. Recent studies have demonstrated that induction of proapoptotic proteins and inhibition of antiapoptotic proteins can be caused by phthalocyanines-PDT in apoptosis of cancer cells [11,21,22].…”

Section: Introductionmentioning

confidence: 99%

Autoregulatory Feedback Mechanism of P38MAPK/Caspase-8 in Photodynamic Therapy-Hydrophilic/Lipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells

Wang

Xia

Chen

et al. 2014

International Journal of Photoenergy

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is a novel and promising antitumor treatment. Our previous study showed that hydrophilic/lipophilic tetra--(4-carboxyphenoxy) phthalocyanine zinc-(T PcZn-) mediated PDT (T PcZn-PDT) inhibits the proliferation of human hepatocellular carcinoma Bel-7402 cells by triggering apoptosis and arresting cell cycle. However, mechanisms of T PcZn-PDTinduced apoptosis of Bel-7402 cells have not been fully clarified. In the present study, therefore, effect of T PcZn-PDT on apoptosis, P38MAPK, p-P38MAPK, Caspase-8, Caspase-3, Bcl-2, Bid, Cytochrome c, and mitochondria membrane potential in Bel-7402 cells without or with P38MAPK inhibitor SB203580 or Caspase-8 inhibitor Ac-IEFD-CHO was investigated by haematoxylin and eosin (HE) staining assay, flow cytometry analysis of annexin V-FITC/propidium iodide (PI) double staining cells and 5,5 ,6,6tetrachloro-1,1 ,3,3 -tetraethylbenzimidazolylcarbocyanine iodide (JC-1), and immunoblot assay. We found that T PcZn-PDT resulted in apoptosis induction, activation of P38MAPK, Caspase-8, Caspase-3, and Bid, downregulation of Bcl-2, release of Cytochrome c from mitochondria, and disruption of mitochondrial membrane potential in T PcZn-PDT-treated Bel-7402 cells. In contrast, SB203580 or Ac-IEFD-CHO attenuated induction of apoptosis, activation of P38MAPK, Caspase-8, Caspase-3, and Bid, downregulation of Bcl-2, release of Cytochrome c from mitochondria, and disruption of mitochondrial membrane potential in T PcZn-PDT-treated Bel-7402 cells. Taken together, we conclude that Caspase-3, Bcl-2, Bid, and mitochondria are involved in autoregulatory feedback of P38MAPK/Caspase-8 during T PcZn-PDT-induced apoptosis of Bel-7402 cells.

show abstract

Intracellular distribution and mechanisms of actions of photosensitizer Zinc(II)-phthalocyanine solubilized in Cremophor EL against human hepatocellular carcinoma HepG2 cells

et al. 2013

View full text Add to dashboard Cite

Apoptosis induced by ZnPcH1-based photodynamic therapy in Jurkat cells and HEL cells

Cited by 4 publications

References 34 publications

Down‐regulation of heat‐shock protein 27–induced resistance to photodynamic therapy in oral cancer cells

Down‐regulation of heat‐shock protein 27–induced resistance to photodynamic therapy in oral cancer cells

Autoregulatory Feedback Mechanism of P38MAPK/Caspase-8 in Photodynamic Therapy-Hydrophilic/Lipophilic Tetra-α-(4-carboxyphenoxy) Phthalocyanine Zinc-Induced Apoptosis of Human Hepatocellular Carcinoma Bel-7402 Cells

Intracellular distribution and mechanisms of actions of photosensitizer Zinc(II)-phthalocyanine solubilized in Cremophor EL against human hepatocellular carcinoma HepG2 cells

Contact Info

Product

Resources

About